Detailed information NTG1024928

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: AB3W23_RS02030
• Genome accession: NZ_CP162336
• Coordinates: 438247..439395 (+)
• Length: 382 a.a.
• NCBI ID: WP_003100959.1
• Uniprot ID: G3XCX3
• Organism: Pseudomonas aeruginosa strain 0303d1.1
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 433247..444395

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
AB3W23_RS02005 (AB3W23_02005)	-	434398..434694 (-)	297	WP_031628111.1	DUF167 domain-containing protein	-
AB3W23_RS02010 (AB3W23_02010)	-	434691..435284 (-)	594	WP_003084544.1	YggT family protein	-
AB3W23_RS02015 (AB3W23_02015)	proC	435295..436116 (-)	822	WP_003084546.1	pyrroline-5-carboxylate reductase	-
AB3W23_RS02020 (AB3W23_02020)	-	436128..436820 (-)	693	WP_003084549.1	YggS family pyridoxal phosphate-dependent enzyme	-
AB3W23_RS02025 (AB3W23_02025)	pilT	437035..438069 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
AB3W23_RS02030 (AB3W23_02030)	pilU	438247..439395 (+)	1149	WP_003100959.1	type IV pilus ATPase PilU	Machinery gene
AB3W23_RS02035 (AB3W23_02035)	czcD	439403..440302 (-)	900	WP_003084557.1	CDF family zinc transporter CzcD	-
AB3W23_RS02040 (AB3W23_02040)	-	440457..440861 (+)	405	WP_003084560.1	NINE protein	-
AB3W23_RS02045 (AB3W23_02045)	-	441104..442477 (+)	1374	WP_003084562.1	pyridoxal-phosphate dependent enzyme	-
AB3W23_RS02050 (AB3W23_02050)	-	442474..443658 (+)	1185	WP_003084564.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42532.81 Da
• Isoelectric Point: 6.7792

>NTDB_id=1024928 AB3W23_RS02030 WP_003100959.1 438247..439395(+) (pilU) [Pseudomonas aeruginosa strain 0303d1.1]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSSLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=1024928 AB3W23_RS02030 WP_003100959.1 438247..439395(+) (pilU) [Pseudomonas aeruginosa strain 0303d1.1]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAATTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACTCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAGCCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: G3XCX3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.482	100	0.885
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382