Detailed information NTG1035869

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: ACAW63_RS06890
• Genome accession: NZ_CP166873
• Coordinates: 1517785..1518930 (+)
• Length: 381 a.a.
• NCBI ID: WP_371232617.1
• Uniprot ID: -
• Organism: Pseudomonas sp. QE6
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1512785..1523930

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACAW63_RS06865 (ACAW63_06860)	-	1514081..1514371 (-)	291	WP_371232610.1	DUF167 domain-containing protein	-
ACAW63_RS06870 (ACAW63_06865)	-	1514423..1515016 (-)	594	WP_275609792.1	YggT family protein	-
ACAW63_RS06875 (ACAW63_06870)	proC	1515030..1515863 (-)	834	WP_371232613.1	pyrroline-5-carboxylate reductase	-
ACAW63_RS06880 (ACAW63_06875)	-	1515881..1516570 (-)	690	WP_371232614.1	YggS family pyridoxal phosphate-dependent enzyme	-
ACAW63_RS06885 (ACAW63_06880)	pilT	1516644..1517678 (+)	1035	WP_151189058.1	type IV pilus twitching motility protein PilT	Machinery gene
ACAW63_RS06890 (ACAW63_06885)	pilU	1517785..1518930 (+)	1146	WP_371232617.1	type IV pilus ATPase PilU	Machinery gene
ACAW63_RS06895 (ACAW63_06890)	-	1519023..1519427 (+)	405	WP_371232619.1	NINE protein	-
ACAW63_RS06900 (ACAW63_06895)	-	1519653..1521026 (+)	1374	WP_371232621.1	pyridoxal-phosphate dependent enzyme	-
ACAW63_RS06905 (ACAW63_06900)	-	1521023..1522192 (+)	1170	WP_371232622.1	cystathionine gamma-synthase	-
ACAW63_RS06910 (ACAW63_06905)	-	1522371..1523642 (-)	1272	WP_371232623.1	dihydroorotase	-

Sequence

Protein

• Length: 381 a.a.
• Molecular weight: 42590.87 Da
• Isoelectric Point: 6.7258

>NTDB_id=1035869 ACAW63_RS06890 WP_371232617.1 1517785..1518930(+) (pilU) [Pseudomonas sp. QE6]
MEFEKLLRLMVEKGASDLFITAGVPPSMKVNGKVLPVTKTALSPEQTRETVLSVMNEQQRREFAENHECNFAISARGVGR
FRVSAFYQRNLVGMVLRRIETNIPTFEDLKLPEVLKKLAMTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYSQGEITYEDALAYADSANDLRLMIKLGSETDADHLSTMTQGLTLEMSDDDPNRRFR

Nucleotide

• Length: 1146 bp

>NTDB_id=1035869 ACAW63_RS06890 WP_371232617.1 1517785..1518930(+) (pilU) [Pseudomonas sp. QE6]
ATGGAATTCGAGAAGCTGTTGCGCCTCATGGTGGAAAAGGGTGCCTCCGACCTCTTCATCACGGCGGGCGTGCCGCCTTC
GATGAAGGTCAACGGCAAGGTGCTGCCGGTGACCAAGACCGCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTCTCGG
TGATGAACGAGCAGCAGCGCCGCGAGTTCGCCGAGAACCACGAGTGCAACTTCGCCATCAGCGCCCGCGGCGTCGGCCGC
TTCCGCGTCAGCGCCTTCTACCAGCGCAACCTGGTGGGCATGGTGCTGCGCCGCATCGAGACCAACATCCCGACCTTCGA
GGACCTCAAGCTGCCGGAAGTCCTCAAGAAGCTGGCGATGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGTA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGCCACATCATCTCCATCGAAGAC
CCGATCGAATACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGATACCGATTCCTTCGAGGT
GGCCCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGCGAGACCATGGACCACG
CGGTGGCCTTCGCCGAAACCGGCCACCTGTGCCTGGCGACCCTGCACGCCAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGGCACGGCCAGGTGTGGATGGACCTGTCGCTCAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTACCGACCCCTGACGGCAAGGGCCGCCGCGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAAGTGCACGAGCTCAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACAGCCAGGGCGAGATCACCTACGAAGATGCGCTGGCCTACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTGGGCTCGGAAACCGACGCCGATCATCTCTCCACCATGACCCAGGGGCTGACCCTGGAAATGAGCGACG
ACGACCCCAACCGCCGTTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.976	100	0.89
pilU	Acinetobacter baylyi ADP1	63.564	98.688	0.627
pilU	Vibrio cholerae strain A1552	59.299	97.375	0.577
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.976	0.383