Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ACEN8S_RS04175 Genome accession   NZ_CP168517
Coordinates   919468..920631 (+) Length   387 a.a.
NCBI ID   WP_167025371.1    Uniprot ID   -
Organism   Luteibacter sp. Lutesp34 strain CT11-34     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 914468..925631
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACEN8S_RS04155 (ACEN8S_04155) - 914561..916666 (+) 2106 WP_413625736.1 bifunctional (p)ppGpp synthetase/guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase -
  ACEN8S_RS04160 (ACEN8S_04160) - 916828..917403 (-) 576 WP_413625737.1 YggT family protein -
  ACEN8S_RS04165 (ACEN8S_04165) proC 917400..918224 (-) 825 WP_413625738.1 pyrroline-5-carboxylate reductase -
  ACEN8S_RS04170 (ACEN8S_04170) pilT 918347..919384 (+) 1038 WP_167129031.1 type IV pilus twitching motility protein PilT Machinery gene
  ACEN8S_RS04175 (ACEN8S_04175) pilU 919468..920631 (+) 1164 WP_167025371.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACEN8S_RS04180 (ACEN8S_04180) - 920683..921048 (-) 366 WP_413625739.1 BON domain-containing protein -
  ACEN8S_RS04185 (ACEN8S_04185) - 921294..923042 (-) 1749 WP_413625740.1 hypothetical protein -
  ACEN8S_RS04190 (ACEN8S_04190) - 923166..924092 (-) 927 WP_413625741.1 aspartate carbamoyltransferase catalytic subunit -
  ACEN8S_RS04195 (ACEN8S_04195) ruvX 924171..924590 (-) 420 WP_413625742.1 Holliday junction resolvase RuvX -
  ACEN8S_RS04200 (ACEN8S_04200) - 924604..925170 (-) 567 WP_413625743.1 YqgE/AlgH family protein -
  ACEN8S_RS04205 (ACEN8S_04205) - 925262..925582 (+) 321 WP_413625744.1 hypothetical protein -

Sequence

Protein


Download         Length: 387 a.a.        Molecular weight: 43369.61 Da        Isoelectric Point: 6.8595

>NTDB_id=1044434 ACEN8S_RS04175 WP_167025371.1 919468..920631(+) (pilU) [Luteibacter sp. Lutesp34 strain CT11-34]
MSDFDFTSFLKLMVHKQASDLFITAGVAPSMKVQGRIVPITQSPLSAQQSRDMVLNVMTPAQREEFEKTHECQFAISASG
IGRFRVSCFYQRNCVGMVLRRIESKIPTPEELSLPPIIKQLAMTKRGIIIFVGGTGTGKSTSLASMIGYRNQNSTGHIIT
IEDPIEYVHKHEGCIITQRELGIDTDSWENALKNTLRQAPDVIMIGEVRTRETMEHAINFSETGHLCLCTLHANNANQAL
DRILHFFPEDRRQQLFMDLSLNLKGIVAQQLIPTPDGKARRVAVEVLLGTPLAQDYIRQGEIHKLKELMRDSNQLGMRTF
DQSLVELYHAGEISYEDALRHADSSNEVRLRIKLAQGGDAHTLSQGLEGVEIDKSNDRDSFGGMLHR

Nucleotide


Download         Length: 1164 bp        

>NTDB_id=1044434 ACEN8S_RS04175 WP_167025371.1 919468..920631(+) (pilU) [Luteibacter sp. Lutesp34 strain CT11-34]
ATGAGCGACTTCGATTTCACCTCCTTCCTCAAGCTGATGGTGCACAAGCAGGCCTCCGACCTGTTCATCACCGCCGGCGT
CGCGCCGTCCATGAAGGTGCAGGGGCGCATCGTGCCCATCACGCAGAGCCCGTTGTCGGCGCAGCAGTCGCGCGACATGG
TGCTGAACGTGATGACGCCCGCCCAGCGCGAGGAATTCGAGAAAACCCACGAGTGCCAGTTCGCGATCAGCGCCTCGGGC
ATCGGGCGGTTCCGCGTGTCCTGCTTCTACCAGCGCAACTGCGTGGGCATGGTGCTGCGCCGGATCGAGTCGAAGATTCC
CACACCGGAAGAATTGAGCCTGCCGCCGATCATCAAGCAACTGGCGATGACCAAGCGCGGCATCATCATCTTCGTCGGCG
GTACGGGTACCGGTAAGTCGACCTCGCTGGCGTCGATGATCGGCTACCGCAACCAGAACTCCACGGGCCACATCATCACC
ATCGAGGACCCGATCGAGTACGTGCACAAGCACGAAGGTTGCATCATCACCCAGCGCGAACTCGGCATCGATACCGACAG
CTGGGAAAACGCGCTCAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAAGTGCGCACGCGCGAGACGA
TGGAACATGCCATCAACTTCTCGGAGACGGGCCACCTCTGTCTGTGCACGCTGCATGCGAACAACGCCAACCAGGCGCTG
GACCGCATCCTGCACTTCTTCCCCGAAGACCGCCGCCAGCAGCTCTTCATGGACCTCTCCCTCAACCTGAAGGGCATCGT
GGCGCAGCAGCTCATTCCCACCCCGGACGGCAAGGCCCGTCGCGTGGCGGTGGAAGTTCTGCTGGGCACGCCGCTTGCGC
AGGACTACATCCGCCAGGGCGAGATCCACAAGCTGAAGGAACTGATGCGGGACTCGAACCAGCTCGGCATGCGCACGTTC
GACCAGAGCCTGGTGGAGCTCTACCACGCGGGCGAAATTTCCTATGAGGACGCGCTGCGCCACGCGGACAGTTCCAACGA
GGTCCGGCTGCGCATCAAGCTGGCCCAGGGCGGCGACGCGCACACCCTGTCGCAGGGGCTGGAAGGCGTGGAGATCGACA
AGAGCAACGACCGCGACTCGTTTGGCGGCATGCTGCATCGCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(117-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

66.578

96.641

0.643
  pilU Acinetobacter baylyi ADP1

64.045

91.99

0.589
  pilU Vibrio cholerae strain A1552

51.622

95.607

0.494
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.247

96.124

0.377

Multiple sequence alignment