Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACCP97_RS15975 Genome accession   NZ_CP167224
Coordinates   3668476..3670212 (-) Length   578 a.a.
NCBI ID   WP_218557137.1    Uniprot ID   -
Organism   Xanthomonas campestris pv. fici strain NCPPB 3797     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3663476..3675212
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACCP97_RS15960 (ACCP97_15960) - 3664515..3666128 (+) 1614 WP_122273517.1 PAS domain-containing sensor histidine kinase -
  ACCP97_RS15965 (ACCP97_15965) pilR 3666396..3667850 (+) 1455 WP_011348337.1 sigma-54 dependent transcriptional regulator Regulator
  ACCP97_RS15970 (ACCP97_15970) pilB 3668079..3668198 (-) 120 WP_136732873.1 pilus assembly protein Machinery gene
  ACCP97_RS15975 (ACCP97_15975) pilB 3668476..3670212 (-) 1737 WP_218557137.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACCP97_RS15980 (ACCP97_15980) - 3670247..3671773 (-) 1527 WP_218557136.1 spermidine synthase -
  ACCP97_RS15985 (ACCP97_15985) - 3671743..3672369 (-) 627 WP_228737286.1 hypothetical protein -
  ACCP97_RS15990 (ACCP97_15990) - 3672383..3673972 (-) 1590 WP_031423877.1 phosphoethanolamine transferase -
  ACCP97_RS15995 (ACCP97_15995) pilA2 3674108..3674533 (-) 426 WP_031423879.1 pilin Machinery gene

Sequence

Protein


Download         Length: 578 a.a.        Molecular weight: 62718.79 Da        Isoelectric Point: 5.4549

>NTDB_id=1038966 ACCP97_RS15975 WP_218557137.1 3668476..3670212(-) (pilB) [Xanthomonas campestris pv. fici strain NCPPB 3797]
MNSVATTNLVGITGIARRLVQDGALEEAAARTAMEQAAAAKVPLPQWFAEKRLVSASQLAAANAVEFGMPLMDVSAFDAN
QNAVKLVSEELLQKYQVLPLFKRGNRLFVGVSNPTQTRALDDIKFHTNLVVEPILVDEDQIRRTLEQWQASNASLGSSLG
NDDEEMGDLDVSTGDEDMGAGGDSGVDAKGDDTPVVKFVNKVLVDAIRRGASDIHFEPYEDDYRVRLRIDGLLKNVAKAP
VKLNQRIAARLKVMSQLDIAEKRVPQDGRIKLNLSKTKQIDFRVSTLPTLFGEKVVLRILDGSAAKLGIDKLGYEADQQK
LFLEAIHKPYGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEIRLPGVNQVQQNNKRGMTFAAALRSFLRQDP
DIIMVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCNNCKRKSTLP
DNALLAEGFTPAQLAAGIELYEAVGCDECTEGYKGRTGIYQVMPMTDEIGAIVLEGGNAMQIAEAAQAIGIRDLRQSALM
KAAHGVTSLAEINRVTKD

Nucleotide


Download         Length: 1737 bp        

>NTDB_id=1038966 ACCP97_RS15975 WP_218557137.1 3668476..3670212(-) (pilB) [Xanthomonas campestris pv. fici strain NCPPB 3797]
ATGAATTCAGTAGCTACCACCAATCTCGTTGGCATCACCGGCATCGCGCGTCGCCTTGTGCAGGATGGCGCCCTGGAGGA
AGCCGCAGCGCGGACGGCGATGGAACAGGCGGCCGCTGCCAAGGTGCCGCTGCCGCAGTGGTTTGCCGAGAAGAGGCTGG
TGTCGGCGTCGCAGCTGGCGGCGGCCAACGCAGTGGAATTCGGCATGCCGCTGATGGATGTGTCTGCGTTCGACGCTAAC
CAGAACGCAGTCAAGCTAGTCAGCGAGGAGTTGCTCCAGAAGTACCAGGTGCTGCCGCTGTTCAAGCGCGGCAATCGGTT
GTTTGTGGGAGTGAGCAACCCGACCCAGACCCGGGCGCTGGACGACATCAAGTTCCACACCAATCTGGTGGTCGAGCCGA
TCCTGGTCGACGAGGACCAGATTCGTCGTACCTTGGAACAGTGGCAGGCCAGCAACGCGTCGTTGGGTTCGTCGCTTGGC
AACGACGACGAAGAAATGGGAGATCTGGACGTCTCCACCGGCGACGAGGACATGGGCGCCGGCGGGGATTCCGGGGTCGA
TGCCAAGGGCGACGACACGCCGGTGGTGAAGTTCGTCAACAAGGTGCTGGTGGATGCGATCAGGCGGGGAGCCTCGGACA
TCCATTTCGAGCCGTATGAAGACGACTACCGGGTGCGCTTGCGCATCGACGGCTTGTTGAAGAATGTGGCGAAGGCGCCG
GTGAAGCTGAACCAGCGCATCGCAGCGCGCTTGAAGGTGATGTCGCAGCTGGATATCGCCGAGAAGCGGGTGCCGCAGGA
CGGGCGCATCAAGCTCAACCTGTCCAAGACCAAGCAGATCGACTTTCGTGTCAGCACCTTGCCGACCCTGTTCGGTGAAA
AAGTGGTGCTGCGTATCCTGGACGGCAGCGCGGCCAAGCTGGGCATCGACAAGCTGGGCTATGAGGCGGACCAGCAGAAG
CTGTTCCTGGAGGCGATCCACAAGCCGTACGGGATGGTGCTGGTGACCGGGCCGACCGGCTCGGGCAAGACGGTGTCGTT
GTACACGGCGCTGGGCATCCTCAACGACGAGACACGCAATATCTCCACCGCGGAGGACCCGGTTGAAATCCGCTTGCCTG
GCGTCAATCAGGTGCAGCAGAACAACAAGCGTGGCATGACCTTTGCCGCAGCGTTGCGCTCGTTCCTACGCCAGGACCCA
GACATCATCATGGTCGGCGAAATCCGTGACCTGGAGACGGCCGAGATTGCGATCAAGGCGGCGCAGACCGGTCACATGGT
GTTGTCGACGTTGCACACCAACGATGCGCCGCAGACCATCGCACGCTTGATGAACATGGGCATCGCGCCCTACAACATTA
CCTCGTCGGTGACCTTGGTGATCGCGCAGCGTCTGGCGCGGCGGTTGTGCAACAACTGCAAGCGCAAGTCGACGCTGCCT
GACAACGCCTTGCTGGCCGAAGGATTCACGCCCGCACAGCTTGCCGCCGGGATCGAGCTGTACGAGGCGGTCGGTTGCGA
CGAGTGCACCGAAGGCTACAAGGGGCGTACCGGTATCTACCAGGTCATGCCGATGACCGACGAGATCGGCGCGATCGTGC
TGGAGGGCGGCAATGCGATGCAGATTGCCGAGGCCGCGCAGGCGATCGGTATCCGCGATTTGCGGCAGTCGGCCTTGATG
AAGGCTGCCCACGGGGTTACCAGCCTGGCCGAGATCAATCGTGTAACGAAGGACTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(201-468)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(49-153)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

56.514

98.27

0.555
  pilB Acinetobacter baylyi ADP1

55.478

99.481

0.552
  pilB Legionella pneumophila strain ERS1305867

51.845

98.443

0.51
  pilB Vibrio cholerae strain A1552

49.827

100

0.498
  pilF Neisseria gonorrhoeae MS11

49.472

98.27

0.486
  pilB Vibrio parahaemolyticus RIMD 2210633

46.099

97.578

0.45
  pilB Vibrio campbellii strain DS40M4

45.343

98.443

0.446
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.509

91.522

0.362

Multiple sequence alignment