Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA/cflA   Type   Machinery gene
Locus tag   AB6M99_RS08005 Genome accession   NZ_CP163513
Coordinates   1665632..1666924 (-) Length   430 a.a.
NCBI ID   WP_121835775.1    Uniprot ID   A0A3L9DUS7
Organism   Streptococcus hillyeri strain S23-3001-2     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 1645138..1665635 1665632..1666924 flank -3


Gene organization within MGE regions


Location: 1645138..1666924
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AB6M99_RS07865 (AB6M99_07865) - 1645138..1645875 (-) 738 WP_121835751.1 helix-turn-helix domain-containing protein -
  AB6M99_RS07870 (AB6M99_07870) - 1646060..1646245 (+) 186 WP_121835752.1 helix-turn-helix transcriptional regulator -
  AB6M99_RS07875 (AB6M99_07875) - 1646335..1646568 (+) 234 WP_121835753.1 hypothetical protein -
  AB6M99_RS07880 (AB6M99_07880) - 1646737..1647045 (+) 309 WP_121835754.1 hypothetical protein -
  AB6M99_RS07885 (AB6M99_07885) - 1647026..1647232 (+) 207 WP_121835755.1 hypothetical protein -
  AB6M99_RS07890 (AB6M99_07890) - 1647219..1647698 (+) 480 WP_245962708.1 hypothetical protein -
  AB6M99_RS07895 (AB6M99_07895) - 1647691..1648098 (+) 408 WP_121835756.1 hypothetical protein -
  AB6M99_RS07900 (AB6M99_07900) - 1648227..1648490 (+) 264 WP_121835757.1 hypothetical protein -
  AB6M99_RS07905 (AB6M99_07905) - 1648920..1649150 (+) 231 WP_121835758.1 hypothetical protein -
  AB6M99_RS07910 (AB6M99_07910) - 1649135..1649329 (+) 195 WP_121835759.1 hypothetical protein -
  AB6M99_RS07915 (AB6M99_07915) - 1649343..1649681 (+) 339 WP_121835760.1 HTH domain-containing protein -
  AB6M99_RS07920 (AB6M99_07920) - 1649890..1650228 (+) 339 WP_142925595.1 hypothetical protein -
  AB6M99_RS07925 (AB6M99_07925) - 1650185..1651711 (+) 1527 WP_121835762.1 phage/plasmid primase, P4 family -
  AB6M99_RS07930 (AB6M99_07930) - 1652232..1652773 (+) 542 Protein_1547 site-specific integrase -
  AB6M99_RS07935 (AB6M99_07935) - 1653563..1654231 (+) 669 WP_121835765.1 Fic family protein -
  AB6M99_RS07940 (AB6M99_07940) - 1654853..1655005 (+) 153 WP_183121669.1 hypothetical protein -
  AB6M99_RS07945 (AB6M99_07945) - 1655024..1656526 (+) 1503 WP_369351206.1 ATP-binding cassette domain-containing protein -
  AB6M99_RS07950 (AB6M99_07950) - 1656542..1656712 (-) 171 WP_121835767.1 hypothetical protein -
  AB6M99_RS07955 (AB6M99_07955) - 1656729..1657283 (-) 555 WP_121835768.1 GNAT family N-acetyltransferase -
  AB6M99_RS07960 (AB6M99_07960) obgE 1657478..1658791 (-) 1314 WP_121835769.1 GTPase ObgE -
  AB6M99_RS07965 (AB6M99_07965) - 1659058..1659186 (-) 129 WP_083959937.1 DUF4044 domain-containing protein -
  AB6M99_RS07970 (AB6M99_07970) - 1659329..1660579 (+) 1251 WP_369350515.1 aminopeptidase -
  AB6M99_RS07975 (AB6M99_07975) asnA 1660692..1661684 (-) 993 WP_121835771.1 aspartate--ammonia ligase -
  AB6M99_RS07980 (AB6M99_07980) - 1661765..1662829 (-) 1065 WP_121835772.1 MmcQ/YjbR family DNA-binding protein -
  AB6M99_RS07990 (AB6M99_07990) rplS 1663228..1663575 (-) 348 WP_027971744.1 50S ribosomal protein L19 -
  AB6M99_RS07995 (AB6M99_07995) raiA 1664338..1664886 (-) 549 WP_121835773.1 ribosome-associated translation inhibitor RaiA -
  AB6M99_RS08000 (AB6M99_08000) comFC/cflB 1664973..1665635 (-) 663 WP_121835774.1 ComF family protein Machinery gene
  AB6M99_RS08005 (AB6M99_08005) comFA/cflA 1665632..1666924 (-) 1293 WP_121835775.1 DEAD/DEAH box helicase Machinery gene

Sequence

Protein


Download         Length: 430 a.a.        Molecular weight: 48745.72 Da        Isoelectric Point: 8.6825

>NTDB_id=1030303 AB6M99_RS08005 WP_121835775.1 1665632..1666924(-) (comFA/cflA) [Streptococcus hillyeri strain S23-3001-2]
MEDFYGRLFVAHQLSDEEKSRAKQLPAMLEQKGKLCCQRCGSHILTDWLLPAGDYYCRECLMLGRNRQSQPLYYFPAQPF
SKEKYLIWQGTLTSYQQEISDGMKTAVANKENILIHAVTGAGKTEMIYETVASVLEQGGQVCLASPRIDVCLELHKRLSR
DFSCPIALLHGESEPYTRAPLVIATTHQLLKFYQAFDLLIIDEVDAFPFVDNAMLYHGLNQAVKIDGVKVFLTATSTDAL
DKQVKQGKLKKLDLARRFHANPLVVPKMKWLSGLLFSLQKDKLPSKLLKQVASQRETGYPLLIFFPHIEIGQRFTEILTK
TFPQETIGFVASTTENRLELVQQFRDKKLTVLVSTTILERGVTFPCVDVFVLWSNHKLYTRSSLVQIGGRVGRAMERPTG
ELIFFHDGMTIEMARAIAEIRLMNQKGGFA

Nucleotide


Download         Length: 1293 bp        

>NTDB_id=1030303 AB6M99_RS08005 WP_121835775.1 1665632..1666924(-) (comFA/cflA) [Streptococcus hillyeri strain S23-3001-2]
ATGGAAGATTTTTACGGACGTTTATTTGTAGCGCATCAACTTTCCGATGAGGAAAAAAGCAGAGCTAAACAGTTACCAGC
CATGCTAGAGCAAAAAGGGAAACTCTGTTGTCAGCGATGTGGCAGTCACATATTAACAGACTGGCTGTTGCCAGCTGGTG
ACTATTATTGCAGGGAGTGTCTTATGTTAGGGAGAAATCGACAAAGCCAGCCTCTCTATTATTTCCCAGCGCAGCCTTTT
TCTAAAGAAAAGTATCTGATCTGGCAAGGAACTCTGACGTCGTACCAACAGGAAATCTCAGATGGCATGAAAACTGCTGT
GGCTAATAAGGAAAATATCCTTATTCATGCGGTGACAGGGGCTGGAAAGACCGAAATGATTTATGAGACAGTGGCAAGTG
TTTTGGAGCAAGGTGGACAGGTTTGTCTAGCTAGTCCACGGATTGATGTCTGTCTAGAACTTCATAAACGCCTTAGCAGA
GATTTTTCTTGTCCCATAGCGCTCTTGCATGGTGAGAGTGAGCCTTATACTAGAGCGCCCTTGGTTATTGCAACAACCCA
TCAGTTGCTTAAATTTTACCAAGCCTTTGATTTACTGATTATAGATGAGGTGGACGCTTTTCCTTTCGTTGATAATGCCA
TGCTTTATCATGGTCTTAACCAAGCGGTTAAAATTGATGGCGTCAAAGTTTTTTTGACAGCTACCTCAACGGATGCATTG
GATAAACAAGTCAAACAAGGAAAGCTTAAAAAGTTAGATTTAGCCAGACGTTTCCACGCCAATCCTCTCGTGGTTCCCAA
AATGAAGTGGCTGAGTGGACTGTTATTTAGTCTTCAAAAAGACAAACTCCCTTCAAAATTGTTGAAACAGGTAGCCTCAC
AAAGAGAAACGGGTTATCCACTTTTGATTTTCTTTCCTCACATTGAGATAGGACAAAGGTTTACTGAAATCTTAACGAAA
ACATTTCCACAGGAAACTATTGGGTTTGTTGCCTCAACAACAGAAAATCGTTTAGAATTAGTTCAGCAGTTTAGAGATAA
AAAGCTGACTGTTTTGGTATCAACCACTATCTTAGAGAGGGGAGTTACCTTTCCCTGTGTAGATGTTTTTGTACTTTGGA
GCAACCATAAACTCTATACGCGGTCCTCTCTTGTGCAGATTGGTGGCCGTGTCGGAAGAGCAATGGAGAGACCAACAGGA
GAATTGATTTTCTTTCACGATGGCATGACTATAGAGATGGCAAGAGCCATTGCTGAGATTCGCCTGATGAATCAAAAAGG
AGGCTTTGCATGA

Domains

Predicted by InterproScan.

Helicase conserved C-terminal domain (Helicase_C)

(286-394)

Type III restriction enzyme, res subunit (ResIII)

(91-203)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A3L9DUS7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA/cflA Streptococcus mitis NCTC 12261

61.593

99.302

0.612
  comFA/cflA Streptococcus pneumoniae Rx1

61.593

99.302

0.612
  comFA/cflA Streptococcus pneumoniae D39

61.593

99.302

0.612
  comFA/cflA Streptococcus pneumoniae R6

61.593

99.302

0.612
  comFA/cflA Streptococcus pneumoniae TIGR4

61.358

99.302

0.609
  comFA/cflA Streptococcus mitis SK321

61.358

99.302

0.609
  comFA Lactococcus lactis subsp. cremoris KW2

51.256

92.558

0.474
  comFA Latilactobacillus sakei subsp. sakei 23K

36.891

100

0.37
  comFA Bacillus subtilis subsp. subtilis str. 168

37.805

95.349

0.36

Multiple sequence alignment