Detailed information NTG1050329

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: ACEYW7_RS15620
• Genome accession: NZ_CP169298
• Coordinates: 3251761..3252879 (+)
• Length: 372 a.a.
• NCBI ID: WP_000347041.1
• Uniprot ID: A0A3R9E3W9
• Organism: Acinetobacter baumannii strain AMA205
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3246761..3257879

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACEYW7_RS15610 (ACEYW7_15610)	-	3249877..3250569 (-)	693	WP_001108516.1	YggS family pyridoxal phosphate-dependent enzyme	-
ACEYW7_RS15615 (ACEYW7_15615)	pilT	3250696..3251733 (+)	1038	WP_000355489.1	type IV pilus twitching motility protein PilT	Machinery gene
ACEYW7_RS15620 (ACEYW7_15620)	pilU	3251761..3252879 (+)	1119	WP_000347041.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
ACEYW7_RS15625 (ACEYW7_15625)	fur	3252949..3253386 (-)	438	WP_001122847.1	ferric iron uptake transcriptional regulator	-
ACEYW7_RS15630 (ACEYW7_15630)	-	3253498..3253896 (+)	399	WP_001170994.1	outer membrane protein assembly factor BamE	-
ACEYW7_RS15635 (ACEYW7_15635)	-	3253951..3254274 (-)	324	WP_000442585.1	RnfH family protein	-
ACEYW7_RS15640 (ACEYW7_15640)	-	3254274..3255341 (-)	1068	WP_029642282.1	membrane protein	-
ACEYW7_RS15645 (ACEYW7_15645)	-	3255494..3255949 (-)	456	WP_000782976.1	bacteriohemerythrin	-
ACEYW7_RS15650 (ACEYW7_15650)	-	3256202..3257050 (-)	849	WP_000336554.1	class II glutamine amidotransferase	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41922.14 Da
• Isoelectric Point: 6.5789

>NTDB_id=1050329 ACEYW7_RS15620 WP_000347041.1 3251761..3252879(+) (pilU) [Acinetobacter baumannii strain AMA205]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLQSIMSEKQRKEFAETRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLADEGPDQLSDGKQYLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=1050329 ACEYW7_RS15620 WP_000347041.1 3251761..3252879(+) (pilU) [Acinetobacter baumannii strain AMA205]
ATGGATTTTAATGACTTGCTCAATCTGATGGTTGAAAAAAAATCATCCGACCTCTTTATTACAGATGGCGTTGCGCCCTC
TATGAAGATTAACGGGCAAATTGTTCCAATTTCAAAAAATAGTCTTTCTGGGGAAGTGATTGGTCAACTGTTACAGTCCA
TCATGAGTGAAAAACAACGCAAAGAATTTGCAGAAACTCGTGAATGTAACTTTGCCATTATGAACCGTGAAAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAGCGCGACATGCCGGGCATGGTACTACGTCGTATTGAAACCAAGATTCCTTCAAT
TGATGACTTGCAGTTACCGCCTGTACTTAAAGATTTATCGATGACCAAACGCGGCATTATTATTTTCGTAGGCGCGACAG
GTACAGGTAAATCCACTTCATTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAGGGCCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCACGAACATGCAGGTTGCATCATTACTCAGCGTGAAGTCGGGATCGATACCGACTCATTTGA
AATTGCCTTGAAAAATACACTACGACAAGCGCCGGATGTGATCTTAATTGGTGAGATCCGTTCTCGTGAAGTCATGGACT
ATGCGATTGGCTTTGCTGAAACAGGTCACCTTGTATTAGCTACCATGCACGCTAACAACGCCAACCAAGCGCTCGACCGT
ATTATTCACTTTTTTGAAAGTGATCGTCATAGTCAGCTTTACATGGACTTGTCACTTAACTTAAAGGCTATGATTGCGCA
GCAGCTCATTCCAACACCAGATGGTAATTCACGCCGTGCAGCAATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAAGATTTGATGAAACGCTCACGTGAACTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATATAAAGCTGGTCAAATTACCTACAAAGATGCACTTAAACATGCTGACTCACCGAACGATTTACG
TTTAACCATTAAACTTGCTGATGAAGGTCCCGATCAATTATCAGACGGGAAACAGTATTTAACTTTTGACCGCCAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (29-270)

3D structure

• AlphaFold DB: A0A3R9E3W9

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.926	100	0.772
pilU	Pseudomonas stutzeri DSM 10701	68.194	99.731	0.68
pilU	Vibrio cholerae strain A1552	52.973	99.462	0.527
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.197	93.011	0.392