Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ABK096_RS04995 Genome accession   NZ_CP166455
Coordinates   1080415..1081557 (+) Length   380 a.a.
NCBI ID   WP_005408369.1    Uniprot ID   A0A854DW57
Organism   Stenotrophomonas maltophilia strain a6     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1075415..1086557
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABK096_RS04970 (ABK096_04970) - 1075919..1076521 (-) 603 WP_049397188.1 short chain dehydrogenase -
  ABK096_RS04975 (ABK096_04975) - 1076620..1077495 (+) 876 WP_060381038.1 LysR family transcriptional regulator -
  ABK096_RS04980 (ABK096_04980) proC 1077672..1078493 (-) 822 WP_012479334.1 pyrroline-5-carboxylate reductase -
  ABK096_RS04985 (ABK096_04985) - 1078512..1079189 (-) 678 WP_012479335.1 YggS family pyridoxal phosphate-dependent enzyme -
  ABK096_RS04990 (ABK096_04990) pilT 1079277..1080314 (+) 1038 WP_004147099.1 type IV pilus twitching motility protein PilT Machinery gene
  ABK096_RS04995 (ABK096_04995) pilU 1080415..1081557 (+) 1143 WP_005408369.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ABK096_RS05000 (ABK096_05000) - 1081765..1082748 (+) 984 WP_049397194.1 aldo/keto reductase -
  ABK096_RS05005 (ABK096_05005) - 1082796..1083695 (-) 900 WP_005412489.1 LysR family transcriptional regulator -
  ABK096_RS05010 (ABK096_05010) - 1083789..1083998 (+) 210 WP_005412490.1 DUF4287 domain-containing protein -
  ABK096_RS05015 (ABK096_05015) - 1084109..1084816 (+) 708 WP_014036228.1 YitT family protein -
  ABK096_RS05020 (ABK096_05020) - 1084900..1085445 (-) 546 WP_049395504.1 DNA-3-methyladenine glycosylase I -

Sequence

Protein


Download         Length: 380 a.a.        Molecular weight: 42355.69 Da        Isoelectric Point: 6.7683

>NTDB_id=1034566 ABK096_RS04995 WP_005408369.1 1080415..1081557(+) (pilU) [Stenotrophomonas maltophilia strain a6]
MNTTATTIDFTSFLKLMAHQRASDLFITAGMPPAMKVNGKISPITQTPLTPQQSRDLVLNVMTPAQREEFEKTHECNFAI
GLSGVGRFRVSCFYQRNQVGMVLRRIETRIPTVEELSLPPIIKTLAMTKRGIILFVGATGTGKSTSLAAMIGYRNQNSTG
HIITIEDPIEFVHKHEGCIITQREVGIDTDSWEAALKNTLRQAPDVIMIGEVRTREGMDHAIAFAETGHLVLCTLHANNA
NQAMDRIVNFFPEDRRNQLLMDLSLNLKGVVAQQLVPSPDGRSRKVAMEILLGTPLVQDYIRDGEIHKLKEVMKDSVQLG
MKTFDQSLFELYQAGEISYEDALRYADSQNEVRLRIKLSQGGDARTLSQGLDGVEISEIR

Nucleotide


Download         Length: 1143 bp        

>NTDB_id=1034566 ABK096_RS04995 WP_005408369.1 1080415..1081557(+) (pilU) [Stenotrophomonas maltophilia strain a6]
GTGAACACCACCGCGACCACCATCGATTTCACTTCGTTCCTCAAGCTGATGGCGCACCAGCGCGCCTCGGACCTGTTCAT
CACTGCGGGCATGCCGCCGGCGATGAAGGTCAACGGCAAGATCTCGCCGATCACGCAGACCCCGCTCACGCCGCAGCAGA
GCCGCGACCTGGTCCTCAACGTGATGACCCCGGCGCAGCGCGAGGAATTCGAGAAGACCCACGAATGCAACTTCGCCATC
GGCCTGTCCGGTGTCGGCCGCTTCCGCGTCAGCTGCTTCTACCAGCGCAACCAGGTCGGCATGGTGCTGCGTCGCATCGA
GACGCGCATCCCGACGGTGGAAGAACTGAGCTTGCCGCCGATTATCAAGACGCTGGCGATGACCAAGCGCGGCATCATCC
TGTTCGTCGGTGCCACCGGTACCGGTAAATCGACCTCGCTGGCAGCGATGATCGGTTACCGCAACCAGAACTCGACCGGC
CACATCATCACCATCGAAGATCCGATCGAGTTCGTGCACAAGCACGAGGGCTGCATCATCACCCAGCGCGAAGTCGGCAT
CGATACCGACAGCTGGGAAGCCGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGTGAGGTGCGTA
CCCGCGAAGGCATGGACCACGCCATCGCGTTCGCCGAAACCGGCCACCTGGTGCTGTGCACCCTGCATGCCAACAACGCC
AACCAGGCGATGGACCGCATCGTCAATTTCTTCCCGGAAGACCGCCGCAACCAGCTGCTGATGGACCTGTCGCTGAATCT
CAAGGGCGTGGTCGCGCAGCAGCTGGTGCCGTCGCCCGATGGCCGCTCGCGCAAGGTGGCGATGGAGATCCTGCTGGGCA
CGCCGCTGGTGCAGGACTACATCCGCGACGGCGAGATCCACAAGCTGAAGGAAGTGATGAAGGACTCGGTCCAGCTGGGC
ATGAAGACCTTCGACCAGAGCCTGTTCGAGCTGTACCAGGCCGGCGAGATCAGCTACGAGGACGCGCTGCGTTACGCCGA
TTCGCAGAACGAAGTGCGCCTGCGCATCAAGCTCAGCCAGGGCGGCGACGCACGCACGCTGTCACAGGGCCTGGATGGCG
TGGAGATCTCCGAGATCCGGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(122-277)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A854DW57

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

67.847

96.579

0.655
  pilU Acinetobacter baylyi ADP1

63.585

93.947

0.597
  pilU Vibrio cholerae strain A1552

53.371

93.684

0.5
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.099

93.421

0.403

Multiple sequence alignment