Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ABZP26_RS13585 Genome accession   NZ_CP162514
Coordinates   2903830..2904966 (-) Length   378 a.a.
NCBI ID   WP_013465850.1    Uniprot ID   A0AA37S1K5
Organism   Pseudoalteromonas sp. SD03     
Function   mediate the depolymerization of the type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2898830..2909966
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABZP26_RS13555 (ABZP26_13555) - 2899346..2900239 (+) 894 WP_013465844.1 hypothetical protein -
  ABZP26_RS13560 (ABZP26_13560) - 2900317..2900790 (+) 474 WP_368485068.1 hypothetical protein -
  ABZP26_RS13565 (ABZP26_13565) rsmE 2900861..2901595 (+) 735 WP_013465846.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  ABZP26_RS13570 (ABZP26_13570) gshB 2901662..2902612 (+) 951 WP_013465847.1 glutathione synthase -
  ABZP26_RS13575 (ABZP26_13575) - 2902748..2903305 (+) 558 WP_024600819.1 YqgE/AlgH family protein -
  ABZP26_RS13580 (ABZP26_13580) ruvX 2903323..2903772 (+) 450 WP_033102541.1 Holliday junction resolvase RuvX -
  ABZP26_RS13585 (ABZP26_13585) pilU 2903830..2904966 (-) 1137 WP_013465850.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ABZP26_RS13590 (ABZP26_13590) pilT 2904976..2906016 (-) 1041 WP_013465851.1 type IV pilus twitching motility protein PilT Machinery gene
  ABZP26_RS13595 (ABZP26_13595) - 2906019..2906732 (+) 714 WP_128583160.1 YggS family pyridoxal phosphate-dependent enzyme -
  ABZP26_RS13600 (ABZP26_13600) proC 2906750..2907571 (+) 822 WP_024600822.1 pyrroline-5-carboxylate reductase -
  ABZP26_RS13605 (ABZP26_13605) - 2907598..2908131 (+) 534 WP_008113334.1 YggT family protein -
  ABZP26_RS13610 (ABZP26_13610) - 2908190..2908633 (+) 444 WP_128583159.1 DUF4426 domain-containing protein -
  ABZP26_RS13615 (ABZP26_13615) - 2908636..2909235 (+) 600 WP_069439858.1 XTP/dITP diphosphatase -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 41994.04 Da        Isoelectric Point: 6.2895

>NTDB_id=1026720 ABZP26_RS13585 WP_013465850.1 2903830..2904966(-) (pilU) [Pseudoalteromonas sp. SD03]
MTLSLNHFLQIMIDKKGSDLFVSSQLPISAKINGELTPLNDDKLTDEQALELVESAMSEKQKAEFHNTKECNFAIATSEG
RFRISAFWQRDRAGMVIRRIVTKIPDVKELGLPSALTDVIMSKRGLVLFVGGTGTGKSTSLAALIGYRNRNQRGHILTIE
DPIEFVHEHQKSIITQREVGLDTESFESALKSSLRQAPDVILIGEIRSQETMEYALSFAETGHLCVATLHANNANQAIDR
IMHLVPKEKHDKLKYDLALNLRAIIAQQLIPTSDGEGRVAAIEVLLNTPMVSELIKNGNIGGIKETMAKSTEMGMQTFDQ
ALFELYRQQRINYADALHHADSPNDLRLMIKLRNNEQQGAGFLQGVTIDGLDDKGNMT

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=1026720 ABZP26_RS13585 WP_013465850.1 2903830..2904966(-) (pilU) [Pseudoalteromonas sp. SD03]
ATGACTTTATCTTTAAATCACTTTTTACAAATAATGATCGACAAAAAAGGCTCTGATTTATTTGTATCGAGCCAACTTCC
TATTAGCGCTAAAATTAACGGCGAGCTAACTCCTCTTAATGACGATAAATTAACAGATGAGCAAGCGCTAGAGTTAGTTG
AATCAGCGATGAGTGAAAAACAAAAAGCAGAGTTTCATAACACTAAAGAATGTAACTTTGCGATTGCAACTAGCGAAGGG
CGCTTTCGTATTTCGGCTTTTTGGCAACGCGACAGAGCGGGCATGGTTATTCGCCGAATCGTCACTAAGATTCCCGACGT
TAAAGAGCTTGGATTACCTTCTGCATTGACCGATGTGATTATGTCAAAGCGCGGACTTGTGTTATTTGTAGGTGGTACGG
GAACCGGTAAATCAACGTCTTTGGCGGCACTTATTGGTTATAGAAATCGCAATCAACGTGGGCATATTTTAACCATAGAA
GATCCGATTGAATTTGTGCATGAACATCAAAAAAGTATTATTACTCAACGTGAAGTGGGTTTAGATACTGAAAGCTTTGA
ATCGGCACTTAAAAGCTCTTTGCGCCAAGCGCCTGATGTTATTTTAATTGGTGAAATTCGTTCGCAAGAAACCATGGAAT
ATGCGTTGAGCTTTGCAGAAACGGGTCATTTATGTGTTGCCACCTTGCATGCTAACAATGCTAACCAAGCCATTGACCGC
ATTATGCACTTAGTACCAAAAGAAAAGCACGATAAGCTTAAATATGACTTAGCATTAAATTTACGCGCTATCATTGCTCA
GCAACTTATTCCTACCTCTGATGGGGAAGGGCGTGTTGCTGCCATTGAGGTATTACTTAATACGCCAATGGTCAGTGAGC
TAATTAAAAACGGTAATATTGGTGGCATTAAAGAAACCATGGCTAAATCAACAGAGATGGGCATGCAAACCTTTGACCAA
GCGCTGTTTGAGCTTTACAGACAGCAACGTATTAATTATGCCGATGCGCTGCATCATGCGGATTCGCCAAATGACTTACG
CTTGATGATTAAACTACGTAATAACGAGCAACAAGGCGCAGGCTTCTTACAAGGTGTGACCATTGATGGCCTTGATGACA
AAGGCAATATGACTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(14-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Acinetobacter baylyi ADP1

57.56

99.735

0.574
  pilU Pseudomonas stutzeri DSM 10701

57.6

99.206

0.571
  pilU Vibrio cholerae strain A1552

56.131

97.09

0.545
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.944

95.238

0.399

Multiple sequence alignment