Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   ABJR11_RS06505 Genome accession   NZ_CP169354
Coordinates   1358357..1358836 (-) Length   159 a.a.
NCBI ID   WP_006248401.1    Uniprot ID   A0A249A0S4
Organism   Mannheimia haemolytica strain Mh184.20180323.PV.H     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1353357..1363836
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABJR11_RS06480 (ABJR11_001296) yacG 1354259..1354444 (-) 186 WP_006248406.1 DNA gyrase inhibitor YacG -
  ABJR11_RS06485 (ABJR11_001297) coaE 1354426..1355061 (-) 636 WP_020831305.1 dephospho-CoA kinase -
  ABJR11_RS06490 (ABJR11_001298) - 1355097..1355753 (-) 657 WP_006248404.1 prepilin peptidase -
  ABJR11_RS06495 (ABJR11_001299) pilC 1355756..1356952 (-) 1197 WP_006248403.1 type II secretion system F family protein Machinery gene
  ABJR11_RS06500 (ABJR11_001300) pilB 1356936..1358336 (-) 1401 WP_040080755.1 GspE/PulE family protein Machinery gene
  ABJR11_RS06505 (ABJR11_001301) pilA 1358357..1358836 (-) 480 WP_006248401.1 prepilin peptidase-dependent pilin Machinery gene
  ABJR11_RS06510 (ABJR11_001302) radA 1359017..1360396 (+) 1380 WP_040080753.1 DNA repair protein RadA Machinery gene
  ABJR11_RS06515 (ABJR11_001303) - 1360451..1360681 (-) 231 WP_006248398.1 YgjV family protein -
  ABJR11_RS06520 (ABJR11_001304) folA 1360801..1361295 (-) 495 WP_006248397.1 type 3 dihydrofolate reductase -
  ABJR11_RS06525 (ABJR11_001305) proB 1361407..1362498 (+) 1092 WP_006251346.1 glutamate 5-kinase -
  ABJR11_RS06530 (ABJR11_001306) - 1362498..1363262 (+) 765 WP_006248395.1 TSUP family transporter -

Sequence

Protein


Download         Length: 159 a.a.        Molecular weight: 16841.15 Da        Isoelectric Point: 8.7964

>NTDB_id=1050823 ABJR11_RS06505 WP_006248401.1 1358357..1358836(-) (pilA) [Mannheimia haemolytica strain Mh184.20180323.PV.H]
MQKIVHFRPLGIRQLHRAFTLIELMIVIAIIAILATIAIPSYNSYTQKAALSELLQASASYKSDVEVCIYNSGGLDNCSG
GTNGIQANKTSSNTTKYLNSIAVTKGVITVTGKGSLNGYGYTLTPSFSNNTISWTASCSGNDTSLFPANFCSTTVTPNG

Nucleotide


Download         Length: 480 bp        

>NTDB_id=1050823 ABJR11_RS06505 WP_006248401.1 1358357..1358836(-) (pilA) [Mannheimia haemolytica strain Mh184.20180323.PV.H]
ATGCAAAAAATTGTACATTTTAGACCGCTTGGCATTCGCCAACTGCATCGGGCATTTACCCTCATTGAGTTGATGATCGT
GATTGCGATTATTGCCATTCTTGCCACTATTGCGATCCCTTCCTATAACTCATACACCCAAAAGGCGGCGTTATCGGAGT
TATTACAAGCATCTGCTTCGTATAAATCAGACGTTGAAGTCTGTATTTACAACAGTGGCGGGTTGGATAACTGCTCCGGT
GGCACGAATGGGATTCAGGCGAATAAAACCAGTAGTAATACCACTAAATACCTAAACTCAATTGCGGTAACAAAAGGCGT
GATTACGGTAACCGGCAAAGGCTCTCTGAATGGTTATGGCTATACCTTAACACCGTCGTTCTCAAATAATACGATTAGTT
GGACGGCAAGTTGTAGTGGCAATGATACGAGCCTATTTCCAGCTAATTTCTGTAGCACAACTGTTACACCAAACGGCTAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(48-148)

Prokaryotic N-terminal methylation motif (N_methyl)

(16-38)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A249A0S4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Glaesserella parasuis strain SC1401

66.038

100

0.66
  pilA Haemophilus influenzae 86-028NP

56.429

88.05

0.497
  pilA Haemophilus influenzae Rd KW20

54.93

89.308

0.491

Multiple sequence alignment