Detailed information NTG1035324

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: ACA345_RS02105
• Genome accession: NZ_CP166819
• Coordinates: 454219..455367 (+)
• Length: 382 a.a.
• NCBI ID: WP_003110016.1
• Uniprot ID: A0A0H2ZL88
• Organism: Pseudomonas aeruginosa strain M6121663
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 449219..460367

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACA345_RS02080 (ACA345_02080)	-	450370..450666 (-)	297	WP_031628111.1	DUF167 domain-containing protein	-
ACA345_RS02085 (ACA345_02085)	-	450663..451256 (-)	594	WP_003084544.1	YggT family protein	-
ACA345_RS02090 (ACA345_02090)	proC	451267..452088 (-)	822	WP_003084546.1	pyrroline-5-carboxylate reductase	-
ACA345_RS02095 (ACA345_02095)	-	452100..452792 (-)	693	WP_003084549.1	YggS family pyridoxal phosphate-dependent enzyme	-
ACA345_RS02100 (ACA345_02100)	pilT	453007..454041 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
ACA345_RS02105 (ACA345_02105)	pilU	454219..455367 (+)	1149	WP_003110016.1	type IV pilus ATPase PilU	Machinery gene
ACA345_RS02110 (ACA345_02110)	czcD	455375..456274 (-)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
ACA345_RS02115 (ACA345_02115)	-	456429..456833 (+)	405	WP_003084560.1	NINE protein	-
ACA345_RS02120 (ACA345_02120)	-	457076..458449 (+)	1374	WP_004365040.1	pyridoxal-phosphate dependent enzyme	-
ACA345_RS02125 (ACA345_02125)	-	458446..459630 (+)	1185	WP_003110017.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42559.83 Da
• Isoelectric Point: 6.7792

>NTDB_id=1035324 ACA345_RS02105 WP_003110016.1 454219..455367(+) (pilU) [Pseudomonas aeruginosa strain M6121663]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSNLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=1035324 ACA345_RS02105 WP_003110016.1 454219..455367(+) (pilU) [Pseudomonas aeruginosa strain M6121663]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACTCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTACACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATAACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAACCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: A0A0H2ZL88

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.22	100	0.882
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382