Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACCQ07_RS15940 Genome accession   NZ_CP167844
Coordinates   3675680..3677416 (-) Length   578 a.a.
NCBI ID   WP_372363449.1    Uniprot ID   -
Organism   Xanthomonas sp. NCPPB 3583     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3670680..3682416
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACCQ07_RS15925 (ACCQ07_15925) sucC 3670722..3671891 (-) 1170 WP_057681815.1 ADP-forming succinate--CoA ligase subunit beta -
  ACCQ07_RS15930 (ACCQ07_15930) - 3672123..3673736 (+) 1614 WP_122273517.1 PAS domain-containing sensor histidine kinase -
  ACCQ07_RS15935 (ACCQ07_15935) pilR 3674004..3675458 (+) 1455 WP_011348337.1 sigma-54 dependent transcriptional regulator Regulator
  ACCQ07_RS15940 (ACCQ07_15940) pilB 3675680..3677416 (-) 1737 WP_372363449.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACCQ07_RS15945 (ACCQ07_15945) - 3677451..3678977 (-) 1527 WP_031423872.1 spermidine synthase -
  ACCQ07_RS15950 (ACCQ07_15950) - 3678947..3679576 (-) 630 WP_042773942.1 hypothetical protein -
  ACCQ07_RS15955 (ACCQ07_15955) - 3679587..3681176 (-) 1590 WP_031423877.1 phosphoethanolamine transferase -
  ACCQ07_RS15960 (ACCQ07_15960) pilA2 3681312..3681737 (-) 426 WP_031423879.1 pilin Machinery gene

Sequence

Protein


Download         Length: 578 a.a.        Molecular weight: 62714.80 Da        Isoelectric Point: 5.4526

>NTDB_id=1039780 ACCQ07_RS15940 WP_372363449.1 3675680..3677416(-) (pilB) [Xanthomonas sp. NCPPB 3583]
MNSVATTNLVGITGIARRLVQDGALEEAAARTAMEQAAAAKVPLPQWFAEKRLVSASQLAAANAVEFGMPLMDVSAFDAN
QNAVKLVSEELLQKYQVLPLFKRGNRLFVGVSNPTQTRALDDIKFHTNLVVEPILVDEDQIRRTLEQWQASNASLGSSLG
NDDDEMGDLDVSTGDEDMGAGGDAGVDAKGDDTPVVKFVNKVLVDAIRRGASDIHFEPYEDDYRVRLRIDGLLKNVAKAP
VKLNQRIAARLKVMSQLDIAEKRVPQDGRIKLNLSKTKQIDFRVSTLPTLFGEKVVLRILDGSAAKLGIDKLGYEPDQQK
LFLEAIHKPYGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEIRLPGVNQVQQNNKRGMTFAAALRSFLRQDP
DIIMVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCNNCKRKSTLP
DNALLAEGFTPAQLAAGIELYEAVGCDECTEGYKGRTGIYQVMPMTDEIGAIVLEGGNAMQIAEAAQAIGIRDLRQSALM
KAAHGVTSLAEINRVTKD

Nucleotide


Download         Length: 1737 bp        

>NTDB_id=1039780 ACCQ07_RS15940 WP_372363449.1 3675680..3677416(-) (pilB) [Xanthomonas sp. NCPPB 3583]
ATGAATTCAGTAGCTACCACCAATCTCGTTGGCATCACCGGCATCGCGCGTCGCCTTGTGCAGGATGGCGCCCTGGAGGA
AGCCGCAGCGCGGACGGCGATGGAACAGGCGGCCGCTGCCAAGGTGCCGCTGCCGCAGTGGTTTGCCGAGAAGAGGCTGG
TGTCGGCGTCGCAGCTGGCGGCGGCCAACGCAGTGGAATTCGGCATGCCGCTGATGGATGTGTCTGCGTTCGACGCTAAC
CAGAACGCAGTCAAGCTAGTCAGCGAGGAGTTGCTCCAGAAGTACCAGGTGCTGCCGCTGTTCAAGCGCGGCAATCGGTT
GTTTGTGGGAGTGAGCAACCCGACCCAGACCCGGGCGCTGGACGACATCAAGTTCCACACCAATCTGGTGGTCGAGCCGA
TCCTGGTCGACGAGGACCAGATTCGTCGTACCTTGGAACAGTGGCAGGCCAGCAACGCGTCGTTGGGTTCGTCGCTTGGC
AACGACGACGATGAGATGGGGGATCTGGACGTCTCCACCGGCGACGAGGACATGGGCGCCGGCGGGGATGCCGGGGTCGA
TGCCAAGGGCGACGACACGCCTGTGGTGAAGTTCGTCAACAAGGTGCTGGTGGATGCGATCCGGCGGGGAGCCTCGGACA
TCCATTTCGAGCCCTATGAAGACGATTACCGGGTGCGGTTGCGGATCGATGGGCTGCTCAAGAATGTGGCCAAGGCGCCG
GTGAAGCTGAATCAGCGCATCGCGGCTCGCTTGAAGGTGATGTCGCAGCTGGATATCGCGGAGAAGCGGGTGCCGCAGGA
CGGGCGCATCAAGCTCAACCTGTCCAAGACCAAGCAGATCGACTTCCGCGTCAGCACCTTGCCGACGCTGTTCGGCGAGA
AGGTGGTGCTGCGTATCCTGGACGGCAGCGCGGCCAAGCTGGGCATCGACAAGCTGGGCTACGAGCCGGATCAGCAGAAG
CTGTTCCTGGAGGCGATCCACAAGCCGTACGGGATGGTGCTGGTGACCGGGCCGACTGGTTCGGGCAAGACGGTGTCGTT
GTATACGGCGCTGGGAATTCTCAACGACGAGACGCGCAATATCTCCACCGCGGAGGATCCGGTCGAAATCCGCTTGCCTG
GCGTCAATCAGGTACAGCAGAACAACAAGCGTGGCATGACCTTCGCGGCAGCCTTGCGCTCGTTCCTGCGTCAGGACCCA
GACATCATCATGGTTGGCGAAATCCGTGACCTGGAGACGGCCGAGATTGCGATCAAGGCGGCGCAGACCGGTCACATGGT
GTTGTCGACGTTGCACACCAACGATGCGCCGCAGACCATCGCACGCTTGATGAACATGGGCATCGCGCCCTACAACATTA
CCTCGTCGGTGACCTTGGTGATCGCGCAGCGTCTGGCGCGGCGGTTGTGCAACAACTGCAAGCGCAAGTCGACGCTGCCT
GACAACGCCTTGCTGGCTGAAGGATTCACGCCCGCACAGCTTGCCGCCGGGATCGAGCTGTATGAGGCGGTCGGTTGCGA
CGAGTGCACCGAAGGCTACAAGGGGCGTACCGGTATCTACCAGGTCATGCCGATGACCGACGAGATCGGCGCGATCGTGC
TGGAGGGCGGCAATGCGATGCAGATCGCCGAGGCCGCGCAGGCGATCGGTATCCGCGATTTGCGGCAGTCGGCCTTGATG
AAGGCTGCCCACGGGGTGACCAGCCTGGCCGAGATCAATCGGGTGACCAAGGACTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(201-468)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(49-153)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

55.652

99.481

0.554
  pilB Acinetobacter baumannii D1279779

56.162

98.27

0.552
  pilB Legionella pneumophila strain ERS1305867

52.021

98.443

0.512
  pilB Vibrio cholerae strain A1552

49.306

99.654

0.491
  pilF Neisseria gonorrhoeae MS11

49.472

98.27

0.486
  pilB Vibrio parahaemolyticus RIMD 2210633

46.099

97.578

0.45
  pilB Vibrio campbellii strain DS40M4

45.343

98.443

0.446

Multiple sequence alignment