Detailed information NTG1026870

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: AB3471_RS02060
• Genome accession: NZ_CP162520
• Coordinates: 442207..443271 (+)
• Length: 354 a.a.
• NCBI ID: WP_003341394.1
• Uniprot ID: A0A0P9GH14
• Organism: Pseudomonas syringae pv. atrofaciens strain UPB 463
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 437207..448271

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
AB3471_RS02050 (AB3471_02040)	-	437461..438729 (+)	1269	WP_003316648.1	malic enzyme-like NAD(P)-binding protein	-
AB3471_RS02055 (AB3471_02045)	-	439556..441997 (-)	2442	WP_375232557.1	penicillin-binding protein 1A	-
AB3471_RS02060 (AB3471_02050)	pilM	442207..443271 (+)	1065	WP_003341394.1	pilus assembly protein PilM	Machinery gene
AB3471_RS02065 (AB3471_02055)	-	443271..443852 (+)	582	WP_003316651.1	PilN domain-containing protein	-
AB3471_RS02070 (AB3471_02060)	pilO	443849..444472 (+)	624	WP_003316652.1	type 4a pilus biogenesis protein PilO	-
AB3471_RS02075 (AB3471_02065)	pilP	444469..444996 (+)	528	WP_003316653.1	type 4a pilus biogenesis lipoprotein PilP	-
AB3471_RS02080 (AB3471_02070)	pilQ	445044..447149 (+)	2106	WP_003422532.1	type IV pilus secretin PilQ	Machinery gene
AB3471_RS02085 (AB3471_02075)	aroK	447154..447672 (+)	519	WP_003341387.1	shikimate kinase AroK	-

Sequence

Protein

• Length: 354 a.a.
• Molecular weight: 38134.68 Da
• Isoelectric Point: 4.7090

>NTDB_id=1026870 AB3471_RS02060 WP_003341394.1 442207..443271(+) (pilM) [Pseudomonas syringae pv. atrofaciens strain UPB 463]
MFELFSKKANTLLGIDISSTSVKLLELSRSGTRYKVESYAVEPLPANAVVEKNIAELEGVGQALSRVLVKAKTSVKIVAV
AVAGSAVITKTIEMDAGLSDDDMENQLKLEADQYIPYPLEEVAIDFEVQGYSVRNPERVEVLLAACRKENVEVREAALAL
AGLTARVVDVEAYALERSFGLLAAQLGNGHDELTVAVVDIGATMTTLSVLHHGRIIYTREQLFGGRQLTDEIQRRYGLSM
EEAGLAKKQGGLPDDYVSEVLEPFKEALVQQVSRSLQFFFAAGQYNSVDHIMLAGGTASISGLEHLIQRRIGTPTMVANP
FADMALSSKVNAGALASDAPALMIACGLALRSFD

Nucleotide

• Length: 1065 bp

>NTDB_id=1026870 AB3471_RS02060 WP_003341394.1 442207..443271(+) (pilM) [Pseudomonas syringae pv. atrofaciens strain UPB 463]
GTGTTCGAACTCTTCAGTAAGAAGGCCAACACCCTTCTAGGGATCGATATTAGCTCCACCTCGGTAAAACTCCTGGAATT
GAGTCGTTCCGGCACCCGTTACAAGGTCGAGTCTTACGCAGTAGAGCCATTGCCGGCCAACGCGGTCGTCGAAAAGAACA
TTGCCGAACTCGAAGGGGTCGGGCAGGCGTTGTCACGCGTGCTCGTCAAGGCCAAGACCAGCGTCAAGATCGTGGCGGTT
GCCGTGGCCGGTTCGGCGGTGATCACCAAGACCATCGAGATGGACGCCGGGCTTTCCGACGATGACATGGAAAACCAGCT
CAAGCTTGAAGCTGACCAGTACATCCCCTATCCGCTGGAAGAAGTCGCCATCGATTTCGAAGTGCAGGGCTATTCGGTGC
GCAACCCCGAGCGCGTCGAGGTGTTACTGGCTGCCTGCCGCAAGGAAAACGTCGAAGTACGCGAGGCCGCGCTGGCACTG
GCCGGGCTGACCGCACGCGTCGTCGATGTCGAGGCCTACGCCCTTGAGCGTTCGTTCGGTTTGCTGGCGGCGCAACTGGG
CAATGGCCATGACGAGCTGACGGTGGCCGTGGTCGATATCGGCGCCACCATGACCACTCTGAGCGTGCTGCATCATGGCC
GGATCATCTATACCCGCGAGCAGCTGTTTGGTGGGCGCCAGTTGACTGACGAAATCCAGCGCCGCTACGGGCTCTCCATG
GAGGAAGCCGGGCTTGCCAAGAAGCAGGGCGGCTTGCCCGACGATTACGTCAGCGAGGTGCTCGAGCCCTTCAAGGAAGC
GCTGGTGCAGCAGGTTTCCCGATCCCTGCAGTTCTTCTTCGCGGCCGGTCAATACAACTCAGTGGATCACATCATGCTGG
CAGGCGGCACGGCCTCGATTTCAGGGCTTGAGCATCTGATCCAGCGGCGCATCGGCACGCCGACCATGGTCGCCAACCCG
TTTGCCGACATGGCACTGAGCTCCAAGGTCAATGCCGGCGCCCTGGCCAGTGATGCGCCGGCGCTGATGATCGCGTGCGG
CCTGGCGTTGAGGAGCTTCGACTGA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-353)

3D structure

• AlphaFold DB: A0A0P9GH14

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	55.65	100	0.557
comM	Acinetobacter nosocomialis M2	55.65	100	0.557
comM	Acinetobacter baylyi ADP1	54.237	100	0.542
pilM	Legionella pneumophila strain ERS1305867	46.761	100	0.469