Detailed information NTG1040805

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: ACDW37_RS01975
• Genome accession: NZ_CP167985
• Coordinates: 420775..421923 (+)
• Length: 382 a.a.
• NCBI ID: WP_003100959.1
• Uniprot ID: G3XCX3
• Organism: Pseudomonas aeruginosa strain 0502d1
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 415775..426923

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACDW37_RS01950 (ACDW37_01950)	-	416926..417222 (-)	297	WP_031628111.1	DUF167 domain-containing protein	-
ACDW37_RS01955 (ACDW37_01955)	-	417219..417812 (-)	594	WP_003084544.1	YggT family protein	-
ACDW37_RS01960 (ACDW37_01960)	proC	417823..418644 (-)	822	WP_003084546.1	pyrroline-5-carboxylate reductase	-
ACDW37_RS01965 (ACDW37_01965)	-	418656..419348 (-)	693	WP_003084549.1	YggS family pyridoxal phosphate-dependent enzyme	-
ACDW37_RS01970 (ACDW37_01970)	pilT	419563..420597 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
ACDW37_RS01975 (ACDW37_01975)	pilU	420775..421923 (+)	1149	WP_003100959.1	type IV pilus ATPase PilU	Machinery gene
ACDW37_RS01980 (ACDW37_01980)	czcD	421931..422830 (-)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
ACDW37_RS01985 (ACDW37_01985)	-	422984..423388 (+)	405	WP_003084560.1	NINE protein	-
ACDW37_RS01990 (ACDW37_01990)	-	423631..425004 (+)	1374	WP_003100955.1	pyridoxal-phosphate dependent enzyme	-
ACDW37_RS01995 (ACDW37_01995)	-	425001..426185 (+)	1185	WP_015649763.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42532.81 Da
• Isoelectric Point: 6.7792

>NTDB_id=1040805 ACDW37_RS01975 WP_003100959.1 420775..421923(+) (pilU) [Pseudomonas aeruginosa strain 0502d1]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSSLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=1040805 ACDW37_RS01975 WP_003100959.1 420775..421923(+) (pilU) [Pseudomonas aeruginosa strain 0502d1]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAGCCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: G3XCX3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.482	100	0.885
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382