Detailed information    

insolico Bioinformatically predicted

Overview

Name   ruvB   Type   Machinery gene
Locus tag   CF58_RS13345 Genome accession   NZ_CP007392
Coordinates   2752388..2753398 (-) Length   336 a.a.
NCBI ID   WP_000568522.1    Uniprot ID   Q8FGR3
Organism   Escherichia coli strain ST2747     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2747388..2758398
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CF58_RS13325 (CF58_17025) mepM 2748352..2749674 (-) 1323 WP_001184045.1 murein DD-endopeptidase MepM -
  CF58_RS13330 (CF58_17030) znuA 2749690..2750622 (-) 933 WP_011076428.1 zinc ABC transporter substrate-binding protein ZnuA -
  CF58_RS13335 (CF58_17035) znuC 2750701..2751456 (+) 756 WP_000202987.1 zinc ABC transporter ATP-binding protein ZnuC -
  CF58_RS13340 (CF58_17040) znuB 2751453..2752238 (+) 786 WP_000571471.1 zinc ABC transporter permease subunit ZnuB -
  CF58_RS13345 (CF58_17050) ruvB 2752388..2753398 (-) 1011 WP_000568522.1 Holliday junction branch migration DNA helicase RuvB Machinery gene
  CF58_RS13350 (CF58_17055) ruvA 2753407..2754018 (-) 612 WP_000580323.1 Holliday junction branch migration protein RuvA -
  CF58_RS26710 yobI 2754157..2754222 (-) 66 WP_072146566.1 stress response small protein YobI -
  CF58_RS13355 (CF58_17070) yebB 2754293..2754895 (+) 603 WP_025210575.1 YebB family permuted papain-like enzyme -
  CF58_RS13360 (CF58_17075) ruvC 2754897..2755418 (-) 522 WP_001295503.1 crossover junction endodeoxyribonuclease RuvC -
  CF58_RS13365 (CF58_17080) yebC 2755453..2756193 (-) 741 WP_000907234.1 YebC/PmpR family DNA-binding transcriptional regulator -
  CF58_RS13370 (CF58_17085) nudB 2756222..2756674 (-) 453 WP_001308712.1 dihydroneopterin triphosphate diphosphatase -

Sequence

Protein


Download         Length: 336 a.a.        Molecular weight: 37171.80 Da        Isoelectric Point: 4.7818

>NTDB_id=104055 CF58_RS13345 WP_000568522.1 2752388..2753398(-) (ruvB) [Escherichia coli strain ST2747]
MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIFIKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMG
VNLRTTSGPVLEKAGDLAAMLTNLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGALEVARRARGTPRIANRLLRRVRDFAEVKHDG
TISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKFFGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMA
TVRAWNHFGITPPEMP

Nucleotide


Download         Length: 1011 bp        

>NTDB_id=104055 CF58_RS13345 WP_000568522.1 2752388..2753398(-) (ruvB) [Escherichia coli strain ST2747]
ATGATTGAAGCAGACCGTCTGATTTCTGCCGGTACCACTTTGCCGGAAGATGTGGCAGATCGCGCCATTCGCCCTAAATT
ACTGGAAGAGTATGTTGGTCAGCCGCAGGTTCGTTCGCAGATGGAGATTTTCATCAAAGCAGCGAAACTGCGCGGCGATG
CCCTCGATCATCTGTTGATTTTTGGTCCTCCGGGGTTGGGTAAAACTACGCTTGCTAACATTGTCGCCAATGAAATGGGT
GTTAATTTACGTACGACTTCTGGTCCGGTGCTGGAAAAGGCGGGCGATCTGGCAGCGATGCTCACTAACCTTGAACCGCA
TGACGTGCTGTTTATTGATGAGATCCACCGTCTTTCACCAGTGGTGGAAGAGGTGTTGTATCCGGCAATGGAAGATTACC
AACTGGATATCATGATTGGTGAAGGTCCGGCGGCACGCTCCATTAAAATTGATTTGCCGCCGTTTACACTGATTGGCGCA
ACCACGCGCGCAGGTTCGCTGACATCACCGTTGCGCGACCGTTTTGGTATTGTGCAACGTCTAGAGTTTTATCAGGTGCC
GGATCTGCAATATATCGTCAGTCGCAGCGCACGCTTTATGGGGCTTGAGATGAGTGATGACGGCGCGCTGGAAGTTGCTC
GTCGCGCTCGCGGTACGCCGCGCATTGCTAACCGTCTGCTGCGTCGAGTGCGTGATTTCGCCGAAGTGAAGCACGATGGC
ACCATCTCGGCTGATATCGCTGCTCAGGCGCTGGATATGCTGAATGTCGATGCCGAAGGTTTCGATTATATGGACCGCAA
ATTGTTGCTGGCGGTAATCGATAAGTTCTTTGGTGGGCCGGTAGGTCTGGATAACCTGGCGGCTGCCATTGGCGAAGAAC
GTGAAACCATTGAGGATGTGCTGGAACCTTATTTGATTCAGCAAGGCTTTTTGCAGCGTACACCGCGCGGGCGTATGGCG
ACAGTGCGGGCGTGGAACCACTTCGGCATCACGCCGCCAGAAATGCCGTAA

Domains

Predicted by InterProScan.

Holliday junction DNA helicase RuvB P-loop domain (RuvB_N)

(23-181)

RuvB AAA lid domain (AAA_lid_4)

(184-257)

RuvB C-terminal winged helix domain (WHD_RuvB)

(259-328)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q8FGR3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ruvB Bacillus subtilis subsp. subtilis str. 168

60.486

97.917

0.592
  ruvB Streptococcus pneumoniae TIGR4

58.934

94.94

0.56
  ruvB Streptococcus pneumoniae R6

58.934

94.94

0.56
  ruvB Streptococcus pneumoniae D39

58.934

94.94

0.56
  ruvB Synechocystis sp. PCC 6803

52.941

96.131

0.509
  ruvB Helicobacter pylori 26695

52.366

94.345

0.494

Multiple sequence alignment