Detailed information NTG1035885

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: ACAW63_RS20350
• Genome accession: NZ_CP166873
• Coordinates: 4452392..4453618 (-)
• Length: 408 a.a.
• NCBI ID: WP_371228161.1
• Uniprot ID: -
• Organism: Pseudomonas sp. QE6
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4447392..4458618

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACAW63_RS20335 (ACAW63_20330)	-	4448632..4449450 (+)	819	WP_331319522.1	helix-turn-helix transcriptional regulator	-
ACAW63_RS20340 (ACAW63_20335)	-	4449570..4450454 (-)	885	WP_371228157.1	DMT family transporter	-
ACAW63_RS20345 (ACAW63_20340)	-	4450937..4452187 (+)	1251	WP_371228159.1	OprD family porin	-
ACAW63_RS20350 (ACAW63_20345)	pilU	4452392..4453618 (-)	1227	WP_371228161.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
ACAW63_RS20355 (ACAW63_20350)	-	4453806..4454084 (+)	279	WP_152223913.1	peptidylprolyl isomerase	-
ACAW63_RS20360 (ACAW63_20355)	-	4454092..4454457 (+)	366	WP_371228163.1	MmcQ/YjbR family DNA-binding protein	-
ACAW63_RS20365 (ACAW63_20360)	-	4454454..4455008 (+)	555	WP_371228165.1	putative glycolipid-binding domain-containing protein	-
ACAW63_RS20370 (ACAW63_20365)	-	4455343..4456524 (+)	1182	WP_371228167.1	MFS transporter	-
ACAW63_RS20375 (ACAW63_20370)	-	4456787..4458064 (+)	1278	WP_371228169.1	OprD family porin	-

Sequence

Protein

• Length: 408 a.a.
• Molecular weight: 44486.70 Da
• Isoelectric Point: 5.9218

>NTDB_id=1035885 ACAW63_RS20350 WP_371228161.1 4452392..4453618(-) (pilU) [Pseudomonas sp. QE6]
MDIQSMLKILATQDGSDLYLSTGAPPCAKFNGVLKPLSNEPLKAGDVARIGYGLMDEEQRAEFERELEMNLAISVQGVGR
FRINLFKQRNEVSIVARNIKLDIPLFEDLKLPEVLLKVVMEKRGLVLFVGGTGSGKSTSLAALIDHRNRNSGGHIITIED
PIEYVHRHRKSIINQREVGVDTRSFHAALKNTLRQAPDVILIGEIRDRETMEHALAFADTGHLAISTLHANNANQALDRI
INFFPEERRPQLLNDLGNNLKAFVSQRLVRTQDGKRRAAVEVLLGTATISDLIKRGDFSSIKEIMDKSRALGMQTFDQAL
FDLAVEGAISEEEAVKNADSANNLRLKLKLYKDNPGMVTNSAAATAAASPAPAPAAAPAPAADSSSWGLELALEEIEAED
AEGKPAGQ

Nucleotide

• Length: 1227 bp

>NTDB_id=1035885 ACAW63_RS20350 WP_371228161.1 4452392..4453618(-) (pilU) [Pseudomonas sp. QE6]
ATGGATATCCAGTCGATGCTGAAGATCCTGGCCACCCAGGATGGTTCCGACCTCTACCTGTCCACCGGCGCGCCGCCCTG
CGCCAAGTTCAACGGCGTGCTCAAGCCGCTGTCCAACGAGCCTCTCAAGGCGGGCGACGTGGCGCGCATCGGCTACGGCT
TGATGGACGAGGAACAGCGCGCCGAGTTCGAGCGGGAACTGGAGATGAACCTGGCCATTTCGGTGCAGGGTGTCGGCCGC
TTCCGCATCAACCTGTTCAAGCAGCGCAACGAAGTGTCCATCGTGGCGCGCAACATCAAGCTGGACATCCCGCTGTTCGA
GGACCTGAAGCTGCCGGAAGTGCTGCTCAAGGTGGTGATGGAGAAGCGCGGCCTGGTGCTCTTCGTCGGTGGCACCGGCT
CGGGCAAGTCCACCTCCCTGGCGGCGCTGATCGACCACCGCAACCGCAACAGCGGCGGCCACATCATCACCATCGAGGAT
CCCATCGAGTACGTGCATCGGCACCGCAAGTCGATCATCAACCAGCGTGAAGTGGGCGTGGACACCCGCAGCTTCCACGC
CGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCCTGATCGGCGAGATCCGCGACCGCGAGACCATGGAACACG
CCCTGGCCTTCGCCGACACCGGGCACCTGGCGATCTCCACCCTGCACGCGAACAACGCCAACCAGGCGCTGGACCGCATC
ATCAACTTCTTCCCCGAGGAGCGCCGACCGCAGTTGCTCAACGACCTGGGCAACAACCTCAAGGCCTTCGTTTCCCAGCG
CCTGGTGCGCACCCAGGACGGCAAGCGCCGCGCAGCGGTGGAAGTGCTGCTGGGCACCGCCACCATCAGCGACCTGATCA
AGCGCGGCGACTTCAGCTCGATCAAGGAAATCATGGACAAGTCCCGCGCCCTGGGCATGCAGACCTTCGACCAGGCGCTG
TTCGACCTGGCGGTGGAGGGGGCGATCAGCGAAGAAGAAGCGGTGAAGAACGCCGATTCGGCGAACAACCTGCGCCTGAA
GCTCAAGCTGTACAAGGACAACCCGGGCATGGTGACCAACTCCGCCGCAGCCACTGCCGCCGCCAGCCCCGCGCCGGCAC
CCGCCGCAGCGCCTGCACCGGCAGCGGACTCCTCCAGTTGGGGGCTGGAACTGGCGCTGGAAGAGATCGAGGCCGAAGAC
GCGGAAGGCAAGCCGGCTGGGCAATAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (112-271)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	56.303	87.5	0.493
pilU	Acinetobacter baylyi ADP1	53.693	86.275	0.463
pilU	Vibrio cholerae strain A1552	52.286	85.784	0.449
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	40.206	95.098	0.382