Detailed information NTG1040771

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: ACDW36_RS02295
• Genome accession: NZ_CP167984
• Coordinates: 513684..514832 (+)
• Length: 382 a.a.
• NCBI ID: WP_003110016.1
• Uniprot ID: A0A0H2ZL88
• Organism: Pseudomonas aeruginosa strain 0304d2
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 508684..519832

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACDW36_RS02270 (ACDW36_02270)	-	509835..510131 (-)	297	WP_031628111.1	DUF167 domain-containing protein	-
ACDW36_RS02275 (ACDW36_02275)	-	510128..510721 (-)	594	WP_003084544.1	YggT family protein	-
ACDW36_RS02280 (ACDW36_02280)	proC	510732..511553 (-)	822	WP_003084546.1	pyrroline-5-carboxylate reductase	-
ACDW36_RS02285 (ACDW36_02285)	-	511565..512257 (-)	693	WP_003084549.1	YggS family pyridoxal phosphate-dependent enzyme	-
ACDW36_RS02290 (ACDW36_02290)	pilT	512472..513506 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
ACDW36_RS02295 (ACDW36_02295)	pilU	513684..514832 (+)	1149	WP_003110016.1	type IV pilus ATPase PilU	Machinery gene
ACDW36_RS02300 (ACDW36_02300)	czcD	514840..515739 (-)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
ACDW36_RS02305 (ACDW36_02305)	-	515894..516298 (+)	405	WP_003084560.1	NINE protein	-
ACDW36_RS02310 (ACDW36_02310)	-	516541..517914 (+)	1374	WP_003100955.1	pyridoxal-phosphate dependent enzyme	-
ACDW36_RS02315 (ACDW36_02315)	-	517911..519095 (+)	1185	WP_003110017.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42559.83 Da
• Isoelectric Point: 6.7792

>NTDB_id=1040771 ACDW36_RS02295 WP_003110016.1 513684..514832(+) (pilU) [Pseudomonas aeruginosa strain 0304d2]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSNLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=1040771 ACDW36_RS02295 WP_003110016.1 513684..514832(+) (pilU) [Pseudomonas aeruginosa strain 0304d2]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCATG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGATCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAACCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: A0A0H2ZL88

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.22	100	0.882
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382