Detailed information NTG1050484

Bioinformatically predicted

Overview

• Name: comFA
• Type: Machinery gene
• Locus tag: ACEY2B_RS06580
• Genome accession: NZ_CP169315
• Coordinates: 1357840..1359162 (+)
• Length: 440 a.a.
• NCBI ID: WP_136122464.1
• Uniprot ID: -
• Organism: Lactococcus lactis strain IFLLLBEK1
• Function: ssDNA transport into the cell (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1352840..1364162

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACEY2B_RS06555 (ACEY2B_06555)	glyQ	1353356..1354309 (-)	954	WP_003130667.1	glycine--tRNA ligase subunit alpha	-
ACEY2B_RS06560 (ACEY2B_06560)	-	1354595..1355341 (-)	747	WP_042230284.1	bifunctional metallophosphatase/5'-nucleotidase	-
ACEY2B_RS06565 (ACEY2B_06565)	-	1355456..1356475 (-)	1020	WP_136122465.1	DUF475 domain-containing protein	-
ACEY2B_RS06570 (ACEY2B_06570)	-	1356523..1357020 (-)	498	WP_015426305.1	VanZ family protein	-
ACEY2B_RS06575 (ACEY2B_06575)	-	1357154..1357783 (-)	630	WP_015426304.1	YigZ family protein	-
ACEY2B_RS06580 (ACEY2B_06580)	comFA	1357840..1359162 (+)	1323	WP_136122464.1	DEAD/DEAH box helicase	Machinery gene
ACEY2B_RS06585 (ACEY2B_06585)	comFC	1359405..1359809 (+)	405	WP_225513299.1	ComF family protein	Machinery gene
ACEY2B_RS06590 (ACEY2B_06590)	-	1359845..1360420 (-)	576	WP_015426301.1	nucleotidyltransferase family protein	-
ACEY2B_RS06595 (ACEY2B_06595)	-	1360434..1360889 (-)	456	WP_003130653.1	diacylglycerol kinase family protein	-
ACEY2B_RS06600 (ACEY2B_06600)	ybeY	1360873..1361361 (-)	489	WP_003130652.1	rRNA maturation RNase YbeY	-
ACEY2B_RS06605 (ACEY2B_06605)	-	1361513..1361992 (-)	480	WP_003130650.1	NUDIX hydrolase	-
ACEY2B_RS06610 (ACEY2B_06610)	-	1362050..1363018 (-)	969	WP_003130649.1	PhoH family protein	-

Sequence

Protein

• Length: 440 a.a.
• Molecular weight: 50083.49 Da
• Isoelectric Point: 10.1065

>NTDB_id=1050484 ACEY2B_RS06580 WP_136122464.1 1357840..1359162(+) (comFA) [Lactococcus lactis strain IFLLLBEK1]
MSTNQEKLFGRLLLKNDILQLIKSTDKISVSKIFSNFLLETTVKSILGMTSISSNKIKCNRCGTVHIKNSVKLPIGAFYC
PSCIQLGRVRSDEFLYFLPQKNFPKKSYINWSGKLTENQKSVSNALCQEINSHQQIIVQAVTGAGKTEMIYQVIEQILES
GGVVGLASPRIDVCLELHQRLSRDFSCKIPLLYHDGDSYFRAPLIIMTSHQLLRFKEAFDLLIIDEVDAFPFRDNEMLYF
AAEKARKVEGNLIYLTATSTDKLEKDIKKQKLYPLFLPRRFHNFPLVVPKFFWKNKFDKKLIEQRNSGFPLLIFAAEIES
GQEFAKQLQLKFPKEKIASVASTTKDRLEIVKAFRNKEITILIATSILERGVTFPNVDVFVINSEHPNFTKSALIQMAGR
VGRSPERPTGLVSFFHYGKSKAMCQAVREIKKMNQLGGFS

Nucleotide

• Length: 1323 bp

>NTDB_id=1050484 ACEY2B_RS06580 WP_136122464.1 1357840..1359162(+) (comFA) [Lactococcus lactis strain IFLLLBEK1]
ATGAGCACTAATCAAGAAAAGTTATTTGGCCGTTTATTATTAAAAAATGATATTTTACAACTTATAAAAAGTACTGACAA
GATTTCTGTCAGTAAAATTTTTAGTAATTTTTTGTTAGAAACAACGGTAAAATCAATTTTGGGAATGACTTCAATTTCTT
CCAATAAAATAAAATGCAACCGTTGTGGGACTGTTCATATAAAAAACTCTGTCAAACTTCCAATTGGTGCATTTTACTGT
CCAAGTTGTATTCAATTAGGTCGAGTCCGCTCGGATGAATTCTTGTACTTTCTGCCACAAAAGAATTTCCCAAAGAAATC
ATATATAAACTGGTCTGGAAAACTGACAGAGAATCAAAAATCAGTTTCAAATGCCCTCTGTCAGGAAATTAATTCTCATC
AGCAAATAATTGTCCAAGCTGTGACTGGAGCTGGAAAAACTGAAATGATTTATCAAGTCATTGAGCAAATTTTAGAAAGC
GGTGGAGTTGTTGGTCTAGCTAGTCCAAGAATTGATGTTTGTCTTGAACTTCATCAGCGATTATCACGTGATTTTTCCTG
TAAGATTCCACTCTTATATCATGATGGCGACAGCTATTTTCGAGCTCCATTAATAATAATGACCAGTCATCAGCTTTTAC
GTTTCAAGGAAGCTTTTGATTTGCTGATTATTGATGAGGTTGATGCCTTTCCCTTTAGAGATAATGAAATGCTTTATTTT
GCGGCAGAAAAAGCAAGAAAAGTAGAAGGAAATTTAATATATTTGACCGCAACTTCTACTGACAAACTTGAAAAAGATAT
AAAAAAGCAAAAACTCTATCCTTTGTTTCTCCCGCGTCGTTTTCACAATTTTCCTTTAGTGGTGCCTAAATTTTTTTGGA
AAAATAAATTTGATAAGAAATTAATTGAGCAAAGAAATAGTGGCTTTCCACTTCTAATTTTTGCTGCTGAAATTGAATCT
GGACAAGAATTTGCAAAACAACTACAACTAAAATTTCCTAAAGAAAAAATTGCTTCCGTTGCTTCAACAACAAAAGATAG
ATTGGAAATTGTTAAAGCTTTTAGAAATAAAGAAATTACTATTTTAATAGCGACTTCAATTCTTGAACGGGGAGTCACTT
TTCCAAATGTTGATGTTTTTGTCATCAACAGTGAGCACCCAAACTTCACTAAATCCGCACTGATACAAATGGCTGGACGT
GTGGGTCGTAGTCCTGAACGTCCAACAGGCTTAGTTAGTTTTTTTCATTACGGAAAATCCAAAGCAATGTGTCAGGCAGT
TAGAGAAATCAAAAAAATGAACCAACTGGGAGGTTTTTCATGA

Domains

Predicted by InterproScan.

• Type III restriction enzyme, res subunit (ResIII) (114-228)

• Helicase conserved C-terminal domain (Helicase_C) (300-403)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comFA	Lactococcus lactis subsp. cremoris KW2	74	90.909	0.673
comFA/cflA	Streptococcus mitis SK321	52.513	90.455	0.475
comFA/cflA	Streptococcus pneumoniae Rx1	52.513	90.455	0.475
comFA/cflA	Streptococcus pneumoniae D39	52.513	90.455	0.475
comFA/cflA	Streptococcus pneumoniae R6	52.513	90.455	0.475
comFA/cflA	Streptococcus pneumoniae TIGR4	52.513	90.455	0.475
comFA/cflA	Streptococcus mitis NCTC 12261	51.508	90.455	0.466
comFA	Bacillus subtilis subsp. subtilis str. 168	37.472	100	0.384