Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   ACEUKD_RS13545 Genome accession   NZ_CP169050
Coordinates   2900475..2901491 (-) Length   338 a.a.
NCBI ID   WP_374092744.1    Uniprot ID   -
Organism   Vibrio diabolicus strain SF42     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2895475..2906491
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACEUKD_RS13520 (ACEUKD_13520) aroK 2896351..2896869 (-) 519 WP_005381319.1 shikimate kinase AroK -
  ACEUKD_RS13525 (ACEUKD_13525) pilQ 2897057..2898793 (-) 1737 WP_374092745.1 type IV pilus secretin PilQ Machinery gene
  ACEUKD_RS13530 (ACEUKD_13530) pilP 2898825..2899340 (-) 516 WP_005395715.1 pilus assembly protein PilP Machinery gene
  ACEUKD_RS13535 (ACEUKD_13535) pilO 2899330..2899923 (-) 594 WP_012841351.1 type 4a pilus biogenesis protein PilO Machinery gene
  ACEUKD_RS13540 (ACEUKD_13540) pilN 2899916..2900491 (-) 576 WP_005395717.1 PilN domain-containing protein Machinery gene
  ACEUKD_RS13545 (ACEUKD_13545) pilM 2900475..2901491 (-) 1017 WP_374092744.1 type IV pilus biogenesis protein PilM Machinery gene
  ACEUKD_RS13550 (ACEUKD_13550) - 2901635..2904196 (+) 2562 WP_074191325.1 penicillin-binding protein 1A -
  ACEUKD_RS13555 (ACEUKD_13555) oxyR 2904248..2905159 (-) 912 WP_005395720.1 DNA-binding transcriptional regulator OxyR -
  ACEUKD_RS13560 (ACEUKD_13560) - 2905330..2906058 (+) 729 WP_005395721.1 redoxin family protein -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 37647.97 Da        Isoelectric Point: 6.8317

>NTDB_id=1049566 ACEUKD_RS13545 WP_374092744.1 2900475..2901491(-) (pilM) [Vibrio diabolicus strain SF42]
MDKLIVTGIDIGHNSLKAVVLKPTGNTYALLGYKEILLKEGIVAENNTINHQEIVKTLKLIKKELPFGAKKVAISVPDNS
VISKKLQIEQNLEDSEVEFAVTQAFSHQSPFPVEELSLDFVRLRAEDGLRGTDNYQVFATRKDVVDSRVDALQKSGLKPV
LVDVHSQSLGHIWKLTADRFPDKNKYCLLDIGTSSSSFTMFTEHGELFHKEFACGTRGANSPVQDDLMSDDAQEKTELFN
RQIVERVKRQMQLYTSINGSQNIKGIWLSGEGASTPMLAEELSQQLSLECELLNPLGLFEMTVSKRKRRAADWQHFSTAA
GLAIRGIHWLGGARALSH

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=1049566 ACEUKD_RS13545 WP_374092744.1 2900475..2901491(-) (pilM) [Vibrio diabolicus strain SF42]
ATGGATAAGCTAATCGTTACCGGCATCGATATAGGCCATAACAGCCTCAAAGCCGTAGTACTTAAACCCACAGGCAACAC
TTACGCTCTGTTGGGTTACAAAGAAATATTACTAAAGGAAGGTATTGTCGCTGAAAATAACACTATAAATCATCAAGAAA
TTGTAAAGACCCTAAAGCTGATTAAAAAAGAACTGCCTTTTGGTGCTAAAAAGGTCGCAATTTCAGTGCCTGATAATTCG
GTGATCAGTAAAAAATTGCAAATAGAACAAAATCTTGAAGATAGCGAAGTTGAGTTTGCTGTGACTCAGGCATTTTCTCA
TCAATCGCCATTCCCTGTCGAAGAGCTGAGTTTAGACTTCGTCCGTCTTCGTGCCGAAGATGGGTTGCGTGGGACTGATA
ACTATCAAGTGTTTGCGACAAGAAAAGATGTGGTCGATAGTCGCGTGGATGCATTACAAAAATCCGGACTAAAACCCGTT
TTAGTGGACGTGCATTCACAAAGTCTTGGTCATATCTGGAAGCTCACCGCCGATCGCTTTCCTGACAAAAATAAGTACTG
CTTACTCGATATTGGCACCTCATCGAGCTCCTTTACCATGTTTACCGAACACGGTGAGCTGTTTCACAAAGAGTTTGCTT
GCGGCACTCGAGGAGCAAACAGCCCTGTGCAAGATGATTTGATGTCCGATGATGCTCAGGAGAAAACAGAGCTGTTTAAT
CGCCAAATCGTGGAACGGGTGAAACGTCAGATGCAGCTCTACACCTCAATTAATGGCTCGCAAAATATCAAAGGTATCTG
GTTATCAGGGGAAGGAGCTTCGACACCAATGCTCGCGGAAGAGTTATCTCAGCAACTGTCACTAGAGTGTGAATTGCTAA
ACCCGCTAGGGCTATTTGAGATGACGGTGTCCAAACGTAAGCGTCGTGCCGCGGATTGGCAGCATTTCTCTACAGCCGCA
GGACTCGCGATTCGTGGCATTCATTGGCTAGGAGGTGCGCGTGCTTTATCGCATTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(8-326)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio campbellii strain DS40M4

81.953

100

0.82
  pilM Vibrio cholerae strain A1552

51.045

99.112

0.506

Multiple sequence alignment