Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   AB8E34_RS01420 Genome accession   NZ_CP165964
Coordinates   337591..338802 (-) Length   403 a.a.
NCBI ID   WP_369959365.1    Uniprot ID   -
Organism   Pseudomonas benzenivorans strain jeju3-2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
IS/Tn 336680..337081 337591..338802 flank 510


Gene organization within MGE regions


Location: 336680..338802
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AB8E34_RS01415 (AB8E34_01415) - 337189..337190 (-) 2 WP_369959364.1 IS5 family transposase -
  AB8E34_RS01420 (AB8E34_01420) pilU 337591..338802 (-) 1212 WP_369959365.1 PilT/PilU family type 4a pilus ATPase Machinery gene

Sequence

Protein


Download         Length: 403 a.a.        Molecular weight: 44449.72 Da        Isoelectric Point: 6.0824

>NTDB_id=1032587 AB8E34_RS01420 WP_369959365.1 337591..338802(-) (pilU) [Pseudomonas benzenivorans strain jeju3-2]
MDLHSLLKILASQDGSDLYLSTGAPPCAKFNGVLKALGQEPLKSGDVAQVAESIMDSAQREEFERELEMNLAISLAGIGR
FRINIFKQRNEVSIVARNIKMEIPKFEDLKLPEVLLKTVMEKRGLVLFVGGTGSGKSTSLAALIDYRNRNSGGHIITIED
PVEYVHRHKKSIINQREVGVDTRSFHAALKNTLRQAPDVILIGEIRDRETMEHALAFADTGHLAISTLHANNANQALDRI
INFFPEERRPQLLNDLGNNLKAFVSQRLVKTADGKRRAAVEVMLGTPTVRDLIKRNEFSELKEIMEKSKNLGMQTFDQAL
IDLVHEGAIDEEEAVKNADSANNVRLKLKLYRDNPATPSPAPVAEKAAATPAAASEKGDWGLELKLEAIEEDSPPEDPGR
TGI

Nucleotide


Download         Length: 1212 bp        

>NTDB_id=1032587 AB8E34_RS01420 WP_369959365.1 337591..338802(-) (pilU) [Pseudomonas benzenivorans strain jeju3-2]
ATGGACCTTCATTCGCTGCTGAAAATCCTGGCCAGCCAGGACGGTTCCGATCTCTACCTGTCCACCGGTGCGCCACCCTG
TGCGAAGTTCAACGGCGTGCTCAAGGCCCTCGGTCAGGAGCCGTTGAAGTCCGGCGATGTGGCGCAGGTGGCCGAGTCGA
TCATGGACAGCGCGCAGCGCGAGGAATTCGAGCGCGAACTGGAGATGAACCTGGCGATTTCCCTGGCCGGGATCGGCCGC
TTCCGCATCAATATCTTCAAGCAGCGCAACGAAGTCTCGATCGTCGCGCGCAACATCAAGATGGAGATCCCCAAGTTCGA
GGACCTCAAGCTGCCCGAGGTGTTGCTCAAGACCGTGATGGAGAAGCGCGGCCTGGTACTGTTCGTCGGCGGCACCGGTT
CGGGCAAGTCCACCTCCCTGGCGGCGTTGATCGACTACCGCAACCGCAACAGCGGCGGTCATATCATCACCATCGAGGAT
CCGGTGGAGTACGTGCACCGGCACAAGAAGTCGATCATCAACCAGCGCGAGGTCGGGGTCGACACCCGCAGCTTCCACGC
CGCGCTGAAGAACACCCTGCGCCAGGCCCCGGACGTGATCCTGATCGGCGAGATCCGCGACCGCGAGACCATGGAGCACG
CCCTGGCCTTCGCCGACACCGGCCACCTGGCCATCTCCACCCTGCACGCCAACAACGCCAACCAGGCGCTGGACCGCATC
ATCAACTTCTTCCCCGAGGAGCGCCGCCCGCAACTGCTCAACGACCTGGGCAACAACCTCAAGGCCTTCGTCTCCCAGCG
CCTGGTCAAGACCGCCGATGGCAAGCGCCGTGCCGCGGTGGAGGTGATGCTGGGCACGCCGACGGTGCGCGACCTGATCA
AGCGCAACGAATTCTCCGAGCTCAAGGAGATCATGGAGAAGTCGAAGAACCTCGGCATGCAGACCTTCGACCAGGCGCTG
ATCGACCTGGTCCACGAGGGCGCCATCGACGAGGAAGAGGCGGTGAAGAACGCCGATTCGGCGAACAACGTGCGCCTCAA
GCTCAAGCTGTACCGGGACAACCCGGCCACGCCGAGCCCGGCCCCCGTGGCCGAGAAGGCCGCCGCGACGCCTGCCGCGG
CGAGCGAGAAGGGCGACTGGGGGCTGGAGCTGAAACTGGAGGCGATCGAGGAAGATTCGCCGCCCGAGGACCCGGGGCGG
ACGGGGATCTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(49-248)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

56.695

87.097

0.494
  pilU Vibrio cholerae strain A1552

52.125

87.593

0.457
  pilU Acinetobacter baylyi ADP1

52.273

87.345

0.457
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.051

97.27

0.39

Multiple sequence alignment