Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGB   Type   Machinery gene
Locus tag   OWO33_RS01130 Genome accession   NZ_OX461090
Coordinates   199271..200314 (+) Length   347 a.a.
NCBI ID   WP_223295889.1    Uniprot ID   -
Organism   Streptococcus agalactiae isolate MRI Z2-336     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 194271..205314
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OWO33_RS01120 - 197877..198242 (+) 366 WP_000285373.1 DUF1033 family protein -
  OWO33_RS01125 comGA 198406..199377 (+) 972 WP_000250805.1 competence type IV pilus ATPase ComGA Machinery gene
  OWO33_RS01130 comGB 199271..200314 (+) 1044 WP_223295889.1 competence type IV pilus assembly protein ComGB Machinery gene
  OWO33_RS01135 - 200391..201836 (-) 1446 WP_000633501.1 recombinase family protein -
  OWO33_RS01140 - 201960..202487 (-) 528 WP_000734161.1 hypothetical protein -
  OWO33_RS01145 - 202504..203247 (-) 744 WP_001260003.1 S24 family peptidase -
  OWO33_RS01150 - 203429..203668 (+) 240 WP_000217789.1 helix-turn-helix transcriptional regulator -
  OWO33_RS01155 - 203681..204409 (+) 729 WP_001004151.1 phage antirepressor protein -
  OWO33_RS01160 - 204474..204653 (+) 180 WP_000806920.1 hypothetical protein -
  OWO33_RS01165 - 204657..205022 (+) 366 WP_001163841.1 hypothetical protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 39936.65 Da        Isoelectric Point: 9.9002

>NTDB_id=1044486 OWO33_RS01130 WP_223295889.1 199271..200314(+) (comGB) [Streptococcus agalactiae isolate MRI Z2-336]
MDKWISWLKKDISVRNRHKSKKLSLKKQRKVVQLFNNLFASGFSLTDMVTFLKRSKLLSDCYTDSMNKALLEGKDLSKML
GELGFSDTVITQVALADLHGNISRSLLKIESYLANLLLVRKKVIEVATYPLILLSFLVLIMIGLRNYLMPQLGENNFATR
LITNVPNIFLLLLAVVLIFSLIFYIIQKRLSRIKVACFLTTIPLVGSYVKLYLTAYYAREWGNLLSQGIELDQIVKVMQN
QKSKLFREIGYDMEEGFLSGKAFHQKVLDYPFFLTELSLMIEYGQVKAKLGTELDIYADEKWEDFFTKLARATQLIQPVI
FIFVALIIVMIYAAMLLPMYQNMEILS

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=1044486 OWO33_RS01130 WP_223295889.1 199271..200314(+) (comGB) [Streptococcus agalactiae isolate MRI Z2-336]
ATAGACAAGTGGATATCTTGGCTGAAGAAGGACATATCAGTAAGAAACAGGCACAAGTCGAAAAAATTATCCCTCAAGAA
ACAACGGAAAGTAGTCCAACTTTTTAATAATCTTTTTGCTAGTGGTTTTTCTTTAACTGATATGGTCACCTTCTTAAAGA
GGAGTAAGTTATTGTCTGATTGTTATACAGATAGTATGAATAAGGCATTATTAGAGGGAAAAGATTTATCAAAAATGTTA
GGAGAGTTAGGTTTTTCTGACACTGTTATCACACAGGTTGCATTAGCTGATTTGCATGGTAACATTTCAAGGAGCCTACT
AAAGATTGAGTCTTATTTAGCTAATCTTTTGTTAGTTAGAAAAAAAGTAATAGAAGTAGCTACTTACCCATTGATATTAT
TGTCTTTTCTGGTGCTAATTATGATTGGCCTTAGGAATTATTTAATGCCCCAATTAGGAGAAAATAATTTTGCAACTAGA
CTGATTACAAATGTGCCGAATATTTTCTTATTACTTTTAGCAGTTGTACTTATTTTTAGTTTAATATTTTATATTATTCA
AAAGCGATTGTCGCGCATTAAAGTAGCTTGTTTTTTAACAACAATTCCCTTAGTTGGATCATATGTTAAGCTTTATTTAA
CTGCTTACTATGCCCGTGAGTGGGGAAATTTATTAAGTCAAGGTATTGAATTGGACCAAATTGTTAAAGTAATGCAAAAT
CAGAAATCCAAACTTTTTAGGGAAATAGGATATGACATGGAAGAAGGTTTTCTATCAGGTAAAGCATTTCACCAAAAAGT
ATTAGACTATCCGTTTTTCTTAACTGAGCTTAGTTTAATGATTGAATATGGCCAAGTTAAGGCGAAATTAGGAACAGAGT
TAGATATATATGCTGATGAGAAGTGGGAGGATTTTTTTACAAAATTAGCTAGAGCGACCCAGTTAATCCAACCCGTTATT
TTTATTTTTGTAGCTCTTATCATTGTTATGATTTATGCAGCAATGCTGTTACCAATGTATCAAAATATGGAGATATTATC
ATGA

Domains

Predicted by InterProScan.

Type II secretion system (T2SS), protein F (T2SSF)

(34-150)

Type II secretion system (T2SS), protein F (T2SSF)

(218-338)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB Streptococcus mutans UA140

62.17

98.271

0.611
  comGB Streptococcus mutans UA159

61.877

98.271

0.608
  comGB Streptococcus gordonii str. Challis substr. CH1

56.305

98.271

0.553
  comGB/cglB Streptococcus mitis NCTC 12261

55.224

96.542

0.533
  comGB/cglB Streptococcus mitis SK321

54.627

96.542

0.527
  comGB/cglB Streptococcus pneumoniae Rx1

53.731

96.542

0.519
  comGB/cglB Streptococcus pneumoniae D39

53.731

96.542

0.519
  comGB/cglB Streptococcus pneumoniae R6

53.731

96.542

0.519
  comGB/cglB Streptococcus pneumoniae TIGR4

53.731

96.542

0.519
  comGB Lactococcus lactis subsp. cremoris KW2

50.148

97.118

0.487