Detailed information NTG1054291

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: BCV29_RS08860
• Genome accession: NZ_CP170001
• Coordinates: 1978024..1979049 (-)
• Length: 341 a.a.
• NCBI ID: WP_016767986.1
• Uniprot ID: -
• Organism: Vibrio cyclitrophicus strain 10N.286.55.B4
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1973024..1984049

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
BCV29_RS08835 (BCV29_08835)	aroK	1973853..1974371 (-)	519	WP_009848907.1	shikimate kinase AroK	-
BCV29_RS08840 (BCV29_08840)	pilQ	1974548..1976239 (-)	1692	WP_102312935.1	type IV pilus secretin PilQ	Machinery gene
BCV29_RS08845 (BCV29_08845)	pilP	1976299..1976817 (-)	519	WP_016767989.1	pilus assembly protein PilP	Machinery gene
BCV29_RS08850 (BCV29_08850)	pilO	1976804..1977433 (-)	630	WP_010435284.1	type 4a pilus biogenesis protein PilO	Machinery gene
BCV29_RS08855 (BCV29_08855)	pilN	1977426..1978040 (-)	615	WP_010435288.1	PilN domain-containing protein	Machinery gene
BCV29_RS08860 (BCV29_08860)	pilM	1978024..1979049 (-)	1026	WP_016767986.1	type IV pilus assembly protein PilM	Machinery gene
BCV29_RS08865 (BCV29_08865)	-	1979201..1981759 (+)	2559	WP_198589774.1	penicillin-binding protein 1A	-
BCV29_RS08870 (BCV29_08870)	oxyR	1981855..1982748 (-)	894	WP_010435297.1	DNA-binding transcriptional regulator OxyR	-
BCV29_RS08875 (BCV29_08875)	-	1982970..1983698 (+)	729	WP_010435300.1	glutathione peroxidase	-

Sequence

Protein

• Length: 341 a.a.
• Molecular weight: 38112.77 Da
• Isoelectric Point: 6.8348

>NTDB_id=1054291 BCV29_RS08860 WP_016767986.1 1978024..1979049(-) (pilM) [Vibrio cyclitrophicus strain 10N.286.55.B4]
MGSSLITGIDINHHSIKAVVLKPVGESYALVGYKELPISDDIFTANHTLEYQKTVKKLKELRKGLPFGSRNVAISVPDNT
VISKVLQIESELEDREKEFSIYQTFAHQSPFPIEELSLDFVKLEDKRFGKGATSSYQVYATRKEVVDSRADALTKAGFKP
IVVDTQAHGLLNIWQLASHLYPEKKNWLLVDVGVDQTSLGIIPQGSAPFYKDIAFGIQDLCSTGNSMDMEGGFSNVEEKH
KFIVNLIEKLKRQLQLYSSVNALQPISGIWLMGEGASMPMVVEELERHFQLSCESLNPLSLFENKVAKKRRLPMDWQHFG
IAAGMAMSGLKWQGEKHVASN

Nucleotide

• Length: 1026 bp

>NTDB_id=1054291 BCV29_RS08860 WP_016767986.1 1978024..1979049(-) (pilM) [Vibrio cyclitrophicus strain 10N.286.55.B4]
ATGGGTTCATCATTAATTACAGGTATAGATATAAATCACCACAGCATCAAAGCCGTGGTACTAAAACCCGTTGGGGAGTC
GTATGCCCTGGTGGGATACAAAGAGCTGCCGATTTCGGACGACATTTTTACAGCTAACCATACTTTGGAGTATCAGAAAA
CTGTTAAGAAACTCAAAGAACTTAGGAAAGGGCTGCCTTTTGGAAGCCGCAACGTCGCTATTTCGGTACCGGATAACACG
GTGATCAGCAAAGTACTGCAAATAGAGAGCGAATTAGAAGATAGAGAAAAGGAGTTCTCGATCTATCAAACCTTTGCTCA
TCAATCTCCCTTTCCTATCGAGGAGCTGAGTTTAGATTTTGTAAAGCTAGAAGACAAACGATTTGGTAAAGGAGCAACAA
GCAGCTATCAAGTGTATGCGACTCGTAAAGAGGTGGTTGATAGCCGAGCTGACGCGCTAACGAAAGCAGGGTTTAAGCCC
ATCGTTGTCGACACTCAGGCCCATGGTTTACTCAACATTTGGCAATTGGCTTCGCATTTGTATCCAGAAAAAAAGAATTG
GCTGTTGGTGGATGTTGGCGTTGATCAAACTTCATTAGGCATTATTCCCCAAGGTTCAGCGCCATTTTATAAAGATATTG
CCTTTGGCATTCAAGATCTTTGCAGTACGGGTAACTCGATGGATATGGAGGGTGGGTTTAGTAACGTTGAGGAGAAGCAT
AAGTTTATTGTCAATTTGATTGAAAAACTCAAGCGTCAGTTACAGCTCTATTCATCGGTCAATGCGCTTCAACCTATCTC
AGGAATTTGGCTAATGGGCGAAGGAGCAAGTATGCCGATGGTCGTTGAAGAGTTAGAGCGACATTTTCAGTTAAGTTGTG
AGTCTTTGAACCCCCTATCTTTGTTTGAGAATAAGGTCGCGAAAAAACGTCGATTACCCATGGATTGGCAACATTTTGGT
ATTGCAGCAGGCATGGCGATGAGTGGACTCAAGTGGCAGGGAGAAAAGCATGTTGCATCAAATTAA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (8-220)

• Type IV pilus assembly protein PilM; (PilM_2) (242-328)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Vibrio cholerae strain A1552	53.097	99.413	0.528
pilM	Vibrio campbellii strain DS40M4	47.661	100	0.478