Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   ABVF47_RS07135 Genome accession   NZ_CP166869
Coordinates   1556158..1557837 (+) Length   559 a.a.
NCBI ID   WP_371687817.1    Uniprot ID   -
Organism   Snodgrassella alvi strain Acer06     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1551158..1562837
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABVF47_RS07120 (ABVF47_007120) - 1551475..1553241 (+) 1767 WP_371687814.1 M61 family metallopeptidase -
  ABVF47_RS07125 (ABVF47_007125) - 1553290..1554264 (+) 975 WP_371687815.1 hypothetical protein -
  ABVF47_RS07130 (ABVF47_007130) recJ 1554261..1555958 (+) 1698 WP_371687816.1 single-stranded-DNA-specific exonuclease RecJ -
  ABVF47_RS07135 (ABVF47_007135) pilF 1556158..1557837 (+) 1680 WP_371687817.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ABVF47_RS07140 (ABVF47_007140) pilG 1557887..1559119 (+) 1233 WP_370469868.1 type II secretion system F family protein Machinery gene
  ABVF47_RS07145 (ABVF47_007145) pilD 1559126..1559977 (+) 852 WP_370469867.1 A24 family peptidase Machinery gene
  ABVF47_RS07150 (ABVF47_007150) coaE 1559974..1560588 (+) 615 WP_367421119.1 dephospho-CoA kinase -
  ABVF47_RS07155 (ABVF47_007155) zapD 1560594..1561346 (+) 753 WP_100140881.1 cell division protein ZapD -
  ABVF47_RS07160 (ABVF47_007160) yacG 1561346..1561543 (+) 198 WP_100140841.1 DNA gyrase inhibitor YacG -
  ABVF47_RS07165 (ABVF47_007165) - 1561592..1562509 (+) 918 WP_370469866.1 ABC transporter ATP-binding protein -

Sequence

Protein


Download         Length: 559 a.a.        Molecular weight: 62239.81 Da        Isoelectric Point: 5.7880

>NTDB_id=1035708 ABVF47_RS07135 WP_371687817.1 1556158..1557837(+) (pilF) [Snodgrassella alvi strain Acer06]
MSVGLIRVLYKHQIIDAEKAEKLRQELEQKRPVIPILLQSGVIDADKLATFLSKVFSIPYLNLELYNQSLVPQDLINLKI
MQQYICVPIFKRGNRLFLAVSDPTSIQHYQKIATDAGCILNLVIVRDDQLRQILENLGQESSSLLEGMDTVGDAEATGNN
AMSADDEDSENGPVARFVFKVLNDALNSGASDIHFEFYEEMARVRFRIDGQLREVVRMPLNIRNQIASRIKVMAKLDISE
KRIPQDGRIQIQFRKGAKPIDFRVSTLPTLFGEKIVMRILNSDAATLNIEQLGFEPFQKELLLEAIHRPYGMVLVTGPTG
SGKTVSLYTCLNILNTESVNISTAEDPAEINLPGINQVNVNDKQGLTFAAALKSFLRQDPDIIMVGEIRDLETADIAIKA
AQTGHMVFSTLHTNNAPATLSRMLNMGVAPFNIASSVNLIMAQRLVRRLCTCKAPAERPPEYAMKKAGFSDEDLAKDWTF
YRPVGCERCKGKGYKGRAGVYEVMPVSEEMQRIIMNNGNEVDISNQALKEGLVDLRHAGLLKVMEGITSLDEILATTND

Nucleotide


Download         Length: 1680 bp        

>NTDB_id=1035708 ABVF47_RS07135 WP_371687817.1 1556158..1557837(+) (pilF) [Snodgrassella alvi strain Acer06]
ATGAGTGTAGGATTGATTCGAGTTTTATATAAGCACCAGATTATCGATGCAGAAAAAGCTGAAAAACTGCGTCAGGAGCT
TGAGCAGAAACGTCCAGTTATACCGATACTGTTGCAGTCTGGTGTCATCGATGCTGACAAACTGGCCACCTTCCTGTCCA
AGGTATTCAGTATTCCTTACCTCAATCTGGAACTATATAACCAAAGCCTTGTACCGCAGGACTTAATCAATCTGAAAATC
ATGCAGCAATATATCTGCGTGCCCATTTTCAAACGTGGAAATCGTCTGTTTCTGGCTGTATCTGACCCCACTTCCATTCA
GCATTATCAGAAAATTGCCACAGATGCCGGCTGCATACTGAATCTGGTCATTGTACGCGATGATCAGCTGCGTCAGATAC
TCGAAAATCTCGGACAGGAATCCTCCAGCCTACTAGAAGGAATGGACACAGTTGGTGATGCTGAAGCAACAGGCAATAAT
GCCATGTCTGCCGATGATGAAGACAGTGAAAATGGGCCTGTTGCCAGATTTGTATTCAAAGTACTGAATGATGCTCTGAA
CAGCGGCGCTTCAGATATACACTTCGAATTTTACGAAGAAATGGCACGAGTACGTTTTCGCATCGATGGTCAGTTACGTG
AAGTAGTGCGTATGCCACTGAATATTCGAAACCAGATTGCCTCCCGTATTAAAGTAATGGCTAAACTGGACATTTCAGAA
AAACGTATACCCCAAGATGGCCGAATTCAGATTCAATTCCGTAAAGGCGCTAAACCGATTGATTTTCGTGTCAGCACTCT
TCCGACCTTGTTCGGTGAAAAAATTGTGATGCGTATTCTGAATTCGGATGCTGCCACACTGAATATCGAACAGCTCGGTT
TCGAACCATTTCAGAAAGAATTACTGCTGGAAGCCATTCACCGGCCCTACGGTATGGTTCTAGTTACCGGCCCAACTGGT
TCCGGTAAAACCGTATCCCTCTATACCTGCTTAAATATTCTGAACACCGAAAGTGTGAACATTTCTACCGCTGAAGACCC
TGCAGAAATTAATTTACCCGGTATCAATCAGGTCAATGTAAATGATAAACAGGGACTGACATTTGCCGCAGCTCTAAAAT
CTTTCCTACGTCAGGATCCAGACATCATCATGGTGGGGGAAATCCGGGATTTAGAAACAGCCGATATTGCCATTAAAGCG
GCACAAACCGGCCATATGGTATTCTCTACCCTGCACACCAATAATGCGCCTGCTACCTTATCACGTATGCTGAATATGGG
GGTTGCTCCATTCAACATCGCAAGTTCAGTGAATCTGATTATGGCACAGCGTCTGGTTCGACGTTTATGTACCTGCAAAG
CACCAGCCGAACGGCCACCAGAGTATGCAATGAAAAAAGCTGGTTTCTCAGACGAAGATCTGGCCAAAGATTGGACATTT
TATCGGCCTGTAGGTTGTGAACGCTGTAAAGGCAAAGGTTACAAGGGTCGTGCCGGTGTTTACGAAGTAATGCCAGTAAG
TGAAGAAATGCAACGTATAATCATGAACAACGGAAATGAAGTTGATATAAGTAATCAAGCACTTAAGGAAGGACTAGTCG
ATTTACGTCATGCTGGTCTACTTAAAGTAATGGAAGGCATCACCTCGCTTGACGAAATCCTCGCCACCACCAACGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(180-448)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(37-141)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Neisseria gonorrhoeae MS11

68.75

100

0.689
  pilB Acinetobacter baylyi ADP1

50

100

0.508
  pilB Acinetobacter baumannii D1279779

52.239

95.886

0.501
  pilB Legionella pneumophila strain ERS1305867

47.979

100

0.488
  pilB Vibrio cholerae strain A1552

45.406

100

0.46
  pilB Vibrio campbellii strain DS40M4

43.838

100

0.445
  pilB Vibrio parahaemolyticus RIMD 2210633

43.44

100

0.438
  pilF Thermus thermophilus HB27

39.963

96.243

0.385
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.038

95.17

0.381

Multiple sequence alignment