Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilN   Type   Machinery gene
Locus tag   BCV29_RS08855 Genome accession   NZ_CP170001
Coordinates   1977426..1978040 (-) Length   204 a.a.
NCBI ID   WP_010435288.1    Uniprot ID   -
Organism   Vibrio cyclitrophicus strain 10N.286.55.B4     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1972426..1983040
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BCV29_RS08830 (BCV29_08830) aroB 1972740..1973828 (-) 1089 WP_010435273.1 3-dehydroquinate synthase -
  BCV29_RS08835 (BCV29_08835) aroK 1973853..1974371 (-) 519 WP_009848907.1 shikimate kinase AroK -
  BCV29_RS08840 (BCV29_08840) pilQ 1974548..1976239 (-) 1692 WP_102312935.1 type IV pilus secretin PilQ Machinery gene
  BCV29_RS08845 (BCV29_08845) pilP 1976299..1976817 (-) 519 WP_016767989.1 pilus assembly protein PilP Machinery gene
  BCV29_RS08850 (BCV29_08850) pilO 1976804..1977433 (-) 630 WP_010435284.1 type 4a pilus biogenesis protein PilO Machinery gene
  BCV29_RS08855 (BCV29_08855) pilN 1977426..1978040 (-) 615 WP_010435288.1 PilN domain-containing protein Machinery gene
  BCV29_RS08860 (BCV29_08860) pilM 1978024..1979049 (-) 1026 WP_016767986.1 type IV pilus assembly protein PilM Machinery gene
  BCV29_RS08865 (BCV29_08865) - 1979201..1981759 (+) 2559 WP_198589774.1 penicillin-binding protein 1A -
  BCV29_RS08870 (BCV29_08870) oxyR 1981855..1982748 (-) 894 WP_010435297.1 DNA-binding transcriptional regulator OxyR -

Sequence

Protein


Download         Length: 204 a.a.        Molecular weight: 23345.82 Da        Isoelectric Point: 9.3072

>NTDB_id=1054290 BCV29_RS08855 WP_010435288.1 1977426..1978040(-) (pilN) [Vibrio cyclitrophicus strain 10N.286.55.B4]
MLHQINLLSWRDEIRAQHKKRFIHLVILGVIIALAGQWAVGHYFHDQQAKQQARLSYLDKYIAVLDRQIQSLKVAEQEHK
AILTRLAVVESLQFGRNKTTDFMNLMPELIPEGVYVDKIKMNGQEIEMSGISDSTARLATMLDNLERSDSLKEVEMHSIV
HNRKRFNKEFQSFKVSFVFSPVSLENTTAEGTTANIKEKGANHG

Nucleotide


Download         Length: 615 bp        

>NTDB_id=1054290 BCV29_RS08855 WP_010435288.1 1977426..1978040(-) (pilN) [Vibrio cyclitrophicus strain 10N.286.55.B4]
ATGTTGCATCAAATTAACCTGTTGTCGTGGCGTGATGAGATTCGCGCTCAGCATAAAAAGCGTTTCATTCATCTGGTCAT
TCTTGGCGTCATCATTGCGCTTGCCGGGCAATGGGCGGTGGGACACTATTTTCATGACCAACAAGCCAAGCAGCAAGCGC
GCCTCAGTTATCTCGATAAGTACATCGCAGTGCTCGACCGACAAATCCAGTCATTAAAAGTCGCAGAGCAAGAGCACAAA
GCCATCCTTACACGACTTGCTGTCGTGGAGTCTTTACAGTTCGGGCGCAATAAAACCACCGACTTTATGAACCTGATGCC
GGAGCTGATTCCTGAGGGCGTGTATGTCGACAAGATAAAGATGAATGGTCAGGAGATTGAAATGTCGGGCATCAGCGACA
GCACCGCTCGTCTAGCCACCATGTTGGACAACCTAGAACGTTCTGACTCTCTTAAAGAAGTTGAGATGCACTCTATTGTT
CATAACCGTAAGCGCTTCAATAAAGAGTTTCAGAGCTTCAAGGTCTCTTTTGTGTTTAGCCCTGTTTCTTTAGAAAACAC
AACCGCAGAGGGCACAACTGCGAACATAAAAGAGAAGGGGGCGAATCATGGCTAA

Domains

Predicted by InterproScan.

Fimbrial assembly protein (PilN) (PilN)

(103-176)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilN Vibrio cholerae strain A1552

63.731

94.608

0.603
  pilN Vibrio campbellii strain DS40M4

61.78

93.627

0.578

Multiple sequence alignment