Detailed information NTG1053253

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: ABUS32_RS14710
• Genome accession: NZ_CP169805
• Coordinates: 3048378..3049496 (+)
• Length: 372 a.a.
• NCBI ID: WP_000347035.1
• Uniprot ID: -
• Organism: Acinetobacter baumannii strain HvAb04
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3043378..3054496

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ABUS32_RS14700	-	3046494..3047186 (-)	693	WP_001108510.1	YggS family pyridoxal phosphate-dependent enzyme	-
ABUS32_RS14705	pilT	3047313..3048350 (+)	1038	WP_000355489.1	type IV pilus twitching motility protein PilT	Machinery gene
ABUS32_RS14710	pilU	3048378..3049496 (+)	1119	WP_000347035.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
ABUS32_RS14715	fur	3049566..3050003 (-)	438	WP_001122847.1	ferric iron uptake transcriptional regulator	-
ABUS32_RS14720	-	3050115..3050513 (+)	399	WP_001170994.1	outer membrane protein assembly factor BamE	-
ABUS32_RS14725	-	3050568..3050891 (-)	324	WP_000442585.1	RnfH family protein	-
ABUS32_RS14730	-	3050891..3051958 (-)	1068	WP_005120426.1	hypothetical protein	-
ABUS32_RS14735	-	3052111..3052566 (-)	456	WP_034129532.1	bacteriohemerythrin	-
ABUS32_RS14740	-	3052819..3053667 (-)	849	WP_000336551.1	class II glutamine amidotransferase	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41821.98 Da
• Isoelectric Point: 6.5675

>NTDB_id=1053253 ABUS32_RS14710 WP_000347035.1 3048378..3049496(+) (pilU) [Acinetobacter baumannii strain HvAb04]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRKEFADTRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLADEGPDQLSDGKQHLTFDSQ

Nucleotide

• Length: 1119 bp

>NTDB_id=1053253 ABUS32_RS14710 WP_000347035.1 3048378..3049496(+) (pilU) [Acinetobacter baumannii strain HvAb04]
ATGGATTTTAATGACTTGCTCAACCTGATGGTTGAAAAGAAATCTTCCGATCTCTTTATTACAGATGGCGTTGCGCCCTC
TATGAAGATTAATGGGCAAATTGTTCCAATTTCAAAAAACAGTCTTTCTGGGGAAGTGATTGGTCAACTATTACATTCCA
TCATGAGCGAAAAACAACGCAAAGAATTTGCAGACACTCGTGAATGTAACTTTGCCATTATGAACCGTGAAAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAGCGCGACATGCCGGGCATGGTACTACGTCGTATTGAAACCAAGATTCCTTCAAT
TGATGACTTGCAGTTACCGCCTGTACTTAAAGATTTATCGATGACCAAACGCGGCATTATTATTTTTGTAGGCGCGACAG
GCACAGGTAAATCTACTTCATTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAGGGCCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCACGAACATGCAGGTTGCATCATTACTCAGCGTGAAGTCGGGATCGATACCGACTCATTTGA
AATTGCCTTGAAAAATACACTACGACAAGCGCCGGATGTGATCTTAATTGGTGAGATCCGTTCTCGTGAAGTCATGGACT
ACGCGATTGGCTTTGCTGAAACAGGCCATCTTGTATTAGCTACCATGCACGCTAACAACGCCAACCAAGCGCTCGACCGT
ATTATTCACTTTTTTGAAAGTGACCGTCATAGCCAGCTTTATATGGACTTGTCACTTAACTTAAAGGCTATGATTGCGCA
GCAGCTCATTCCAACACCAGATGGTAATTCACGCCGTGCAGCAATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAAGATTTGATGAAACGCTCACGTGAACTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATATAAAGCTGGTCAAATTACCTACAAAGATGCACTTAAACATGCTGACTCACCGAACGATTTACG
TTTAACCATTAAACTTGCAGATGAAGGTCCTGATCAATTATCAGACGGAAAACAGCATTTAACTTTTGACAGCCAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (34-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.661	100	0.769
pilU	Pseudomonas stutzeri DSM 10701	68.817	100	0.688
pilU	Vibrio cholerae strain A1552	53.243	99.462	0.53
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	41.908	93.011	0.39