Detailed information NTG1032590

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: AB8E34_RS09125
• Genome accession: NZ_CP165964
• Coordinates: 2044372..2045436 (-)
• Length: 354 a.a.
• NCBI ID: WP_318644764.1
• Uniprot ID: -
• Organism: Pseudomonas benzenivorans strain jeju3-2
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2039372..2050436

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
AB8E34_RS09100 (AB8E34_09050)	aroK	2039986..2040504 (-)	519	WP_318644768.1	shikimate kinase AroK	-
AB8E34_RS09105 (AB8E34_09055)	pilQ	2040509..2042604 (-)	2096	Protein_1773	type IV pilus secretin PilQ	-
AB8E34_RS09110 (AB8E34_09060)	pilP	2042659..2043183 (-)	525	WP_318644767.1	type 4a pilus biogenesis lipoprotein PilP	-
AB8E34_RS09115 (AB8E34_09065)	pilO	2043183..2043803 (-)	621	WP_369960173.1	type 4a pilus biogenesis protein PilO	-
AB8E34_RS09120 (AB8E34_09070)	pilN	2043800..2044372 (-)	573	WP_369960175.1	type 4a pilus biogenesis protein PilN	-
AB8E34_RS09125 (AB8E34_09075)	pilM	2044372..2045436 (-)	1065	WP_318644764.1	pilus assembly protein PilM	Machinery gene
AB8E34_RS09130 (AB8E34_09080)	-	2045621..2048041 (+)	2421	WP_369960177.1	penicillin-binding protein 1A	-
AB8E34_RS09135 (AB8E34_09085)	-	2048135..2049403 (-)	1269	WP_318644762.1	malic enzyme-like NAD(P)-binding protein	-

Sequence

Protein

• Length: 354 a.a.
• Molecular weight: 37860.11 Da
• Isoelectric Point: 4.3692

>NTDB_id=1032590 AB8E34_RS09125 WP_318644764.1 2044372..2045436(-) (pilM) [Pseudomonas benzenivorans strain jeju3-2]
MLGLFNKNANTLLGIDISSTSVKLLELSRSGSRYKVEAYAVEPLPPNAVVEKNIAELEGVGQALTRVLSKARSGVKSVAV
AVAGSAVITKTIEMEAGLSDDDLENQLKIEADQYIPYPLEEVAIDFEVQGPSPRNPDRVEVLLAACRKENVEVREAALAL
SGLTAKVVDVEAYALERAYGLLAEQLGGGQEVLTVAVVDIGATMTTLSVLHDGRTIYTREQLFGGKQLTEEIQRRYGLSV
EEAGLAKKQGGLPDDYDSEVLQPFKEAVVQQVSRSLQFFFAAGQFNDVDYILLAGGTASIPDLDRLIQQKIGTQTLVANP
FADMALGSRVNAGALASDAPSLMIACGLALRSFD

Nucleotide

• Length: 1065 bp

>NTDB_id=1032590 AB8E34_RS09125 WP_318644764.1 2044372..2045436(-) (pilM) [Pseudomonas benzenivorans strain jeju3-2]
GTGCTAGGGCTCTTCAATAAGAATGCGAATACGCTGCTGGGGATCGATATCAGTTCGACCTCGGTCAAGCTCCTCGAGTT
GAGTCGCTCCGGAAGCCGCTACAAGGTAGAGGCTTACGCTGTCGAGCCACTCCCCCCAAATGCTGTGGTCGAGAAGAACA
TCGCCGAGTTGGAAGGCGTTGGTCAGGCGCTTACGCGCGTGTTGAGCAAGGCCAGGAGCGGCGTGAAATCGGTCGCCGTG
GCGGTGGCCGGCTCGGCAGTGATCACCAAGACCATCGAGATGGAGGCCGGGCTTTCCGACGATGACCTCGAGAATCAGCT
GAAGATCGAGGCTGATCAGTACATCCCCTATCCCCTCGAAGAAGTGGCGATCGACTTCGAAGTGCAAGGGCCTTCGCCGC
GTAACCCCGACCGTGTCGAGGTGCTCCTCGCAGCCTGTCGCAAGGAGAACGTCGAGGTTCGAGAGGCCGCGCTGGCCCTG
TCCGGCTTGACCGCCAAGGTGGTCGACGTCGAAGCCTATGCCCTGGAGCGCGCCTACGGCCTGCTTGCGGAGCAACTGGG
CGGCGGGCAAGAGGTGCTGACAGTGGCGGTGGTCGATATCGGTGCGACCATGACGACCCTGAGCGTCCTGCATGATGGGC
GGACCATCTATACGCGCGAGCAACTGTTCGGGGGCAAGCAGCTGACTGAGGAGATCCAGCGGCGTTACGGGCTGTCGGTT
GAGGAAGCTGGGCTCGCCAAGAAGCAGGGAGGCCTTCCTGACGACTATGACAGTGAGGTGTTGCAGCCGTTCAAGGAGGC
TGTCGTACAGCAGGTCTCGCGCTCCTTGCAGTTCTTCTTCGCGGCGGGCCAGTTCAATGATGTCGACTACATCCTCCTGG
CCGGCGGGACGGCCTCCATTCCGGATCTGGACCGGCTGATACAGCAGAAGATCGGTACTCAAACGCTGGTGGCCAACCCG
TTCGCGGATATGGCTCTGGGCAGCAGAGTCAATGCCGGGGCATTGGCCAGCGACGCTCCGTCATTGATGATTGCCTGTGG
GCTGGCGCTGAGGAGTTTTGACTAA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-353)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	56.497	100	0.565
comM	Acinetobacter nosocomialis M2	56.497	100	0.565
comM	Acinetobacter baylyi ADP1	54.237	100	0.542
pilM	Legionella pneumophila strain ERS1305867	45.48	100	0.455