Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   AB4P93_RS23275 Genome accession   NZ_CP162596
Coordinates   4896465..4897529 (+) Length   354 a.a.
NCBI ID   WP_210702919.1    Uniprot ID   -
Organism   Pseudomonas sp. B26140     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4891465..4902529
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AB4P93_RS23265 (AB4P93_23215) - 4892195..4893463 (+) 1269 WP_042557260.1 malic enzyme-like NAD(P)-binding protein -
  AB4P93_RS23270 (AB4P93_23220) - 4893801..4896248 (-) 2448 WP_210702918.1 penicillin-binding protein 1A -
  AB4P93_RS23275 (AB4P93_23225) pilM 4896465..4897529 (+) 1065 WP_210702919.1 pilus assembly protein PilM Machinery gene
  AB4P93_RS23280 (AB4P93_23230) - 4897529..4898095 (+) 567 WP_150593940.1 PilN domain-containing protein -
  AB4P93_RS23285 (AB4P93_23235) pilO 4898092..4898715 (+) 624 WP_210702920.1 type 4a pilus biogenesis protein PilO -
  AB4P93_RS23290 (AB4P93_23240) - 4898712..4899239 (+) 528 WP_210702921.1 pilus assembly protein PilP -
  AB4P93_RS23295 (AB4P93_23245) pilQ 4899253..4901328 (+) 2076 WP_395593313.1 type IV pilus secretin PilQ Machinery gene
  AB4P93_RS23300 (AB4P93_23250) aroK 4901333..4901851 (+) 519 WP_042557267.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 38091.60 Da        Isoelectric Point: 4.7417

>NTDB_id=1027352 AB4P93_RS23275 WP_210702919.1 4896465..4897529(+) (pilM) [Pseudomonas sp. B26140]
MLGLFNKKTNALLGIDISSTSVKLLELSRQGDRYRVEAYAVEPLPPNAVVEKNIAELEGVGQALTRVLGKARTGLRGVAV
AVAGSAVITKIIEMDAGLSDDELENQLKIEADQYIPYPLDEVAIDFEVQGVSPRNPERVNVLLAACRKENVEVREAALAL
AGLTARVVDVEAYALERSFGLLATQLAASQERLTVAVVDIGATMTTLSVLHNGKIIYTREQLFGGRQLTEEIQRRYGLTI
EQAGLAKKQGGLPDDYVSEVLQPFREALVQQVSRSLQFFFASGQYNAVDHILLAGGTASVPGLDRLIEQRLDTPTQVANP
FSNMALSSKVNAGALASDAPALMIACGLALRSFD

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=1027352 AB4P93_RS23275 WP_210702919.1 4896465..4897529(+) (pilM) [Pseudomonas sp. B26140]
GTGCTGGGACTCTTCAATAAAAAGACCAATGCGTTACTGGGGATCGACATCAGCTCCACGTCGGTGAAGCTGCTGGAACT
TAGTCGTCAGGGTGATCGCTACCGGGTCGAGGCTTATGCGGTGGAGCCGCTGCCACCGAACGCCGTGGTCGAAAAAAACA
TCGCCGAGCTCGAGGGCGTGGGCCAGGCCCTGACCCGGGTGCTGGGCAAGGCACGCACCGGGCTGAGAGGCGTGGCGGTG
GCAGTGGCCGGGTCAGCGGTGATTACCAAGATCATCGAGATGGACGCCGGACTTTCCGACGACGAACTGGAAAACCAGCT
CAAGATTGAAGCCGACCAATACATTCCCTATCCGCTGGATGAAGTCGCCATCGACTTCGAGGTTCAGGGTGTGTCACCGC
GCAACCCCGAGCGGGTCAACGTGTTGCTGGCCGCCTGTCGCAAGGAAAACGTCGAAGTCCGTGAAGCGGCACTGGCGCTG
GCCGGGTTGACCGCGCGTGTGGTGGACGTCGAAGCGTACGCGCTGGAGCGTTCGTTCGGCTTGCTCGCGACGCAGCTGGC
GGCTTCTCAAGAGCGCTTGACCGTGGCGGTGGTCGACATCGGCGCCACCATGACCACCCTCAGCGTCCTGCACAACGGCA
AGATCATCTACACCCGCGAGCAGTTGTTCGGCGGCCGGCAATTGACCGAGGAGATCCAGCGTCGCTATGGCCTGACCATC
GAGCAGGCGGGGCTGGCAAAGAAGCAGGGCGGTCTGCCCGACGATTACGTCAGCGAGGTGTTGCAGCCGTTTCGCGAGGC
GCTTGTACAACAGGTATCGCGTTCGTTGCAGTTTTTCTTCGCTTCCGGCCAGTACAACGCGGTCGATCATATTCTGCTCG
CTGGCGGCACGGCGTCGGTGCCGGGGCTGGACCGGCTGATCGAACAGCGCCTGGATACTCCGACCCAGGTGGCCAATCCG
TTTTCCAATATGGCCCTGAGCAGCAAGGTCAACGCCGGGGCGCTGGCCAGTGACGCGCCGGCCTTGATGATCGCCTGCGG
GCTCGCGCTCAGGAGTTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

56.497

100

0.565
  comM Acinetobacter nosocomialis M2

56.497

100

0.565
  comM Acinetobacter baylyi ADP1

55.367

100

0.554
  pilM Legionella pneumophila strain ERS1305867

46.893

100

0.469

Multiple sequence alignment