Detailed information    

insolico Bioinformatically predicted

Overview

Name   comE   Type   Machinery gene
Locus tag   ACEVB2_RS03390 Genome accession   NZ_CP169223
Coordinates   700148..701482 (-) Length   444 a.a.
NCBI ID   WP_374091797.1    Uniprot ID   -
Organism   Pasteurella multocida subsp. gallicida strain W24_252     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 695148..706482
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACEVB2_RS03365 (ACEVB2_03365) secM 696668..696979 (-) 312 WP_005723689.1 secA translation cis-regulator SecM -
  ACEVB2_RS03370 (ACEVB2_03370) - 697072..697377 (+) 306 WP_005723691.1 DUF721 domain-containing protein -
  ACEVB2_RS03375 (ACEVB2_03375) - 697424..698329 (-) 906 WP_005723696.1 Dam family site-specific DNA-(adenine-N6)-methyltransferase -
  ACEVB2_RS03380 (ACEVB2_03380) aroB 698333..699421 (-) 1089 WP_374091796.1 3-dehydroquinate synthase -
  ACEVB2_RS03385 (ACEVB2_03385) aroK 699438..699965 (-) 528 WP_005717623.1 shikimate kinase AroK -
  ACEVB2_RS03390 (ACEVB2_03390) comE 700148..701482 (-) 1335 WP_374091797.1 type IV pilus secretin PilQ Machinery gene
  ACEVB2_RS03395 (ACEVB2_03395) - 701492..701854 (-) 363 WP_374091798.1 pilus assembly protein PilP -
  ACEVB2_RS03400 (ACEVB2_03400) - 701857..702381 (-) 525 WP_005751903.1 hypothetical protein -
  ACEVB2_RS03405 (ACEVB2_03405) - 702381..702896 (-) 516 WP_374091799.1 competence protein ComB -
  ACEVB2_RS03410 (ACEVB2_03410) - 702936..703709 (-) 774 WP_014668624.1 pilus assembly protein PilM -
  ACEVB2_RS03415 (ACEVB2_03415) - 703845..706406 (+) 2562 WP_005754757.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 444 a.a.        Molecular weight: 49300.74 Da        Isoelectric Point: 8.4449

>NTDB_id=1049896 ACEVB2_RS03390 WP_374091797.1 700148..701482(-) (comE) [Pasteurella multocida subsp. gallicida strain W24_252]
MWRAFRKISFVYFLCGVAYVGSSQAQDAEHFYLRLKQAPLVEMLQYLALQQHQDLLIDDHLESTLSLQMKKTTFEKCLRS
IAKMKQLELHQEGKSYYLTSPSSVAANDTHHPTSLMTSSIKLHFAKAAEVVKSLTSGQGSLLSVGGSLSFDERTNLLLIQ
DEPQSIQRIKALVAEMDKPIEQIAIEARIVTMTDESLQELGVRWGLFQATEQAHTIAGSLAANGFSNIENQLNVNFSTNS
APVGSIALQLAKINGRLLDLELTALEREKHIEIIASPRLLTTNKKSASIKQGTEIPYVMKRGKDKSESVEFREAVLGLDV
TPHISKDNTILLDLLITQNTLGAPVVYDKGEIVSIDKQEINTQVVAQDGETIVLGGVFHDTMTKGVNKVPLLGDLPLLKH
VFSQKTERHQKRELVIFVTPHIIKPSQGSPEQKTTRVKKSAKSR

Nucleotide


Download         Length: 1335 bp        

>NTDB_id=1049896 ACEVB2_RS03390 WP_374091797.1 700148..701482(-) (comE) [Pasteurella multocida subsp. gallicida strain W24_252]
ATGTGGCGAGCATTCAGAAAAATATCTTTTGTGTACTTTTTATGTGGAGTTGCTTATGTTGGAAGTAGTCAAGCACAAGA
CGCAGAACATTTTTATTTACGTTTAAAACAAGCGCCTTTAGTCGAAATGTTACAGTATTTAGCATTGCAACAACATCAGG
ATTTGTTAATCGATGATCATTTAGAGAGCACATTATCATTACAGATGAAAAAGACAACCTTTGAGAAATGTTTACGGTCG
ATTGCAAAAATGAAACAACTTGAGTTACATCAAGAAGGAAAATCCTATTATTTAACTTCCCCTTCAAGTGTTGCAGCAAA
CGATACTCATCATCCTACGTCATTGATGACATCTTCAATAAAATTGCATTTTGCCAAAGCTGCAGAGGTAGTGAAATCTT
TAACTTCAGGGCAGGGAAGTTTACTTTCGGTCGGGGGGAGTTTGAGTTTTGATGAGCGGACTAATTTACTGCTGATTCAG
GATGAACCGCAATCAATACAGCGTATTAAAGCATTAGTAGCAGAAATGGATAAACCCATTGAACAAATTGCGATCGAAGC
TAGGATTGTGACGATGACAGACGAAAGTTTGCAGGAACTTGGTGTAAGATGGGGGCTATTTCAAGCAACAGAACAGGCAC
ATACTATTGCAGGGAGTTTAGCCGCGAACGGCTTTTCGAATATAGAAAACCAATTAAATGTGAATTTCTCGACCAATAGT
GCACCTGTTGGTTCCATCGCCTTACAGTTGGCGAAAATAAATGGTCGATTATTAGACTTGGAATTAACTGCCTTGGAGCG
AGAAAAGCATATTGAGATTATTGCGAGTCCTCGTTTATTAACAACGAATAAAAAAAGTGCCAGTATCAAACAAGGGACGG
AAATTCCTTATGTGATGAAGCGGGGAAAAGATAAAAGCGAATCGGTGGAATTTCGAGAAGCTGTATTGGGTTTAGATGTG
ACACCGCATATCTCAAAAGACAACACGATTTTATTAGATTTATTGATTACACAAAATACATTAGGTGCACCGGTAGTGTA
TGATAAAGGCGAAATTGTTTCGATCGATAAACAGGAAATCAATACTCAAGTCGTCGCTCAAGATGGTGAAACCATCGTTT
TAGGTGGGGTGTTTCATGATACGATGACAAAGGGAGTCAATAAAGTACCACTACTAGGGGATTTGCCTTTGCTTAAACAT
GTGTTTAGCCAGAAAACTGAACGTCATCAAAAGCGGGAATTAGTGATTTTTGTCACTCCTCATATTATCAAACCTAGCCA
AGGTTCGCCTGAACAAAAAACAACAAGAGTTAAAAAATCTGCAAAATCGAGGTGA

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(264-424)

Bacterial type II/III secretion system short domain (Secretin_N)

(119-182)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comE Haemophilus influenzae Rd KW20

61.574

97.297

0.599
  comE Haemophilus influenzae 86-028NP

60.88

97.297

0.592
  comE Glaesserella parasuis strain SC1401

49.192

97.523

0.48
  pilQ Vibrio campbellii strain DS40M4

39.286

94.595

0.372
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

39.563

92.793

0.367
  pilQ Vibrio cholerae strain A1552

39.563

92.793

0.367

Multiple sequence alignment