Detailed information NTG1049848

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: ACEPPP_RS02865
• Genome accession: NZ_CP169217
• Coordinates: 652700..653761 (-)
• Length: 353 a.a.
• NCBI ID: WP_374089554.1
• Uniprot ID: -
• Organism: Methylomicrobium lacus strain 22M6SE5-12
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 647700..658761

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACEPPP_RS02840 (ACEPPP_02840)	aroK	647907..648437 (-)	531	WP_374089550.1	shikimate kinase AroK	-
ACEPPP_RS02845 (ACEPPP_02845)	pilQ	648652..650934 (-)	2283	WP_374089551.1	type IV pilus secretin PilQ	-
ACEPPP_RS02850 (ACEPPP_02850)	-	650951..651466 (-)	516	WP_374090869.1	pilus assembly protein PilP	-
ACEPPP_RS02855 (ACEPPP_02855)	-	651511..652140 (-)	630	WP_374089552.1	type 4a pilus biogenesis protein PilO	-
ACEPPP_RS02860 (ACEPPP_02860)	-	652137..652700 (-)	564	WP_374089553.1	PilN domain-containing protein	-
ACEPPP_RS02865 (ACEPPP_02865)	pilM	652700..653761 (-)	1062	WP_374089554.1	pilus assembly protein PilM	Machinery gene
ACEPPP_RS02870 (ACEPPP_02870)	rpmE	654642..654842 (-)	201	WP_374089555.1	50S ribosomal protein L31	-
ACEPPP_RS02875 (ACEPPP_02875)	-	654936..656288 (-)	1353	WP_374089556.1	MFS transporter	-
ACEPPP_RS02880 (ACEPPP_02880)	nhaD	656341..657648 (-)	1308	WP_374090870.1	sodium:proton antiporter NhaD	-

Sequence

Protein

• Length: 353 a.a.
• Molecular weight: 37895.29 Da
• Isoelectric Point: 4.6512

>NTDB_id=1049848 ACEPPP_RS02865 WP_374089554.1 652700..653761(-) (pilM) [Methylomicrobium lacus strain 22M6SE5-12]
MNWFNQKQSAVLGIDISTAAIKLLELSKSGSRYRVESYAVTPLPKDAIVDKNIANADVISAAIKAAIKQSGTKLKQAAVA
VSGASVMTKIITMPASLSDAEMEEQIMMEADQYVPYSLDDVNFDFEVQRVNESNPELVDVLLAASRKENVDDRVGALNQA
GLKAKIVDVEAFAIENAFALLADQFEESVKGKTVAIADIGASVVALNVLQDGRTVYTREQAFGGKQLTEEIQRRYGLSYE
EAGLAKKQGGLPDSYAVDVLEPFKKAMVQQIARSLQFFVSSSANRGIDSIILAGGCASITGVDKLVEQSLGVPAYIANPF
VNMSLSNRVRPQGLSNDAPAMMIACGLALRSFD

Nucleotide

• Length: 1062 bp

>NTDB_id=1049848 ACEPPP_RS02865 WP_374089554.1 652700..653761(-) (pilM) [Methylomicrobium lacus strain 22M6SE5-12]
ATGAACTGGTTTAATCAGAAACAGAGTGCGGTTCTTGGCATCGATATCAGTACGGCGGCGATAAAGTTGCTGGAACTGAG
CAAATCGGGCTCGCGTTACCGGGTTGAGAGCTATGCGGTGACCCCTTTGCCGAAAGACGCGATCGTCGATAAAAATATCG
CCAATGCGGATGTTATCTCCGCTGCGATTAAGGCGGCGATCAAACAGTCCGGAACCAAGCTGAAACAAGCGGCGGTGGCG
GTTTCCGGTGCCTCTGTGATGACTAAAATCATCACGATGCCGGCTTCATTGTCCGATGCGGAAATGGAGGAGCAGATCAT
GATGGAAGCCGATCAGTATGTGCCGTATTCGCTGGATGACGTTAATTTCGATTTCGAAGTGCAAAGAGTCAATGAAAGCA
ATCCCGAATTGGTCGACGTGCTGCTGGCGGCATCGAGAAAAGAAAATGTCGATGATCGCGTCGGCGCCTTAAATCAGGCT
GGCCTGAAGGCAAAGATTGTCGATGTGGAAGCTTTCGCAATTGAAAATGCGTTTGCCTTGCTTGCCGATCAGTTTGAGGA
GTCGGTGAAAGGGAAAACGGTCGCGATCGCGGACATCGGGGCTTCGGTCGTGGCGCTCAATGTCTTGCAGGATGGACGCA
CGGTATATACCCGTGAACAGGCTTTCGGCGGCAAGCAACTCACCGAGGAAATTCAACGCCGCTACGGCCTTTCCTATGAA
GAAGCGGGATTGGCGAAAAAACAGGGGGGATTGCCGGACAGCTATGCGGTTGACGTGCTGGAGCCCTTTAAAAAGGCGAT
GGTGCAGCAAATTGCCCGGTCGTTGCAGTTTTTCGTATCGTCCAGCGCCAACCGCGGCATAGACAGCATCATTCTTGCGG
GGGGATGTGCCTCGATTACCGGCGTGGACAAATTGGTTGAACAAAGTCTCGGTGTGCCGGCTTATATCGCCAATCCGTTC
GTGAACATGTCGCTGTCGAACCGGGTAAGGCCCCAGGGATTGAGTAACGACGCGCCTGCGATGATGATCGCATGCGGCCT
GGCTTTGAGGAGTTTCGACTAA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (13-352)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	49.008	100	0.49
comM	Acinetobacter nosocomialis M2	49.008	100	0.49
comM	Acinetobacter baylyi ADP1	48.159	100	0.482
pilM	Legionella pneumophila strain ERS1305867	41.643	100	0.416