Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACDH68_RS15675 Genome accession   NZ_CP167868
Coordinates   3632921..3634657 (-) Length   578 a.a.
NCBI ID   WP_071117396.1    Uniprot ID   -
Organism   Xanthomonas axonopodis pv. poinsettiicola strain NCPPB:3948     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3627921..3639657
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACDH68_RS15660 (ACDH68_15660) sucC 3627972..3629141 (-) 1170 WP_007965356.1 ADP-forming succinate--CoA ligase subunit beta -
  ACDH68_RS15665 (ACDH68_15665) - 3629373..3630986 (+) 1614 WP_109290731.1 PAS domain-containing sensor histidine kinase -
  ACDH68_RS15670 (ACDH68_15670) pilR 3631253..3632707 (+) 1455 WP_011348337.1 sigma-54 dependent transcriptional regulator Regulator
  ACDH68_RS15675 (ACDH68_15675) pilB 3632921..3634657 (-) 1737 WP_071117396.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACDH68_RS15680 (ACDH68_15680) - 3634755..3635411 (-) 657 WP_157733690.1 methionine biosynthesis protein MetW -
  ACDH68_RS15685 (ACDH68_15685) - 3635404..3636432 (-) 1029 WP_372356859.1 glycosyltransferase -
  ACDH68_RS15690 (ACDH68_15690) - 3636447..3637241 (-) 795 WP_005915828.1 glycosyltransferase -
  ACDH68_RS15695 (ACDH68_15695) - 3637246..3637746 (-) 501 WP_143030780.1 SMR family transporter -
  ACDH68_RS15700 (ACDH68_15700) - 3637983..3638387 (+) 405 WP_083347868.1 pilin -

Sequence

Protein


Download         Length: 578 a.a.        Molecular weight: 62562.59 Da        Isoelectric Point: 5.5986

>NTDB_id=1040045 ACDH68_RS15675 WP_071117396.1 3632921..3634657(-) (pilB) [Xanthomonas axonopodis pv. poinsettiicola strain NCPPB:3948]
MNSVVTANLAGITGIARRLVQDGALDEGAARAAMDQAATAKVPLPQWFSEKKLVTASQLAAANAVEFGMPLLDVSAFDAN
QNAVKLVSEELLQKHQVLPLFKRGNRLFIGVSNPTQTRALDDIKFHTNLVVEAILVDEDQIRRTLEQWQASNASLGSSLG
NDDEEMGDLDVSAGDEDMGAGGDSGVDAKGDDTPVVKFVNKVLVDAIRRGASDIHFEPYEDDYRVRLRIDGLLKNVAKAP
VKLNQRIAARLKVMSQLDIAEKRVPQDGRIKLNLSKTKQIDFRVSTLPTLFGEKVVLRILDGSAAKLGIDKLGYEADQQK
LFLEAIHKPYGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEIRLPGVNQVQQNNKRGMTFAAALRSFLRQDP
DIIMVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCNNCKRKSTLP
DNALLAEGFTPAQLAAGIELYEAVGCDECTEGYKGRTGIYQVMPMTDEIGAIVLEGGNAMQIAEAAQAIGIRDLRQSALM
KAAHGVTSLAEINRVTKD

Nucleotide


Download         Length: 1737 bp        

>NTDB_id=1040045 ACDH68_RS15675 WP_071117396.1 3632921..3634657(-) (pilB) [Xanthomonas axonopodis pv. poinsettiicola strain NCPPB:3948]
ATGAATAGCGTCGTCACAGCTAACTTGGCTGGTATTACGGGAATCGCCCGGCGTCTTGTGCAGGACGGTGCCTTGGATGA
AGGCGCGGCGCGTGCTGCGATGGATCAGGCCGCTACTGCAAAGGTCCCGTTGCCTCAATGGTTCTCGGAGAAAAAGTTGG
TGACAGCTTCGCAGCTGGCGGCCGCCAATGCAGTCGAGTTCGGCATGCCGCTGCTGGATGTATCGGCGTTCGATGCCAAC
CAGAACGCGGTCAAGCTGGTCAGTGAGGAGCTGCTCCAGAAGCACCAGGTGCTGCCGCTGTTCAAGCGCGGCAACCGGTT
GTTCATAGGGGTGAGCAACCCGACACAGACTCGCGCGCTGGACGACATCAAGTTCCATACGAACTTAGTGGTCGAGGCGA
TCCTCGTCGACGAAGATCAGATCCGCCGGACGCTCGAGCAGTGGCAGGCCAGCAATGCGTCGTTGGGTTCTTCGCTTGGC
AACGACGACGAGGAAATGGGGGATCTGGACGTCTCGGCCGGCGACGAGGACATGGGCGCCGGCGGGGATTCCGGGGTCGA
TGCCAAGGGCGACGACACGCCGGTGGTGAAGTTCGTCAACAAGGTGCTGGTGGATGCGATCAGGCGGGGAGCCTCGGACA
TCCATTTCGAGCCGTATGAAGACGACTACCGGGTGCGCTTGCGCATCGACGGCTTGTTGAAGAACGTGGCGAAGGCGCCG
GTGAAGCTGAACCAGCGCATCGCAGCGCGCTTGAAGGTGATGTCGCAGCTGGATATCGCCGAGAAGCGGGTGCCGCAGGA
CGGGCGCATCAAGCTCAATCTGTCCAAGACCAAGCAGATCGACTTTCGTGTCAGCACCTTGCCGACCCTGTTCGGTGAAA
AGGTGGTGCTGCGTATCCTGGACGGCAGCGCGGCCAAGCTGGGCATCGACAAGCTGGGCTATGAGGCGGACCAGCAGAAG
CTGTTCCTGGAGGCGATCCACAAGCCGTACGGGATGGTGCTGGTGACCGGGCCGACCGGCTCGGGCAAGACGGTGTCGTT
GTATACGGCGCTGGGCATCCTCAACGACGAGACACGCAATATCTCCACCGCGGAGGACCCGGTCGAAATCCGCCTGCCTG
GCGTCAATCAGGTACAGCAGAACAACAAGCGTGGCATGACCTTTGCAGCAGCGCTGCGCTCGTTCCTGCGTCAGGACCCG
GACATCATCATGGTCGGCGAAATCCGTGACCTGGAGACGGCCGAGATTGCGATCAAGGCGGCGCAGACCGGTCACATGGT
GTTGTCGACGTTGCACACCAACGATGCGCCGCAGACCATCGCACGCTTGATGAACATGGGCATCGCGCCCTACAACATTA
CCTCGTCGGTGACCTTGGTGATCGCGCAACGTCTGGCGCGGCGGTTGTGCAACAACTGCAAGCGCAAGTCGACGCTGCCT
GACAACGCCTTGCTGGCCGAAGGATTCACCCCCGCACAGCTTGCTGCCGGGATCGAGCTGTATGAGGCGGTCGGTTGCGA
CGAGTGCACCGAAGGCTACAAGGGGCGTACGGGTATCTACCAGGTCATGCCGATGACCGACGAGATCGGCGCGATCGTGC
TGGAGGGCGGCAATGCGATGCAGATTGCCGAGGCCGCGCAGGCGATCGGTATCCGCGATTTGCGGCAGTCGGCCTTGATG
AAGGCTGCGCACGGGGTGACCAGCCTGGCCGAGATCAATCGGGTGACCAAGGACTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(50-153)

Type II/IV secretion system protein (T2SSE)

(201-468)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

55.986

98.27

0.55
  pilB Acinetobacter baylyi ADP1

55.106

98.27

0.542
  pilB Legionella pneumophila strain ERS1305867

52.548

98.443

0.517
  pilF Neisseria gonorrhoeae MS11

49.472

98.27

0.486
  pilB Vibrio cholerae strain A1552

50

95.848

0.479
  pilB Vibrio parahaemolyticus RIMD 2210633

46.786

96.886

0.453
  pilB Vibrio campbellii strain DS40M4

45.343

98.443

0.446

Multiple sequence alignment