Detailed information NTG1040193

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: ACCM60_RS02230
• Genome accession: NZ_CP167916
• Coordinates: 496989..498041 (+)
• Length: 350 a.a.
• NCBI ID: WP_198919653.1
• Uniprot ID: -
• Organism: Pseudomonas chlororaphis subsp. aureofaciens strain W9-1
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 491989..503041

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACCM60_RS02220 (ACCM60_02220)	-	492941..494209 (+)	1269	WP_198919652.1	malic enzyme-like NAD(P)-binding protein	-
ACCM60_RS02225 (ACCM60_02225)	-	494337..496772 (-)	2436	WP_164485464.1	penicillin-binding protein 1A	-
ACCM60_RS02230 (ACCM60_02230)	pilM	496989..498041 (+)	1053	WP_198919653.1	pilus assembly protein PilM	Machinery gene
ACCM60_RS02235 (ACCM60_02235)	-	498041..498607 (+)	567	WP_009046598.1	PilN domain-containing protein	-
ACCM60_RS02240 (ACCM60_02240)	-	498604..499221 (+)	618	WP_009046599.1	type 4a pilus biogenesis protein PilO	-
ACCM60_RS02245 (ACCM60_02245)	-	499218..499745 (+)	528	WP_103330911.1	pilus assembly protein PilP	-
ACCM60_RS02250 (ACCM60_02250)	pilQ	499759..501831 (+)	2073	WP_009046601.1	type IV pilus secretin PilQ	Machinery gene
ACCM60_RS02255 (ACCM60_02255)	aroK	501836..502354 (+)	519	WP_009041824.1	shikimate kinase AroK	-

Sequence

Protein

• Length: 350 a.a.
• Molecular weight: 37075.56 Da
• Isoelectric Point: 5.1221

>NTDB_id=1040193 ACCM60_RS02230 WP_198919653.1 496989..498041(+) (pilM) [Pseudomonas chlororaphis subsp. aureofaciens strain W9-1]
MLGLFSKKASSLLGIDISSTSVKLLELSRVGGRFRVEAYGVEPLPANAVVEKNIVEPEGVGHALSRLLARARPGAKHAAL
AMAGSAVISKVIEMEAGLCDDDRESQLKVDADQYIPYPLDEAAIDFDVLGYSARNPERVDVLLAACRKEHIEVREAALEL
AGLTARVVDLEVCALERSFALAAGASVARTVALVDIGATLTALSVVHEGQIIYSREQLFGGHRLTEHIQRRYGLSKEEAS
LAKKQGGLPDDYASEVLQPFQEAVVQQVSRSLQFFQASGPQRQVDSILLAGGSASVPGLAPLIEQRLGLPVLIANPFAGM
SIGGKVDAAALASEAPSLMIACGLALRSFD

Nucleotide

• Length: 1053 bp

>NTDB_id=1040193 ACCM60_RS02230 WP_198919653.1 496989..498041(+) (pilM) [Pseudomonas chlororaphis subsp. aureofaciens strain W9-1]
GTGCTAGGACTCTTCAGTAAAAAGGCGAGTTCCCTTCTGGGGATCGATATCAGCTCCACCTCGGTCAAGCTTCTTGAGTT
GAGCCGGGTCGGCGGCCGTTTTCGGGTCGAGGCCTATGGGGTGGAACCGTTGCCGGCCAACGCCGTGGTCGAAAAAAACA
TCGTCGAGCCCGAAGGGGTGGGGCATGCCTTGTCTCGCCTGCTGGCCAGGGCCCGGCCGGGAGCGAAGCACGCAGCGCTG
GCGATGGCCGGCTCGGCGGTGATCAGCAAGGTTATCGAGATGGAGGCCGGGCTTTGCGATGACGATCGGGAGAGCCAGCT
CAAGGTCGACGCCGACCAGTACATCCCCTATCCGCTGGATGAGGCCGCCATCGATTTCGACGTCCTCGGCTATTCGGCGC
GCAATCCCGAACGCGTCGATGTGCTGTTGGCGGCTTGTCGCAAGGAACACATCGAAGTTCGCGAGGCGGCGCTGGAGCTC
GCCGGGCTGACGGCGCGGGTGGTGGACCTGGAGGTCTGCGCGCTGGAGCGCTCGTTCGCCCTGGCGGCCGGGGCGTCGGT
TGCCCGGACTGTGGCGCTGGTCGATATCGGAGCCACCCTGACCGCCCTGAGCGTTGTGCATGAAGGGCAGATCATCTATT
CCCGCGAGCAGTTGTTTGGTGGGCACCGACTGACCGAGCACATCCAGCGTCGTTACGGGCTGTCGAAAGAAGAGGCCAGC
CTGGCGAAAAAGCAGGGCGGCCTGCCGGACGATTACGCCAGTGAGGTGCTGCAACCCTTTCAGGAGGCGGTGGTGCAGCA
GGTTTCGCGCTCGTTGCAGTTTTTCCAGGCCTCCGGCCCGCAGCGCCAGGTCGACTCTATCCTGTTGGCGGGCGGCAGCG
CCTCGGTTCCGGGACTTGCGCCGTTGATCGAGCAGCGCCTGGGCTTGCCTGTGCTGATCGCCAACCCCTTCGCTGGCATG
AGCATCGGCGGCAAGGTCGACGCCGCGGCCCTGGCCAGCGAGGCTCCATCCTTGATGATCGCCTGTGGGCTGGCCTTGCG
GAGTTTCGACTGA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-349)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	55.114	100	0.554
comM	Acinetobacter nosocomialis M2	54.83	100	0.551
comM	Acinetobacter baylyi ADP1	51.705	100	0.52
pilM	Legionella pneumophila strain ERS1305867	45.198	100	0.457