Detailed information NTG1044442

Bioinformatically predicted

Overview

• Name: pilB
• Type: Machinery gene
• Locus tag: ACEN8S_RS12415
• Genome accession: NZ_CP168517
• Coordinates: 2789894..2791621 (+)
• Length: 575 a.a.
• NCBI ID: WP_413623902.1
• Uniprot ID: -
• Organism: Luteibacter sp. Lutesp34 strain CT11-34
• Function: power the assembly of type IV pilus (predicted from homology) DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type	MGE coordinates	Gene coordinates	Relative position	Distance (bp)
Genomic island	2775954..2789726	2789894..2791621	flank	168

Gene organization within MGE regions

Location: 2775954..2791621

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACEN8S_RS12355 (ACEN8S_12355)	pilE	2776310..2776753 (+)	444	WP_413623890.1	pilin	Machinery gene
ACEN8S_RS12360 (ACEN8S_12360)	-	2776832..2778871 (+)	2040	WP_413623891.1	tetratricopeptide repeat protein	-
ACEN8S_RS12365 (ACEN8S_12365)	-	2778868..2780826 (+)	1959	WP_413623892.1	hypothetical protein	-
ACEN8S_RS12370 (ACEN8S_12370)	-	2780823..2781929 (+)	1107	WP_413623893.1	glycosyltransferase family 4 protein	-
ACEN8S_RS12375 (ACEN8S_12375)	-	2781963..2783138 (+)	1176	WP_413623894.1	aminotransferase class I/II-fold pyridoxal phosphate-dependent enzyme	-
ACEN8S_RS12380 (ACEN8S_12380)	-	2783135..2783773 (+)	639	WP_413623895.1	NeuD/PglB/VioB family sugar acetyltransferase	-
ACEN8S_RS12385 (ACEN8S_12385)	-	2783781..2785208 (+)	1428	WP_413626756.1	lipopolysaccharide biosynthesis protein	-
ACEN8S_RS12390 (ACEN8S_12390)	-	2785213..2786145 (+)	933	WP_413623896.1	NAD-dependent epimerase/dehydratase family protein	-
ACEN8S_RS12395 (ACEN8S_12395)	-	2786142..2787143 (+)	1002	WP_413623897.1	glycosyltransferase family 2 protein	-
ACEN8S_RS12400 (ACEN8S_12400)	-	2787140..2787919 (-)	780	WP_413623898.1	class I SAM-dependent methyltransferase	-
ACEN8S_RS12405 (ACEN8S_12405)	-	2787922..2788875 (-)	954	WP_413623900.1	glycosyltransferase	-
ACEN8S_RS12410 (ACEN8S_12410)	-	2788872..2789726 (-)	855	WP_413623901.1	glycosyltransferase family 2 protein	-
ACEN8S_RS12415 (ACEN8S_12415)	pilB	2789894..2791621 (+)	1728	WP_413623902.1	type IV-A pilus assembly ATPase PilB	Machinery gene

Sequence

Protein

• Length: 575 a.a.
• Molecular weight: 62575.27 Da
• Isoelectric Point: 6.5167

>NTDB_id=1044442 ACEN8S_RS12415 WP_413623902.1 2789894..2791621(+) (pilB) [Luteibacter sp. Lutesp34 strain CT11-34]
MASQMHTPILAGLTGMARRLVTESVLPEADVRKAVQDAAEKRVSLSAWLVDHNLVDSAKLSQVASAEFGMPLMDISGMAT
ANMPLDLVTEALVTKHQALPLFKRGKRLFVGIADPMQSHALDEIKFHSNHMVEPVLVERGQLRRIIDTALSAMSATVPGF
EDNALEDLALESGGEDGESATGIDANANDDAPVVKFVNKILVDAIKRGASDIHFEPFETVYRVRLRMDGILRVVATAPIK
LGNRIASRIKVMSGLDIAERRVPQDGRIKLNLTKTRAIDFRVSTLPTLFGEKIVLRILDGSSAKLGIDKLGYEDAQKKLY
LDAIEKPYGMVLVTGPTGSGKTVSLYTALNILNTEGRNISTVEDPVEIRVEGINQVQQNTKRGMTFAAALRSFLRQDPDV
IMVGEIRDLETAEIAVKAAQTGHMVLSTLHTNDAPQTISRLMNMGIAPYNITSSVTLIIAQRLARRLHDCKRAIQLPPAA
LLAEGFTQEEIDEGITIYEAVGCDSCNEGYKGRVGIYQVMPMVEDIQKIVLEGGNAMQIAEVAKKAGINDLRASALLKVR
NGVTSLAEINRVTKD

Nucleotide

• Length: 1728 bp

>NTDB_id=1044442 ACEN8S_RS12415 WP_413623902.1 2789894..2791621(+) (pilB) [Luteibacter sp. Lutesp34 strain CT11-34]
ATGGCTTCACAAATGCATACGCCGATCCTGGCTGGCCTTACCGGCATGGCGCGACGGCTGGTGACGGAGAGCGTCCTGCC
GGAGGCCGATGTCCGCAAGGCCGTGCAGGATGCGGCGGAGAAGCGCGTCTCCCTTTCCGCCTGGCTGGTCGACCACAACC
TGGTCGATAGTGCCAAGCTGTCCCAAGTCGCTTCGGCCGAGTTCGGCATGCCCCTGATGGACATCAGCGGCATGGCCACC
GCCAACATGCCTCTGGACCTGGTGACCGAAGCACTTGTCACGAAGCACCAGGCGCTGCCCCTTTTCAAGCGCGGCAAGCG
GCTCTTCGTCGGCATTGCCGATCCCATGCAGTCGCATGCGCTGGACGAGATCAAGTTCCACTCGAACCACATGGTCGAAC
CGGTGTTGGTCGAGCGCGGCCAGCTGCGCCGGATCATCGACACGGCACTTTCGGCCATGAGCGCCACGGTGCCCGGCTTC
GAGGACAATGCGCTCGAAGACCTGGCGCTGGAAAGCGGCGGTGAAGACGGCGAAAGCGCCACGGGCATCGACGCCAACGC
CAACGACGACGCCCCGGTCGTCAAGTTCGTCAACAAGATCCTGGTGGACGCCATCAAGCGTGGCGCCTCCGATATCCATT
TCGAGCCGTTCGAGACCGTCTACCGCGTCCGCCTGCGCATGGACGGCATCCTGCGCGTCGTCGCCACGGCCCCGATCAAG
CTGGGCAATCGCATCGCCTCGCGCATCAAGGTCATGAGCGGCCTGGACATCGCGGAACGCCGCGTGCCACAGGACGGCCG
CATCAAGCTCAACCTGACCAAGACCCGCGCCATCGACTTCCGCGTAAGCACCCTGCCGACGCTGTTCGGCGAGAAGATCG
TGCTGCGTATCCTCGACGGCTCCTCGGCCAAGCTGGGCATCGACAAGCTGGGTTACGAGGACGCGCAGAAGAAGCTCTAC
CTCGACGCCATCGAAAAACCTTACGGCATGGTGCTGGTCACCGGTCCCACCGGCTCGGGCAAGACGGTGTCGCTCTACAC
CGCCCTCAATATCCTCAACACCGAGGGGCGCAACATCTCCACGGTGGAAGACCCGGTCGAAATCCGCGTGGAGGGTATCA
ACCAGGTCCAGCAGAACACCAAGCGCGGCATGACCTTCGCGGCCGCACTGCGTTCGTTCCTGCGCCAGGATCCGGACGTC
ATCATGGTCGGCGAAATCCGCGACCTCGAAACGGCCGAGATTGCAGTGAAAGCGGCGCAGACCGGCCACATGGTGCTTTC
CACCCTGCACACTAACGACGCGCCGCAGACCATCTCGCGCCTGATGAACATGGGCATCGCGCCCTACAACATTACCTCGT
CGGTCACCCTGATCATCGCCCAGCGCCTGGCGCGGCGCCTGCACGACTGCAAGCGCGCGATCCAACTGCCGCCGGCGGCC
CTGCTGGCCGAGGGTTTTACCCAGGAAGAAATCGACGAGGGCATCACCATCTACGAGGCCGTCGGCTGCGACAGCTGCAA
CGAGGGCTATAAGGGCCGCGTCGGCATCTACCAGGTCATGCCCATGGTCGAGGACATCCAGAAGATCGTCCTGGAGGGCG
GCAACGCTATGCAGATCGCCGAAGTGGCGAAGAAGGCCGGCATCAACGACCTCCGTGCCTCGGCGTTGCTGAAGGTCCGA
AACGGCGTCACCAGCCTGGCGGAGATCAACCGCGTCACCAAGGACTGA

Domains

Predicted by InterproScan.

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (49-152)

• Type II/IV secretion system protein (T2SSE) (199-466)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilB	Acinetobacter baumannii D1279779	54.152	100	0.544
pilB	Acinetobacter baylyi ADP1	53.805	98.261	0.529
pilB	Legionella pneumophila strain ERS1305867	50.176	98.783	0.496
pilB	Vibrio cholerae strain A1552	48.754	97.739	0.477
pilB	Vibrio campbellii strain DS40M4	46.996	98.435	0.463
pilF	Neisseria gonorrhoeae MS11	49.906	92.348	0.461
pilB	Vibrio parahaemolyticus RIMD 2210633	48.327	93.565	0.452
pilB	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	38.095	94.957	0.362