Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ACEPA4_RS04870 Genome accession   NZ_CP169216
Coordinates   1046487..1047629 (+) Length   380 a.a.
NCBI ID   WP_005415539.1    Uniprot ID   A0A246HZM3
Organism   Stenotrophomonas sp. P2112     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1041487..1052629
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACEPA4_RS04840 (ACEPA4_04840) soxR 1041596..1042030 (+) 435 WP_005415534.1 redox-sensitive transcriptional activator SoxR -
  ACEPA4_RS04845 (ACEPA4_04845) - 1042109..1042711 (-) 603 WP_005415535.1 short chain dehydrogenase -
  ACEPA4_RS04850 (ACEPA4_04850) - 1042810..1043685 (+) 876 WP_095377013.1 LysR family transcriptional regulator -
  ACEPA4_RS04855 (ACEPA4_04855) proC 1043746..1044567 (-) 822 WP_065177953.1 pyrroline-5-carboxylate reductase -
  ACEPA4_RS04860 (ACEPA4_04860) - 1044586..1045263 (-) 678 WP_262090362.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACEPA4_RS04865 (ACEPA4_04865) pilT 1045351..1046388 (+) 1038 WP_004147099.1 type IV pilus twitching motility protein PilT Machinery gene
  ACEPA4_RS04870 (ACEPA4_04870) pilU 1046487..1047629 (+) 1143 WP_005415539.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACEPA4_RS04875 (ACEPA4_04875) - 1047751..1048734 (+) 984 WP_065185821.1 aldo/keto reductase -
  ACEPA4_RS04880 (ACEPA4_04880) - 1048770..1049669 (-) 900 WP_065177951.1 LysR family transcriptional regulator -
  ACEPA4_RS04885 (ACEPA4_04885) - 1049982..1050194 (+) 213 WP_065185822.1 DUF4287 domain-containing protein -
  ACEPA4_RS04890 (ACEPA4_04890) - 1050303..1051010 (+) 708 WP_005415543.1 YitT family protein -
  ACEPA4_RS04895 (ACEPA4_04895) - 1051093..1051638 (-) 546 WP_005415544.1 DNA-3-methyladenine glycosylase I -

Sequence

Protein


Download         Length: 380 a.a.        Molecular weight: 42323.63 Da        Isoelectric Point: 6.7717

>NTDB_id=1049829 ACEPA4_RS04870 WP_005415539.1 1046487..1047629(+) (pilU) [Stenotrophomonas sp. P2112]
MNTTATTIDFTSFLKLMAHQRASDLFITAGMPPAMKVNGKISPITQTPLTPQQSRDLVLNVMTPAQREEFEKTHECNFAI
GLSGVGRFRVSCFYQRNQVGMVLRRIETRIPTVEELSLPPIIKTLAMTKRGIILFVGATGTGKSTSLAAMIGYRNQNSTG
HIITIEDPIEFVHKHEGCIITQREVGIDTDSWEAALKNTLRQAPDVIMIGEVRTREGMDHAIAFAETGHLVLATLHANNA
NQAMDRIVNFFPEDRRNQLLMDLSLNLKGVVAQQLVPSPDGRSRKVAMEILLGTPLVQDYIRDGEIHKLKEVMKDSVQLG
MKTFDQSLFELYQAGEISYEDALRYADSQNEVRLRIKLSQGGDARTLSQGLDGVEISEIR

Nucleotide


Download         Length: 1143 bp        

>NTDB_id=1049829 ACEPA4_RS04870 WP_005415539.1 1046487..1047629(+) (pilU) [Stenotrophomonas sp. P2112]
GTGAACACCACCGCGACCACCATCGATTTCACTTCGTTCCTCAAGCTGATGGCGCACCAGCGCGCCTCTGACCTGTTCAT
CACTGCGGGTATGCCACCGGCGATGAAGGTCAACGGCAAGATCTCGCCGATCACGCAGACGCCACTGACGCCGCAGCAGA
GCCGCGACCTGGTCCTCAACGTGATGACCCCGGCACAGCGCGAGGAATTCGAGAAGACCCACGAATGCAACTTCGCCATC
GGCCTGTCCGGTGTCGGGCGCTTCCGTGTCAGCTGCTTCTACCAGCGCAACCAGGTCGGCATGGTGCTGCGTCGCATCGA
GACGCGCATTCCGACCGTGGAAGAGCTGAGCCTACCGCCGATCATCAAGACGCTGGCGATGACCAAGCGCGGCATCATCC
TGTTCGTCGGTGCCACCGGTACCGGTAAATCGACTTCGCTGGCGGCGATGATCGGTTACCGCAACCAGAATTCGACCGGC
CACATCATCACCATCGAAGACCCGATCGAATTCGTGCACAAGCACGAGGGCTGCATCATCACCCAGCGCGAAGTCGGCAT
CGATACCGACAGCTGGGAAGCTGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGTA
CCCGCGAAGGCATGGACCACGCCATCGCGTTCGCCGAAACCGGCCACCTGGTGCTGGCCACGTTGCATGCCAACAATGCC
AACCAGGCGATGGACCGCATCGTCAACTTCTTCCCGGAAGACCGCCGCAACCAGCTGTTGATGGACCTGTCGCTGAACCT
CAAGGGCGTGGTCGCGCAGCAGCTGGTGCCGTCGCCCGATGGCCGCTCGCGCAAGGTGGCGATGGAGATCCTGCTGGGCA
CGCCACTGGTACAGGACTACATCCGCGACGGCGAGATCCACAAGTTGAAGGAAGTGATGAAGGACTCGGTCCAGCTGGGC
ATGAAGACCTTCGACCAGAGCCTGTTCGAGCTGTACCAGGCCGGCGAGATCAGCTACGAGGACGCGCTGCGTTACGCCGA
TTCGCAGAACGAGGTGCGCCTGCGCATCAAGCTCAGCCAGGGCGGCGACGCGCGCACCCTGTCGCAGGGGCTGGATGGAG
TGGAAATCTCCGAAATCCGCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(122-277)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A246HZM3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

68.12

96.579

0.658
  pilU Acinetobacter baylyi ADP1

63.866

93.947

0.6
  pilU Vibrio cholerae strain A1552

53.652

93.684

0.503
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.099

93.421

0.403

Multiple sequence alignment