Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   ACE1EH_RS11645 Genome accession   NZ_CP169535
Coordinates   2437236..2438306 (-) Length   356 a.a.
NCBI ID   WP_007612574.1    Uniprot ID   -
Organism   Bacillus velezensis strain HJ-16     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2432236..2443306
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACE1EH_RS11595 (ACE1EH_11595) sipW 2432295..2432879 (-) 585 WP_375004234.1 signal peptidase I SipW -
  ACE1EH_RS11600 (ACE1EH_11600) tapA 2432851..2433522 (-) 672 WP_375004235.1 amyloid fiber anchoring/assembly protein TapA -
  ACE1EH_RS11605 (ACE1EH_11605) - 2433781..2434110 (+) 330 WP_038459175.1 DUF3889 domain-containing protein -
  ACE1EH_RS11610 (ACE1EH_11610) - 2434151..2434330 (-) 180 WP_003153093.1 YqzE family protein -
  ACE1EH_RS11615 (ACE1EH_11615) comGG 2434387..2434764 (-) 378 WP_375004236.1 competence type IV pilus minor pilin ComGG Machinery gene
  ACE1EH_RS11620 (ACE1EH_11620) comGF 2434765..2435265 (-) 501 WP_375004237.1 competence type IV pilus minor pilin ComGF -
  ACE1EH_RS11625 (ACE1EH_11625) comGE 2435174..2435488 (-) 315 WP_326155219.1 competence type IV pilus minor pilin ComGE Machinery gene
  ACE1EH_RS11630 (ACE1EH_11630) comGD 2435472..2435909 (-) 438 WP_007612572.1 competence type IV pilus minor pilin ComGD Machinery gene
  ACE1EH_RS11635 (ACE1EH_11635) comGC 2435899..2436207 (-) 309 WP_003153087.1 competence type IV pilus major pilin ComGC Machinery gene
  ACE1EH_RS11640 (ACE1EH_11640) comGB 2436212..2437249 (-) 1038 WP_375004238.1 competence type IV pilus assembly protein ComGB Machinery gene
  ACE1EH_RS11645 (ACE1EH_11645) comGA 2437236..2438306 (-) 1071 WP_007612574.1 competence type IV pilus ATPase ComGA Machinery gene
  ACE1EH_RS11650 (ACE1EH_11650) - 2438503..2439453 (-) 951 WP_007408319.1 magnesium transporter CorA family protein -
  ACE1EH_RS11655 (ACE1EH_11655) - 2439599..2440900 (+) 1302 WP_375004239.1 hemolysin family protein -
  ACE1EH_RS11660 (ACE1EH_11660) - 2440938..2441768 (-) 831 WP_038459190.1 STAS domain-containing protein -
  ACE1EH_RS11670 (ACE1EH_11670) - 2441974..2442354 (-) 381 WP_003153079.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  ACE1EH_RS11675 (ACE1EH_11675) - 2442591..2442836 (+) 246 WP_003153078.1 DUF2626 domain-containing protein -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40530.84 Da        Isoelectric Point: 9.3437

>NTDB_id=1051877 ACE1EH_RS11645 WP_007612574.1 2437236..2438306(-) (comGA) [Bacillus velezensis strain HJ-16]
MKNIETYSRNIINEAYTARASDIHIVPREKDAFIHFRIGHALVKKRVLKKEECVRLISHFKFLSAMDIGERRKPQNGSLS
LPLPTETVHLRMSTLPTMNDESLVIRLLPKRQIPPLDKLSLFRGAGAALLSFLKHSHGLLLFTGPTGSGKTTTLYSLVNY
AKRHFNRRIVTLEDPVETRDEDVLQVQVNEKAGVTYSAGLKAILRHDPDMIILGEIRDAETAEIAVRAAMTGHLVLSSLH
TRDAKGAIYRLLEFGVNMTEIEQTVIAIAAQRLVDLTCPFCKEENCSVYCRMYRETRRAGIYELLYGKNLTQCFQEAKGE
HANFQYQTLRRLIRKGIALGYVTTDNYDRWVYHEAD

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=1051877 ACE1EH_RS11645 WP_007612574.1 2437236..2438306(-) (comGA) [Bacillus velezensis strain HJ-16]
GTGAAAAATATTGAAACATACAGCAGGAACATCATAAACGAGGCCTATACGGCAAGGGCTTCAGATATACACATTGTGCC
GAGAGAAAAGGATGCTTTCATTCATTTTCGCATCGGCCATGCTCTTGTGAAAAAAAGAGTCTTAAAAAAAGAGGAATGCG
TAAGGCTTATTTCTCATTTTAAGTTTTTATCGGCCATGGATATAGGAGAAAGACGTAAGCCGCAAAACGGGTCATTGTCT
CTGCCGCTTCCGACGGAAACCGTTCATTTGAGAATGTCAACCTTGCCGACAATGAATGATGAAAGTCTTGTCATCAGGCT
TTTGCCTAAGCGGCAGATTCCCCCTCTGGATAAATTGTCCTTATTTCGGGGGGCGGGCGCCGCATTATTGTCCTTTCTGA
AGCATTCGCACGGCCTGCTTCTGTTTACCGGTCCCACCGGATCTGGAAAAACGACCACTCTTTACTCGCTTGTCAACTAT
GCAAAAAGGCATTTCAACCGGCGGATTGTCACGCTTGAAGACCCCGTAGAAACAAGAGATGAGGATGTGTTGCAAGTTCA
GGTGAATGAAAAAGCCGGAGTCACATATTCCGCCGGTTTAAAAGCGATTTTAAGACATGATCCCGATATGATCATCTTAG
GGGAAATCAGGGATGCCGAGACGGCTGAAATCGCAGTCCGCGCGGCTATGACGGGACACCTCGTTCTTTCAAGCCTGCAT
ACAAGAGATGCTAAGGGCGCCATATACAGGCTGCTGGAGTTTGGCGTGAACATGACAGAAATTGAACAAACCGTCATTGC
CATTGCCGCTCAGCGTCTGGTTGATTTAACATGTCCGTTCTGCAAAGAGGAAAACTGTTCCGTGTATTGCCGGATGTACA
GGGAGACACGCCGTGCGGGCATTTATGAACTGCTCTACGGGAAAAACCTCACCCAATGCTTTCAGGAAGCAAAGGGAGAG
CACGCCAATTTTCAATATCAGACGCTCCGCAGGCTGATCAGAAAAGGAATCGCGCTCGGCTATGTCACGACCGACAATTA
CGATCGGTGGGTGTATCATGAAGCGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

82.022

100

0.82
  pilB Glaesserella parasuis strain SC1401

38.793

97.753

0.379
  pilF Thermus thermophilus HB27

38.177

98.596

0.376

Multiple sequence alignment