Detailed information NTG1052045

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: ACE17X_RS01775
• Genome accession: NZ_CP169561
• Coordinates: 404975..406039 (+)
• Length: 354 a.a.
• NCBI ID: WP_010946662.1
• Uniprot ID: Q5ZX06
• Organism: Legionella pneumophila strain lpg1386
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 399975..411039

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACE17X_RS01765 (ACE17X_01765)	-	401012..403396 (-)	2385	WP_010946660.1	penicillin-binding protein 1A	-
ACE17X_RS01770 (ACE17X_01770)	ravI	403548..404558 (-)	1011	WP_010946661.1	Dot/Icm T4SS effector RavI	-
ACE17X_RS01775 (ACE17X_01775)	pilM	404975..406039 (+)	1065	WP_010946662.1	type IV pilus assembly protein PilM	Machinery gene
ACE17X_RS01780 (ACE17X_01780)	pilN	406043..406591 (+)	549	WP_010946663.1	PilN domain-containing protein	Machinery gene
ACE17X_RS01785 (ACE17X_01785)	pilO	406605..407204 (+)	600	WP_014841277.1	type 4a pilus biogenesis protein PilO	Machinery gene
ACE17X_RS01790 (ACE17X_01790)	pilP	407201..407788 (+)	588	WP_010946665.1	pilus assembly protein PilP	Machinery gene
ACE17X_RS01795 (ACE17X_01795)	pilQ	407792..409888 (+)	2097	WP_010946666.1	type IV pilus secretin PilQ	Machinery gene
ACE17X_RS01800 (ACE17X_01800)	aroK	410334..410861 (+)	528	WP_010946667.1	shikimate kinase AroK	-

Sequence

Protein

• Length: 354 a.a.
• Molecular weight: 38732.71 Da
• Isoelectric Point: 5.0367

>NTDB_id=1052045 ACE17X_RS01775 WP_010946662.1 404975..406039(+) (pilM) [Legionella pneumophila strain lpg1386]
MLKLLKPKHRSILGIDISSSAVKILEISGSGEDICVEGYGREEIPASALEGNIIKDVDVVANCIKKLVERLKFTSKNVAL
AVPDSAVISKVVQINEGLTDLEMEELVVIEADKYIPYPIDEINLDFEVLGHSAKSSSLLDVLIVASRAENVNTRVEAAHR
AGLEPKVVDVESYAVERSVQQIAQELPASGQDKIVAIVDIGANYTHLFVLHGMRLIFSREEKFGGMQLVEAIAEHYHMNL
EQAIALKNQGKLPEDYESAVLEPFKDMILLQIKRTLQFFYSTSQHGFVDHILLAGGMARQSGLAALIQEQLGVTTTVANP
LSHMSFGKKVNLDVINSDAPTLMVACGLALRHVE

Nucleotide

• Length: 1065 bp

>NTDB_id=1052045 ACE17X_RS01775 WP_010946662.1 404975..406039(+) (pilM) [Legionella pneumophila strain lpg1386]
GTGCTCAAATTGCTTAAACCTAAGCATCGTTCAATTCTTGGCATTGACATCAGTTCTTCCGCAGTGAAAATCCTGGAAAT
ATCAGGTTCCGGAGAGGACATTTGTGTTGAAGGATATGGTCGCGAAGAAATACCTGCCTCTGCCTTAGAAGGAAATATAA
TTAAGGATGTCGATGTAGTAGCGAATTGTATCAAAAAATTAGTAGAAAGATTGAAGTTTACATCTAAAAATGTGGCATTG
GCTGTACCTGATTCGGCTGTTATCAGTAAAGTAGTGCAAATTAATGAAGGGTTAACTGACCTTGAAATGGAAGAGCTTGT
CGTTATTGAGGCGGATAAGTACATTCCTTACCCAATAGATGAAATAAACCTGGATTTTGAAGTGTTAGGCCATTCAGCCA
AGAGCTCATCACTGCTTGATGTTTTAATCGTGGCATCAAGGGCAGAAAACGTTAATACCCGTGTTGAAGCAGCCCATAGA
GCAGGGCTGGAACCCAAAGTTGTTGACGTTGAGTCTTATGCAGTAGAGCGTTCAGTACAACAAATCGCGCAAGAATTACC
AGCAAGTGGGCAAGACAAAATTGTTGCAATAGTAGATATTGGAGCCAATTATACTCACTTATTTGTTTTGCATGGTATGC
GACTAATCTTTTCAAGAGAAGAAAAATTTGGAGGTATGCAACTTGTTGAAGCTATTGCAGAACACTATCATATGAATTTG
GAGCAGGCTATTGCCCTAAAAAATCAGGGTAAACTACCGGAGGACTACGAATCTGCTGTATTAGAACCTTTTAAGGACAT
GATTCTTTTGCAGATCAAACGTACTTTGCAGTTTTTTTATTCAACCAGTCAACATGGATTTGTAGACCATATTTTATTGG
CGGGAGGAATGGCAAGGCAATCAGGATTGGCAGCGCTTATTCAAGAACAGTTAGGAGTGACTACTACAGTGGCAAATCCA
CTATCCCATATGTCTTTTGGTAAAAAAGTGAATTTGGATGTGATTAACAGTGATGCGCCTACACTGATGGTGGCTTGTGG
GTTGGCTTTGAGGCATGTCGAGTAA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-352)

3D structure

• AlphaFold DB: Q5ZX06

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Legionella pneumophila strain ERS1305867	99.435	100	0.994
comM	Acinetobacter nosocomialis M2	42.817	100	0.429
pilM	Acinetobacter baumannii D1279779	42.535	100	0.427
comM	Acinetobacter baylyi ADP1	41.457	100	0.418