Detailed information NTG1036269

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: AB9V62_RS19315
• Genome accession: NZ_CP166923
• Coordinates: 4003763..4004908 (-)
• Length: 381 a.a.
• NCBI ID: WP_206408660.1
• Uniprot ID: -
• Organism: Pseudomonas oleovorans strain ERA
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3998763..4009908

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
AB9V62_RS19295 (AB9V62_19295)	ntrC	3999409..4000842 (+)	1434	WP_206408662.1	nitrogen regulation protein NR(I)	-
AB9V62_RS19300 (AB9V62_19300)	-	4001153..4001551 (-)	399	WP_021490398.1	TM2 domain-containing protein	-
AB9V62_RS19305 (AB9V62_19305)	-	4001699..4002910 (+)	1212	WP_003463930.1	putative DNA modification/repair radical SAM protein	-
AB9V62_RS19310 (AB9V62_19310)	-	4002910..4003797 (+)	888	WP_206408661.1	TIGR03915 family putative DNA repair protein	-
AB9V62_RS19315 (AB9V62_19315)	pilU	4003763..4004908 (-)	1146	WP_206408660.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
AB9V62_RS19320 (AB9V62_19320)	pilT	4005074..4006108 (-)	1035	WP_003463935.1	type IV pilus twitching motility protein PilT	Machinery gene
AB9V62_RS19325 (AB9V62_19325)	-	4006184..4006876 (+)	693	WP_206408659.1	YggS family pyridoxal phosphate-dependent enzyme	-
AB9V62_RS19330 (AB9V62_19330)	proC	4006894..4007712 (+)	819	WP_206408658.1	pyrroline-5-carboxylate reductase	-
AB9V62_RS19335 (AB9V62_19335)	-	4007723..4008316 (+)	594	WP_206408657.1	YggT family protein	-
AB9V62_RS19340 (AB9V62_19340)	-	4008398..4008703 (+)	306	WP_074855386.1	DUF167 domain-containing protein	-

Sequence

Protein

• Length: 381 a.a.
• Molecular weight: 42464.89 Da
• Isoelectric Point: 6.7729

>NTDB_id=1036269 AB9V62_RS19315 WP_206408660.1 4003763..4004908(-) (pilU) [Pseudomonas oleovorans strain ERA]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGKIMPVTKTAMSPEMTRETVLGVMNEAQRREFAEKHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTIDDLKLPEILKKLALTKRGLVLFVGATGTGKSTSLAAMIGYRNRNSSGHIISIED
PIEYIHQHQSCIVTQREVGIDTESFEVALKNTLRQAPDVILIGEVRTRETMDHAVAFAETGHLCLATLHANNANQALDRI
INFFPADRQNQVWMDLSLNLKAIVAQQLIPTPDGKGRRAVIEVLINTPLAADLIRKGEVHELKGLMKRSTEQGMQTFDQA
LYNLYTQGEITYEDALLYADSANDLRLMIKLGSETDGDHLTSMAQGLALEVSEEDPGRRFR

Nucleotide

• Length: 1146 bp

>NTDB_id=1036269 AB9V62_RS19315 WP_206408660.1 4003763..4004908(-) (pilU) [Pseudomonas oleovorans strain ERA]
ATGGAATTCGAAAAACTACTGCGTCTGATGGTCGAAAAAGGCGGCTCCGATCTGTTCATCACCGCTGGCGTGCCGCCGTC
GATGAAGGTCAATGGCAAGATCATGCCGGTGACCAAGACCGCCATGTCGCCGGAAATGACCCGCGAAACCGTGCTGGGGG
TGATGAACGAGGCACAGCGTCGCGAGTTTGCCGAAAAGCACGAATGCAACTTCGCCATCAGCGCCCGTGGCATCGGTCGC
TTCCGTGTCAGCGCCTTCTACCAGCGCAACCTGGTGGGCATGGTGCTGCGCCGCATCGAGACCAACATTCCCACCATCGA
CGACCTCAAGCTGCCGGAAATTCTCAAGAAACTGGCGCTGACCAAGCGCGGCCTGGTGCTGTTCGTCGGTGCCACCGGTA
CCGGTAAGTCCACTTCGCTGGCGGCAATGATCGGCTACCGCAACAGGAACAGCAGCGGTCATATCATCTCCATCGAAGAT
CCGATCGAGTACATCCACCAGCACCAGAGCTGCATCGTCACTCAGCGTGAAGTGGGTATCGACACCGAGTCGTTCGAAGT
GGCGCTGAAGAACACGCTGCGTCAGGCGCCGGACGTGATCCTCATCGGCGAGGTGCGCACCCGCGAGACCATGGATCATG
CCGTGGCTTTCGCCGAAACCGGTCACCTGTGTCTGGCCACCCTGCACGCCAACAACGCCAACCAGGCGCTGGACCGCATC
ATCAACTTCTTCCCGGCCGACCGGCAGAACCAGGTGTGGATGGACCTGTCGCTCAACCTCAAGGCCATTGTCGCTCAGCA
GCTGATTCCAACCCCAGATGGTAAAGGTCGTCGCGCGGTGATCGAGGTCCTGATCAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTCAAGGGCCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACAACCTCTACACCCAGGGCGAGATCACCTACGAAGATGCGTTGCTCTACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAACCGATGGCGATCACCTGACCAGCATGGCTCAGGGGTTGGCGCTGGAGGTCAGCGAGG
AAGATCCGGGGCGTCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (110-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	92.388	100	0.924
pilU	Acinetobacter baylyi ADP1	65.266	93.701	0.612
pilU	Vibrio cholerae strain A1552	59.143	91.864	0.543
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.75	88.189	0.386