Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   ACEPA4_RS16130 Genome accession   NZ_CP169216
Coordinates   3492926..3493984 (-) Length   352 a.a.
NCBI ID   WP_014038321.1    Uniprot ID   A0AAP7J1E4
Organism   Stenotrophomonas sp. P2112     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3487926..3498984
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACEPA4_RS16110 (ACEPA4_16110) - 3488864..3490834 (-) 1971 WP_065185316.1 type IV pilus secretin PilQ -
  ACEPA4_RS16115 (ACEPA4_16115) - 3490856..3491389 (-) 534 WP_005418914.1 pilus assembly protein PilP -
  ACEPA4_RS16120 (ACEPA4_16120) - 3491389..3492045 (-) 657 WP_057493352.1 type 4a pilus biogenesis protein PilO -
  ACEPA4_RS16125 (ACEPA4_16125) - 3492042..3492926 (-) 885 WP_065185317.1 PilN domain-containing protein -
  ACEPA4_RS16130 (ACEPA4_16130) pilM 3492926..3493984 (-) 1059 WP_014038321.1 pilus assembly protein PilM Machinery gene
  ACEPA4_RS16135 (ACEPA4_16135) - 3494204..3496627 (+) 2424 WP_111128205.1 penicillin-binding protein 1A -
  ACEPA4_RS16140 (ACEPA4_16140) - 3496817..3497461 (+) 645 WP_049411389.1 hypothetical protein -
  ACEPA4_RS16145 (ACEPA4_16145) - 3497690..3498436 (-) 747 WP_374082892.1 hypothetical protein -

Sequence

Protein


Download         Length: 352 a.a.        Molecular weight: 37741.35 Da        Isoelectric Point: 4.4006

>NTDB_id=1049840 ACEPA4_RS16130 WP_014038321.1 3492926..3493984(-) (pilM) [Stenotrophomonas sp. P2112]
MGLIPKSQSPLVGVDISSTAVKLLQLSRSGNRFRVEHYAVEPLPPNAVVEKNIVEVEAVGEAIRRAMNRSGSKAKLAAAA
VAGSAVITKVIPMPADLDENDMEAQIELEAVNYIPYPIEEVNLDFEVIGSIPNNPEMVQVLLAASRSENVELRQSALELG
GLQAKVMDVEAFAVENAYALVASELPVSIDGVVALVDIGATMTTLNVLRGGRSLYSREQVFGGKQLTDEIMRRYGLSYEE
AGLAKRQGGLPESYEMEVLEPFKEATVQQISRLLQFFYAGSEFNRVDHIVLAGGCAVLGGLPEMVEEQLGVPTVVANPLA
QMTLGPKVNAHALAQDAPALMIATGLALRSFD

Nucleotide


Download         Length: 1059 bp        

>NTDB_id=1049840 ACEPA4_RS16130 WP_014038321.1 3492926..3493984(-) (pilM) [Stenotrophomonas sp. P2112]
GTGGGGCTCATCCCAAAAAGTCAGTCGCCGCTTGTAGGCGTCGACATCAGTTCGACTGCGGTAAAGCTTTTGCAGCTGTC
CCGCAGCGGCAATCGTTTCCGCGTGGAACATTACGCTGTGGAACCTCTTCCGCCGAATGCGGTGGTGGAGAAGAACATCG
TCGAAGTGGAGGCCGTCGGTGAGGCCATCCGCCGGGCGATGAACCGTTCAGGCAGCAAGGCCAAGTTGGCCGCTGCCGCC
GTCGCGGGGTCGGCGGTGATCACCAAGGTGATCCCGATGCCGGCCGATCTCGACGAAAACGACATGGAAGCCCAGATCGA
GCTGGAAGCGGTCAACTACATTCCGTATCCGATCGAGGAAGTGAACCTGGACTTCGAGGTGATCGGGTCGATCCCGAACA
ACCCGGAGATGGTCCAGGTGCTGCTGGCCGCGTCGCGTTCGGAGAATGTCGAGCTGCGCCAGTCCGCGCTGGAGCTCGGT
GGCCTGCAGGCCAAGGTGATGGACGTGGAGGCCTTTGCGGTCGAGAACGCCTACGCCCTGGTCGCCAGCGAGCTGCCGGT
GTCCATCGACGGCGTGGTTGCGCTGGTCGACATCGGCGCCACCATGACCACCCTCAATGTCCTGCGCGGCGGTCGTAGCC
TTTACAGCCGCGAGCAGGTGTTCGGTGGCAAGCAGCTGACCGACGAGATCATGCGCCGCTACGGCCTGAGCTACGAGGAA
GCCGGGCTGGCCAAGCGCCAGGGCGGCCTGCCGGAAAGCTACGAGATGGAGGTGCTGGAGCCGTTCAAGGAAGCCACGGT
CCAGCAGATCAGCCGCCTGCTGCAGTTCTTCTATGCGGGCAGCGAATTCAACCGCGTCGATCACATCGTGCTGGCCGGCG
GCTGCGCCGTGCTCGGTGGCCTGCCGGAGATGGTCGAGGAACAGCTGGGTGTGCCGACCGTGGTCGCCAACCCGCTGGCG
CAGATGACCCTGGGGCCGAAGGTGAACGCGCATGCGTTGGCCCAGGATGCCCCCGCGCTGATGATCGCGACCGGTCTGGC
GCTGAGGAGCTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(13-351)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

51.42

100

0.514
  comM Acinetobacter nosocomialis M2

51.42

100

0.514
  comM Acinetobacter baylyi ADP1

50.284

100

0.503
  pilM Legionella pneumophila strain ERS1305867

47.863

99.716

0.477

Multiple sequence alignment