Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   ACEN8S_RS12425 Genome accession   NZ_CP168517
Coordinates   2793110..2793979 (+) Length   289 a.a.
NCBI ID   WP_413623904.1    Uniprot ID   -
Organism   Luteibacter sp. Lutesp34 strain CT11-34     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2788110..2798979
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACEN8S_RS12410 (ACEN8S_12410) - 2788872..2789726 (-) 855 WP_413623901.1 glycosyltransferase family 2 protein -
  ACEN8S_RS12415 (ACEN8S_12415) pilB 2789894..2791621 (+) 1728 WP_413623902.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACEN8S_RS12420 (ACEN8S_12420) pilC 2791766..2793031 (+) 1266 WP_413623903.1 type II secretion system F family protein Machinery gene
  ACEN8S_RS12425 (ACEN8S_12425) pilD 2793110..2793979 (+) 870 WP_413623904.1 prepilin peptidase Machinery gene
  ACEN8S_RS12430 (ACEN8S_12430) coaE 2793976..2794578 (+) 603 WP_413623905.1 dephospho-CoA kinase -
  ACEN8S_RS12435 (ACEN8S_12435) - 2794692..2795942 (-) 1251 WP_413623906.1 putative bifunctional diguanylate cyclase/phosphodiesterase -
  ACEN8S_RS12440 (ACEN8S_12440) - 2795939..2797186 (-) 1248 WP_413623907.1 HD-GYP domain-containing protein -
  ACEN8S_RS12445 (ACEN8S_12445) msrA 2797349..2798023 (+) 675 WP_413623908.1 peptide-methionine (S)-S-oxide reductase MsrA -

Sequence

Protein


Download         Length: 289 a.a.        Molecular weight: 31730.70 Da        Isoelectric Point: 8.4600

>NTDB_id=1044444 ACEN8S_RS12425 WP_413623904.1 2793110..2793979(+) (pilD) [Luteibacter sp. Lutesp34 strain CT11-34]
MPELPLALWIALAAVFGMVVGSFLNVVILRTPARMQWEWRRQAREVLELEAVDDPKPPGVALEASHCPKCKHRLAAYDNI
PVLGWLFLRGRCRYCGTKISAQYPLVELFTGVASALVVWHFGPTPAALAGLVFTFFLVALSGIDARTQFLPDELNYPLLW
IGLGLSLIPAWQPLPVEPSSAILAALIGYLSLWSVYWLFKLLTGKEGMGYGDFKLLAALGAWMGPVSLLPVIMLSSLIGA
LVGGGLMLFRNHQRDVPIPFGPYIAAAGWVWFLAGDRLLAAYLQFTGLR

Nucleotide


Download         Length: 870 bp        

>NTDB_id=1044444 ACEN8S_RS12425 WP_413623904.1 2793110..2793979(+) (pilD) [Luteibacter sp. Lutesp34 strain CT11-34]
ATGCCCGAACTCCCGCTCGCTCTCTGGATCGCCCTGGCCGCCGTTTTCGGCATGGTCGTCGGCAGCTTCCTCAACGTCGT
CATCCTGCGCACGCCTGCGCGCATGCAGTGGGAATGGCGCCGGCAGGCGCGCGAGGTGCTGGAACTGGAAGCGGTCGACG
ACCCGAAACCGCCGGGCGTGGCACTGGAAGCCTCGCACTGCCCGAAGTGCAAACACCGGCTGGCCGCCTACGACAACATT
CCCGTATTGGGTTGGCTGTTCCTGCGCGGGCGCTGCCGTTACTGCGGCACGAAGATCTCGGCCCAGTATCCGCTGGTGGA
ACTGTTCACCGGCGTGGCCAGCGCTCTGGTGGTCTGGCACTTCGGCCCCACGCCGGCGGCGCTGGCGGGCCTGGTTTTCA
CGTTTTTCCTCGTCGCCCTCTCCGGGATCGACGCCCGGACGCAGTTCCTGCCGGACGAACTCAATTACCCCCTGCTCTGG
ATCGGCCTGGGCCTGTCGCTGATTCCCGCCTGGCAGCCGCTGCCGGTGGAGCCCTCTTCCGCCATCCTGGCGGCGCTGAT
CGGTTATCTCAGCCTGTGGAGCGTCTACTGGCTGTTCAAGCTGCTGACGGGCAAGGAAGGCATGGGCTACGGCGACTTCA
AACTGCTGGCCGCCCTCGGCGCGTGGATGGGGCCCGTGTCCCTCTTGCCGGTCATCATGCTGTCGTCCCTCATCGGCGCC
CTGGTGGGCGGCGGTCTCATGCTCTTCCGAAATCACCAGCGCGACGTGCCGATTCCATTCGGCCCCTATATCGCCGCCGC
GGGTTGGGTGTGGTTCCTGGCCGGCGATCGCCTGCTGGCGGCCTACCTGCAATTCACCGGGCTGCGATGA

Domains

Predicted by InterproScan.

Type IV leader peptidase family (Peptidase_A24)

(131-243)

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(15-121)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Acinetobacter baumannii D1279779

50

96.194

0.481
  pilD Acinetobacter nosocomialis M2

49.64

96.194

0.478
  pilD Vibrio cholerae strain A1552

47.464

95.502

0.453
  pilD Vibrio campbellii strain DS40M4

46.57

95.848

0.446
  pilD Neisseria gonorrhoeae MS11

47.601

93.772

0.446

Multiple sequence alignment