Detailed information NTG1039432

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: ACCO40_RS12950
• Genome accession: NZ_CP167793
• Coordinates: 2536962..2537579 (-)
• Length: 205 a.a.
• NCBI ID: WP_114150363.1
• Uniprot ID: -
• Organism: Bacillus spizizenii strain SHT-15
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2531962..2542579

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACCO40_RS12920 (ACCO40_12920)	rpsT	2532263..2532529 (+)	267	WP_003229989.1	30S ribosomal protein S20	-
ACCO40_RS12925 (ACCO40_12925)	holA	2532544..2533587 (-)	1044	WP_061186855.1	DNA polymerase III subunit delta	-
ACCO40_RS12930 (ACCO40_12930)	-	2533627..2533776 (-)	150	WP_003229985.1	hypothetical protein	-
ACCO40_RS12935 (ACCO40_12935)	-	2533817..2533951 (+)	135	WP_003229983.1	YqzM family protein	-
ACCO40_RS12940 (ACCO40_12940)	comEC	2533992..2536322 (-)	2331	WP_114150364.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
ACCO40_RS12945 (ACCO40_12945)	-	2536326..2536895 (-)	570	WP_061186853.1	ComE operon protein 2	-
ACCO40_RS12950 (ACCO40_12950)	comEA	2536962..2537579 (-)	618	WP_114150363.1	helix-hairpin-helix domain-containing protein	Machinery gene
ACCO40_RS12955 (ACCO40_12955)	comER	2537663..2538484 (+)	822	WP_014114474.1	late competence protein ComER	-
ACCO40_RS12960 (ACCO40_12960)	-	2538550..2539293 (-)	744	WP_114150362.1	class I SAM-dependent methyltransferase	-
ACCO40_RS12965 (ACCO40_12965)	rsfS	2539290..2539646 (-)	357	WP_003237112.1	ribosome silencing factor	-
ACCO40_RS12970 (ACCO40_12970)	yqeK	2539664..2540224 (-)	561	WP_014114476.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
ACCO40_RS12975 (ACCO40_12975)	-	2540214..2540783 (-)	570	WP_014114477.1	nicotinate-nucleotide adenylyltransferase	-
ACCO40_RS12980 (ACCO40_12980)	yhbY	2540795..2541085 (-)	291	WP_014114478.1	ribosome assembly RNA-binding protein YhbY	-
ACCO40_RS12985 (ACCO40_12985)	aroE	2541079..2541921 (-)	843	WP_072183538.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 205 a.a.
• Molecular weight: 22072.92 Da
• Isoelectric Point: 4.8542

>NTDB_id=1039432 ACCO40_RS12950 WP_114150363.1 2536962..2537579(-) (comEA) [Bacillus spizizenii strain SHT-15]
MNWLNQHKKAIILAASAAVFIAVIFFLSSGKNKEPMKQTVSTETENTEVKQEAFKDESNETIVIDIKGAVKHPGVYEMRT
GDRLSQAIEKAGGTSEQADEMQVNLAELLQDGTVVYIPKRGEETAVQQGVGGAIQSDGGKGALVNINTATLEELQGISGV
GPSKAEAIIAYREENGRYQTIEDITKVSGIGEKSFEKIKSSITVK

Nucleotide

• Length: 618 bp

>NTDB_id=1039432 ACCO40_RS12950 WP_114150363.1 2536962..2537579(-) (comEA) [Bacillus spizizenii strain SHT-15]
ATGAATTGGTTGAATCAGCATAAGAAAGCAATTATTTTAGCGGCTTCTGCGGCTGTTTTCATAGCGGTTATTTTCTTCCT
GTCCAGTGGGAAAAACAAAGAGCCGATGAAGCAGACTGTATCAACTGAGACAGAAAATACGGAGGTAAAGCAGGAAGCAT
TCAAAGACGAGTCAAACGAAACGATTGTGATTGACATCAAAGGTGCTGTTAAGCATCCTGGTGTTTATGAAATGCGAACA
GGGGACAGATTATCTCAGGCGATTGAGAAAGCCGGCGGGACCAGTGAACAAGCAGACGAAATGCAAGTGAATTTGGCGGA
GCTGCTGCAGGACGGGACAGTGGTGTACATCCCGAAAAGGGGTGAGGAGACAGCGGTGCAGCAAGGAGTCGGAGGCGCCA
TCCAAAGTGATGGAGGGAAGGGGGCGCTGGTAAATATCAATACTGCAACCTTGGAGGAATTACAAGGCATCTCAGGGGTG
GGGCCATCCAAAGCTGAAGCTATTATTGCATACCGGGAAGAAAATGGGCGTTACCAGACAATTGAAGATATCACGAAGGT
TTCAGGAATCGGTGAAAAGTCGTTTGAGAAAATAAAGTCTTCCATTACAGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (142-203)

• SLBB domain (SLBB) (63-118)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	89.756	100	0.898
comEA	Staphylococcus aureus MW2	36.866	100	0.39
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	36.792	100	0.38
comEA	Staphylococcus aureus N315	34.562	100	0.366