Detailed information NTG1029478

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: AB4670_RS14375
• Genome accession: NZ_CP163382
• Coordinates: 3005381..3006499 (+)
• Length: 372 a.a.
• NCBI ID: WP_000347036.1
• Uniprot ID: A0A009PAD6
• Organism: Acinetobacter baumannii strain Y03
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3000381..3011499

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
AB4670_RS14365 (AB4670_14355)	-	3003505..3004197 (-)	693	WP_199989547.1	YggS family pyridoxal phosphate-dependent enzyme	-
AB4670_RS14370 (AB4670_14360)	pilT	3004324..3005361 (+)	1038	WP_000355489.1	type IV pilus twitching motility protein PilT	Machinery gene
AB4670_RS14375 (AB4670_14365)	pilU	3005381..3006499 (+)	1119	WP_000347036.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
AB4670_RS14380 (AB4670_14370)	fur	3006569..3007006 (-)	438	WP_001122847.1	ferric iron uptake transcriptional regulator	-
AB4670_RS14385 (AB4670_14375)	-	3007118..3007516 (+)	399	WP_001170994.1	outer membrane protein assembly factor BamE	-
AB4670_RS14390 (AB4670_14380)	-	3007571..3007894 (-)	324	WP_114259159.1	RnfH family protein	-
AB4670_RS14395 (AB4670_14385)	-	3007894..3008961 (-)	1068	WP_000092403.1	hypothetical protein	-
AB4670_RS14400 (AB4670_14390)	-	3009114..3009569 (-)	456	WP_000782976.1	bacteriohemerythrin	-
AB4670_RS14405 (AB4670_14395)	-	3009823..3010671 (-)	849	WP_000336551.1	class II glutamine amidotransferase	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41905.11 Da
• Isoelectric Point: 6.6944

>NTDB_id=1029478 AB4670_RS14375 WP_000347036.1 3005381..3006499(+) (pilU) [Acinetobacter baumannii strain Y03]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRKEFAETRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLADEGPDQLSDGKQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=1029478 AB4670_RS14375 WP_000347036.1 3005381..3006499(+) (pilU) [Acinetobacter baumannii strain Y03]
ATGGATTTTAATGACCTGCTCAACCTCATGGTTGAAAAAAAATCATCCGATCTCTTTATTACAGATGGCGTTGCGCCCTC
TATGAAGATTAACGGGCAAATTGTTCCAATTTCAAAAAACAGTCTTTCTGGGGAAGTGATTGGTCAACTGTTACATTCCA
TCATGAGTGAAAAACAACGCAAAGAATTTGCAGAAACTCGTGAATGTAACTTTGCCATTATGAACCGTGAAAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAGCGCGACATGCCAGGCATGGTACTACGTCGTATTGAAACCAAGATTCCTTCAAT
TGATGACTTGCAGTTACCGCCTGTACTTAAAGATTTATCGATGACCAAACGCGGCATTATTATTTTCGTAGGCGCGACAG
GTACAGGTAAATCCACTTCATTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAGGGCCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCACGAACATGCAGGCTGCATCATTACTCAGCGTGAAGTCGGGATCGATACCGACTCATTTGA
AATTGCCTTGAAAAATACACTACGACAAGCGCCGGATGTGATCTTAATTGGTGAGATCCGTTCTCGTGAAGTCATGGACT
ACGCGATTGGCTTTGCTGAAACAGGCCATCTTGTATTAGCTACCATGCACGCTAACAACGCCAACCAAGCGCTCGACCGT
ATTATTCACTTTTTTGAAAGTGACCGTCATAGTCAGCTTTACATGGACTTGTCACTTAACTTAAAGGCAATGATTGCGCA
GCAGCTCATTCCAACACCAGATGGTAATTCACGCCGTGCAGCAATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAAGATTTGATGAAACGCTCACGTGAACTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATATAAAGCTGGTCAAATTACCTACAAAGATGCTCTTAAACATGCTGACTCACCGAACGATTTACG
TTTAACCATTAAACTTGCTGATGAAGGTCCCGATCAATTATCAGACGGGAAACAGCATTTAACTTTTGACCGCCAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (31-270)

3D structure

• AlphaFold DB: A0A009PAD6

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.926	100	0.772
pilU	Pseudomonas stutzeri DSM 10701	69.293	98.925	0.685
pilU	Vibrio cholerae strain A1552	53.243	99.462	0.53
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.197	93.011	0.392