Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACEJ41_RS10725 Genome accession   NZ_CP169056
Coordinates   2339608..2341338 (-) Length   576 a.a.
NCBI ID   WP_374012262.1    Uniprot ID   -
Organism   Pseudoxanthomonas koreensis strain TDY-1     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2334608..2346338
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACEJ41_RS10700 (ACEJ41_10700) - 2335381..2336364 (-) 984 WP_374012258.1 TonB C-terminal domain-containing protein -
  ACEJ41_RS10705 (ACEJ41_10705) tolR 2336374..2336808 (-) 435 WP_374012259.1 protein TolR -
  ACEJ41_RS10710 (ACEJ41_10710) tolQ 2336846..2337625 (-) 780 WP_374012260.1 protein TolQ -
  ACEJ41_RS10715 (ACEJ41_10715) ybgC 2337721..2338110 (-) 390 Protein_2097 tol-pal system-associated acyl-CoA thioesterase -
  ACEJ41_RS10720 (ACEJ41_10720) - 2338672..2339277 (-) 606 WP_374012261.1 transposase -
  ACEJ41_RS10725 (ACEJ41_10725) pilB 2339608..2341338 (-) 1731 WP_374012262.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACEJ41_RS10730 (ACEJ41_10730) - 2341466..2343385 (-) 1920 WP_374012263.1 sensor histidine kinase -
  ACEJ41_RS10735 (ACEJ41_10735) - 2343388..2345613 (-) 2226 WP_374012264.1 EAL domain-containing protein -

Sequence

Protein


Download         Length: 576 a.a.        Molecular weight: 62410.62 Da        Isoelectric Point: 5.4442

>NTDB_id=1049604 ACEJ41_RS10725 WP_374012262.1 2339608..2341338(-) (pilB) [Pseudoxanthomonas koreensis strain TDY-1]
MNAAVSANLVGITGIARRLVQDGAMDEAAARAAMAQASEAKVPLAQWIADKKLVGAAQLAAANAIEFGMPLLDASTFDPA
QNAMKLVSDELVQKHHVLPLFKRGGKLFVGTSNPTQTAALAELQFHTNLVVEPILVDDDQIRRTLEQWQNAGDQFSGALG
DDDDDGMDSLEVGTDEDAGSDSGVDAKGDDTPVVKFVNKALVDAIRRGASDIHFEPYETDYRVRVRIDGLLKTVARAPVK
LSQRIAARLKVMAQLDIAEKRVPQDGRIKLNLSKTKQIDFRVSTLPTLFGEKVVLRILDGSAAKLGIDKLGYEPDQQKLF
MDAIQKPYGMVLVTGPTGSGKTVSLYTALGILNDDLRNISTVEDPVEIRLPGVNQVQQNVKRGMTFAAALRSFLRQDPDI
IMVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDGPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCSKCKKAVVLPEH
ALLAEGFTEDEIKTGFTVYEAVGCEDCTEGYKGRTGIYQVMPMSEEIQAIVLEGGNAMQIAEVATRAGINDLRRSALLKV
RQGIISLAEINRVTKD

Nucleotide


Download         Length: 1731 bp        

>NTDB_id=1049604 ACEJ41_RS10725 WP_374012262.1 2339608..2341338(-) (pilB) [Pseudoxanthomonas koreensis strain TDY-1]
ATGAACGCTGCGGTATCCGCCAATCTGGTTGGCATCACCGGCATTGCCCGGCGCCTTGTGCAGGACGGCGCCATGGACGA
GGCGGCGGCGCGTGCCGCCATGGCCCAGGCTTCCGAGGCCAAGGTGCCCCTGGCCCAGTGGATCGCCGACAAGAAGCTGG
TGGGCGCCGCGCAACTGGCCGCGGCCAACGCGATCGAGTTCGGCATGCCGTTGCTGGATGCTTCCACGTTCGATCCGGCC
CAGAACGCGATGAAGCTGGTCAGCGACGAGCTGGTGCAGAAGCACCACGTGCTGCCCTTGTTCAAGCGCGGCGGCAAGTT
GTTCGTGGGCACCAGCAACCCGACCCAGACCGCCGCCCTGGCCGAACTCCAGTTCCACACCAACCTGGTGGTCGAACCGA
TCCTGGTCGACGATGACCAGATCCGCCGCACCCTGGAGCAGTGGCAGAACGCCGGCGACCAGTTCTCCGGCGCGCTGGGT
GACGACGATGACGACGGCATGGATTCGCTGGAGGTGGGCACCGACGAAGACGCCGGTTCCGACTCTGGCGTGGATGCCAA
GGGCGACGACACCCCGGTGGTGAAGTTCGTCAACAAGGCGCTGGTGGACGCCATCCGCCGCGGCGCCTCGGACATCCACT
TCGAGCCCTACGAAACCGATTACCGCGTGCGCGTGCGCATCGACGGCCTGCTCAAGACCGTGGCCCGCGCGCCGGTCAAG
CTGAGCCAGCGCATCGCCGCGCGCCTGAAGGTGATGGCGCAGCTGGACATCGCCGAGAAGCGCGTGCCGCAGGACGGCCG
CATCAAGCTCAACCTGTCCAAGACCAAGCAGATCGACTTCCGCGTCAGCACCCTGCCGACCCTGTTCGGCGAGAAGGTGG
TGCTGCGTATCCTGGACGGCAGCGCGGCCAAGCTGGGCATCGACAAGCTGGGTTACGAGCCCGACCAGCAGAAGCTGTTC
ATGGATGCGATCCAGAAGCCCTACGGCATGGTCCTGGTGACCGGCCCCACCGGCTCGGGCAAGACCGTGTCGCTGTACAC
CGCGCTGGGCATCCTCAACGACGACCTGCGCAACATCTCCACGGTCGAGGACCCGGTGGAAATCCGCCTGCCGGGCGTCA
ACCAGGTGCAGCAGAACGTCAAGCGCGGCATGACCTTCGCCGCTGCGCTGCGCAGCTTCCTGCGCCAGGATCCGGACATC
ATCATGGTCGGCGAAATCCGCGACCTGGAAACGGCCGAAATCGCGATCAAGGCTGCGCAGACCGGCCACATGGTGTTGTC
GACCCTGCACACCAACGACGGCCCGCAGACCATCGCGCGCCTGATGAACATGGGCATCGCCCCGTACAACATCACCTCGT
CGGTGACCCTGGTCATCGCCCAGCGCCTGGCGCGACGGCTGTGCAGCAAGTGCAAGAAGGCGGTGGTCCTGCCCGAGCAT
GCCCTGCTGGCGGAGGGTTTCACCGAAGACGAGATCAAGACCGGGTTCACCGTGTACGAGGCGGTTGGCTGCGAGGACTG
CACCGAGGGCTACAAGGGCCGCACCGGCATCTACCAGGTGATGCCGATGAGCGAGGAGATCCAGGCCATCGTGCTGGAAG
GCGGCAACGCCATGCAGATCGCCGAAGTGGCCACGCGCGCCGGCATCAACGACCTGCGCCGCTCGGCCCTGCTCAAGGTG
CGGCAGGGCATCATCAGCCTGGCCGAGATCAACCGCGTCACCAAGGACTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(51-151)

Type II/IV secretion system protein (T2SSE)

(199-466)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

55.556

98.438

0.547
  pilB Acinetobacter baumannii D1279779

54.674

98.438

0.538
  pilB Legionella pneumophila strain ERS1305867

51.232

98.611

0.505
  pilF Neisseria gonorrhoeae MS11

48.763

98.264

0.479
  pilB Vibrio cholerae strain A1552

48.736

96.181

0.469
  pilB Vibrio parahaemolyticus RIMD 2210633

45.081

97.049

0.438
  pilB Vibrio campbellii strain DS40M4

43.993

98.264

0.432
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

35.966

100

0.372

Multiple sequence alignment