Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   AB4P95_RS19525 Genome accession   NZ_CP162583
Coordinates   4243284..4244348 (-) Length   354 a.a.
NCBI ID   WP_042557262.1    Uniprot ID   A0A9X4C1P0
Organism   Pseudomonas sp. A1437     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4238284..4249348
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AB4P95_RS19500 (AB4P95_19470) aroK 4238962..4239480 (-) 519 WP_042557267.1 shikimate kinase AroK -
  AB4P95_RS19505 (AB4P95_19475) pilQ 4239485..4241560 (-) 2076 WP_395599503.1 type IV pilus secretin PilQ Machinery gene
  AB4P95_RS19510 (AB4P95_19480) - 4241574..4242101 (-) 528 WP_395599504.1 pilus assembly protein PilP -
  AB4P95_RS19515 (AB4P95_19485) pilO 4242098..4242721 (-) 624 WP_042557264.1 type 4a pilus biogenesis protein PilO -
  AB4P95_RS19520 (AB4P95_19490) - 4242718..4243284 (-) 567 WP_042557263.1 PilN domain-containing protein -
  AB4P95_RS19525 (AB4P95_19495) pilM 4243284..4244348 (-) 1065 WP_042557262.1 pilus assembly protein PilM Machinery gene
  AB4P95_RS19530 (AB4P95_19500) - 4244565..4247012 (+) 2448 WP_395599505.1 penicillin-binding protein 1A -
  AB4P95_RS19535 (AB4P95_19505) - 4247360..4248628 (-) 1269 WP_042557260.1 malic enzyme-like NAD(P)-binding protein -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 38107.60 Da        Isoelectric Point: 4.7417

>NTDB_id=1027210 AB4P95_RS19525 WP_042557262.1 4243284..4244348(-) (pilM) [Pseudomonas sp. A1437]
MLGLFNKKTNALLGIDISSTSVKLLELSRQGDRYRVEAYAVEPLPPNAVVEKNIAELEGVGQALTRVLGKARTGLRSVAV
AVAGSAVITKIIEMDAGLSDDELENQLKIEADQYIPYPLDEVAIDFEVQGVSPRNPERVNVLLAACRKENVEVREAALAL
AGLTARVVDVEAYALERSFGLLATQLAASQERLTVAVVDIGATMTTLSVLHNGKIIYTREQLFGGRQLTEEIQRRYGLTV
EQAGLAKKQGGLPDDYVSEVLQPFREALVQQVSRSLQFFFASGQYNAVDHILLAGGTASVPGLDRLIEQRLDTPTQVANP
FSNMALSSKVNAGALASDAPALMIACGLALRSFD

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=1027210 AB4P95_RS19525 WP_042557262.1 4243284..4244348(-) (pilM) [Pseudomonas sp. A1437]
GTGCTGGGACTCTTCAATAAAAAGACCAATGCGTTACTGGGGATCGACATCAGCTCCACGTCCGTGAAGCTGCTGGAACT
TAGTCGTCAGGGTGATCGCTACCGGGTCGAGGCTTATGCGGTTGAGCCGCTGCCACCGAACGCCGTGGTCGAGAAAAACA
TCGCCGAGCTCGAGGGTGTGGGCCAGGCCCTGACCCGGGTGCTGGGCAAGGCACGCACCGGGCTGAGAAGCGTGGCGGTG
GCGGTGGCCGGTTCAGCTGTGATTACCAAGATCATCGAGATGGACGCCGGGCTTTCCGACGACGAACTGGAAAACCAGCT
CAAGATTGAAGCCGACCAATACATTCCCTATCCGCTGGACGAAGTCGCCATCGACTTCGAGGTTCAGGGCGTGTCGCCGC
GCAACCCCGAGCGGGTCAACGTGTTGCTGGCTGCCTGTCGCAAGGAAAACGTCGAAGTCCGTGAAGCGGCACTGGCGCTG
GCCGGGTTGACCGCGCGCGTGGTGGACGTCGAAGCGTACGCGCTGGAGCGTTCGTTCGGCTTGCTCGCCACGCAGCTGGC
GGCTTCTCAAGAGCGCTTGACCGTGGCGGTGGTCGACATCGGCGCCACCATGACCACCCTCAGCGTCCTGCACAACGGCA
AGATCATCTACACCCGTGAGCAGTTGTTCGGCGGCCGGCAACTGACCGAGGAGATCCAGCGTCGCTATGGCCTGACCGTC
GAGCAGGCGGGGCTGGCAAAGAAGCAGGGCGGTCTGCCCGACGATTACGTCAGCGAGGTGTTGCAGCCGTTTCGCGAGGC
GCTGGTGCAGCAGGTATCGCGTTCGTTGCAGTTTTTCTTCGCTTCCGGCCAGTACAACGCGGTCGACCATATTCTGCTCG
CTGGCGGCACGGCGTCGGTGCCGGGGCTGGATCGGCTGATCGAACAGCGCCTGGATACTCCGACCCAAGTGGCCAATCCG
TTTTCCAATATGGCCCTGAGCAGCAAGGTCAACGCCGGGGCGCTGGCCAGTGACGCGCCGGCCTTGATGATCGCCTGCGG
GCTCGCGCTCAGGAGTTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

56.497

100

0.565
  comM Acinetobacter nosocomialis M2

56.497

100

0.565
  comM Acinetobacter baylyi ADP1

55.367

100

0.554
  pilM Legionella pneumophila strain ERS1305867

46.893

100

0.469

Multiple sequence alignment