Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   AB8618_RS13245 Genome accession   NZ_CP166996
Coordinates   2761729..2762865 (-) Length   378 a.a.
NCBI ID   WP_413400782.1    Uniprot ID   -
Organism   Pseudoalteromonas sp. RW-H-Ap-1     
Function   mediate the depolymerization of the type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2756729..2767865
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AB8618_RS13215 (AB8618_13200) - 2757108..2758001 (+) 894 WP_010391581.1 hypothetical protein -
  AB8618_RS13220 (AB8618_13205) - 2758079..2758552 (+) 474 WP_010391582.1 hypothetical protein -
  AB8618_RS13225 (AB8618_13210) rsmE 2758623..2759356 (+) 734 Protein_2541 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  AB8618_RS13230 (AB8618_13215) gshB 2759423..2760373 (+) 951 WP_010391584.1 glutathione synthase -
  AB8618_RS13235 (AB8618_13220) - 2760501..2761058 (+) 558 WP_010391585.1 YqgE/AlgH family protein -
  AB8618_RS13240 (AB8618_13225) ruvX 2761076..2761525 (+) 450 WP_010391588.1 Holliday junction resolvase RuvX -
  AB8618_RS13245 (AB8618_13230) pilU 2761729..2762865 (-) 1137 WP_413400782.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  AB8618_RS13250 (AB8618_13235) pilT 2762875..2763915 (-) 1041 WP_010391590.1 type IV pilus twitching motility protein PilT Machinery gene
  AB8618_RS13255 (AB8618_13240) - 2763918..2764631 (+) 714 WP_413400783.1 YggS family pyridoxal phosphate-dependent enzyme -
  AB8618_RS13260 (AB8618_13245) proC 2764649..2765470 (+) 822 WP_138609266.1 pyrroline-5-carboxylate reductase -
  AB8618_RS13265 (AB8618_13250) - 2765497..2766030 (+) 534 WP_008113334.1 YggT family protein -
  AB8618_RS13270 (AB8618_13255) - 2766089..2766532 (+) 444 WP_413400784.1 DUF4426 domain-containing protein -
  AB8618_RS13275 (AB8618_13260) - 2766535..2767134 (+) 600 WP_413400785.1 XTP/dITP diphosphatase -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 41947.95 Da        Isoelectric Point: 6.2895

>NTDB_id=1036667 AB8618_RS13245 WP_413400782.1 2761729..2762865(-) (pilU) [Pseudoalteromonas sp. RW-H-Ap-1]
MTLSLNHFLQIMIDKKGSDLFVSSQLPISAKINGELTPLNDDKLTDEQALELVESAMSEKQKAEFHNTKECNFAIATSEG
RFRISAFWQRDRAGMVIRRIVTQIPDVKELGLPSALTDVIMSKRGLVLFVGGTGTGKSTSLAALIGYRNRNQRGHILTIE
DPIEFVHEHKKSIITQREVGLDTESFESALKSSLRQAPDVILIGEIRSQETMEYALSFAETGHLCVATLHANNANQAIDR
IMHLVPKEKHDKLKYDLALNLRAIIAQQLIPTSDGEGRAAAIEVLLNTPMVSELIKNGNIGGIKETMAKSTEMGMQTFDQ
ALFELYRQQRINYADALHHADSPNDLRLMIKLRNNEQQGAGFLQGVTIDGLDDKGNIT

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=1036667 AB8618_RS13245 WP_413400782.1 2761729..2762865(-) (pilU) [Pseudoalteromonas sp. RW-H-Ap-1]
ATGACTTTATCTCTAAATCACTTTTTACAAATAATGATCGACAAAAAAGGCTCTGATTTATTTGTATCAAGCCAGCTGCC
TATCAGCGCTAAAATTAATGGTGAGCTCACACCTCTTAATGATGACAAACTAACCGATGAGCAAGCGCTGGAATTAGTTG
AATCAGCGATGAGTGAAAAGCAAAAAGCAGAGTTTCATAACACTAAAGAGTGTAACTTTGCGATTGCAACCAGTGAAGGG
CGCTTTCGTATTTCGGCTTTTTGGCAACGCGACAGAGCGGGGATGGTTATTCGCCGGATTGTGACCCAAATACCTGATGT
AAAAGAACTTGGATTGCCCTCAGCGCTTACTGATGTGATTATGTCAAAGCGCGGACTGGTATTATTTGTCGGTGGTACGG
GAACAGGTAAATCAACCTCTTTGGCGGCGCTTATTGGTTATAGAAATCGTAATCAACGAGGGCATATTTTAACTATAGAA
GATCCCATTGAGTTTGTGCATGAACATAAAAAAAGCATTATTACTCAACGTGAAGTGGGCTTAGATACTGAAAGTTTTGA
GTCAGCACTCAAAAGCTCTTTACGACAAGCGCCTGATGTTATTTTAATTGGTGAAATTCGATCGCAAGAAACTATGGAGT
ATGCGTTAAGCTTTGCAGAAACAGGTCATCTATGTGTTGCCACCTTGCATGCTAACAATGCTAACCAAGCTATTGATCGT
ATTATGCATTTAGTGCCAAAAGAGAAGCACGATAAGCTTAAATATGATTTAGCATTAAACTTACGCGCTATTATTGCCCA
GCAGCTTATCCCAACCTCTGATGGGGAGGGACGTGCTGCTGCTATAGAGGTATTATTGAATACGCCAATGGTGAGTGAGC
TGATTAAAAACGGTAACATTGGTGGTATTAAAGAAACTATGGCCAAATCAACTGAAATGGGCATGCAGACCTTTGACCAA
GCGCTGTTTGAGCTTTACAGACAGCAACGGATTAACTATGCCGATGCACTTCATCATGCAGACTCGCCAAATGATTTACG
TTTAATGATAAAACTGCGTAATAACGAGCAGCAAGGGGCAGGCTTTTTGCAAGGTGTAACCATTGATGGCCTTGATGATA
AAGGTAATATTACCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(14-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Acinetobacter baylyi ADP1

57.294

99.735

0.571
  pilU Pseudomonas stutzeri DSM 10701

57.333

99.206

0.569
  pilU Vibrio cholerae strain A1552

56.403

97.09

0.548
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.944

95.238

0.399

Multiple sequence alignment