Detailed information NTG1034836

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: AB9V60_RS01670
• Genome accession: NZ_CP166624
• Coordinates: 351838..352902 (+)
• Length: 354 a.a.
• NCBI ID: WP_003341394.1
• Uniprot ID: A0A0P9GH14
• Organism: Pseudomonas syringae pv. atrofaciens strain GM 2231
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 346838..357902

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
AB9V60_RS01660 (AB9V60_01660)	-	347200..348468 (+)	1269	WP_370683282.1	malic enzyme-like NAD(P)-binding protein	-
AB9V60_RS01665 (AB9V60_01665)	-	349187..351628 (-)	2442	WP_370683520.1	penicillin-binding protein 1A	-
AB9V60_RS01670 (AB9V60_01670)	pilM	351838..352902 (+)	1065	WP_003341394.1	pilus assembly protein PilM	Machinery gene
AB9V60_RS01675 (AB9V60_01675)	-	352902..353483 (+)	582	WP_003316651.1	PilN domain-containing protein	-
AB9V60_RS01680 (AB9V60_01680)	pilO	353480..354103 (+)	624	WP_003316652.1	type 4a pilus biogenesis protein PilO	-
AB9V60_RS01685 (AB9V60_01685)	pilP	354100..354627 (+)	528	WP_003316653.1	type 4a pilus biogenesis lipoprotein PilP	-
AB9V60_RS01690 (AB9V60_01690)	pilQ	354675..356780 (+)	2106	WP_003422532.1	type IV pilus secretin PilQ	Machinery gene
AB9V60_RS01695 (AB9V60_01695)	aroK	356785..357303 (+)	519	WP_003341387.1	shikimate kinase AroK	-

Sequence

Protein

• Length: 354 a.a.
• Molecular weight: 38134.68 Da
• Isoelectric Point: 4.7090

>NTDB_id=1034836 AB9V60_RS01670 WP_003341394.1 351838..352902(+) (pilM) [Pseudomonas syringae pv. atrofaciens strain GM 2231]
MFELFSKKANTLLGIDISSTSVKLLELSRSGTRYKVESYAVEPLPANAVVEKNIAELEGVGQALSRVLVKAKTSVKIVAV
AVAGSAVITKTIEMDAGLSDDDMENQLKLEADQYIPYPLEEVAIDFEVQGYSVRNPERVEVLLAACRKENVEVREAALAL
AGLTARVVDVEAYALERSFGLLAAQLGNGHDELTVAVVDIGATMTTLSVLHHGRIIYTREQLFGGRQLTDEIQRRYGLSM
EEAGLAKKQGGLPDDYVSEVLEPFKEALVQQVSRSLQFFFAAGQYNSVDHIMLAGGTASISGLEHLIQRRIGTPTMVANP
FADMALSSKVNAGALASDAPALMIACGLALRSFD

Nucleotide

• Length: 1065 bp

>NTDB_id=1034836 AB9V60_RS01670 WP_003341394.1 351838..352902(+) (pilM) [Pseudomonas syringae pv. atrofaciens strain GM 2231]
GTGTTCGAACTCTTCAGTAAGAAGGCCAACACCCTTCTAGGGATCGATATTAGCTCCACCTCGGTAAAACTCCTGGAATT
GAGTCGTTCCGGCACCCGTTACAAGGTCGAGTCTTACGCAGTAGAGCCATTGCCGGCCAACGCGGTCGTCGAAAAGAACA
TTGCCGAACTCGAAGGGGTCGGGCAGGCGTTGTCACGCGTGCTCGTCAAGGCCAAGACCAGCGTCAAGATCGTGGCGGTT
GCCGTGGCCGGTTCGGCGGTGATCACCAAGACCATCGAGATGGACGCCGGGCTTTCCGACGATGACATGGAAAACCAGCT
CAAGCTTGAAGCTGACCAGTACATCCCCTATCCGCTGGAAGAAGTCGCCATCGATTTCGAAGTGCAGGGCTATTCGGTGC
GCAACCCCGAGCGCGTCGAGGTGTTGCTGGCTGCCTGCCGCAAGGAAAACGTCGAAGTACGCGAGGCCGCGCTGGCACTG
GCCGGGCTGACCGCACGCGTCGTCGATGTCGAGGCCTACGCCCTTGAGCGTTCGTTCGGTTTGCTGGCGGCGCAACTGGG
CAATGGCCATGACGAGCTGACGGTGGCCGTGGTCGATATCGGCGCCACCATGACCACTCTGAGCGTGCTGCATCATGGCC
GGATCATCTATACCCGCGAGCAGCTGTTTGGTGGGCGCCAGTTGACTGACGAAATCCAGCGCCGCTACGGGCTCTCCATG
GAGGAAGCCGGGCTTGCCAAGAAGCAGGGCGGCTTGCCCGACGATTACGTCAGCGAGGTGCTCGAGCCCTTCAAGGAAGC
GCTGGTGCAGCAGGTTTCCCGATCCCTGCAGTTCTTCTTCGCGGCCGGTCAATACAACTCAGTGGATCACATCATGCTGG
CAGGCGGCACGGCCTCGATTTCAGGGCTTGAGCATCTGATCCAGCGGCGCATCGGCACGCCGACCATGGTCGCCAACCCG
TTTGCCGACATGGCACTGAGCTCCAAGGTCAATGCCGGCGCCCTGGCCAGTGATGCGCCGGCGCTGATGATCGCGTGCGG
CCTGGCGTTGAGGAGCTTCGACTGA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-353)

3D structure

• AlphaFold DB: A0A0P9GH14

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	55.65	100	0.557
comM	Acinetobacter nosocomialis M2	55.65	100	0.557
comM	Acinetobacter baylyi ADP1	54.237	100	0.542
pilM	Legionella pneumophila strain ERS1305867	46.761	100	0.469