Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   AB3W22_RS27145 Genome accession   NZ_CP162335
Coordinates   5836954..5838018 (-) Length   354 a.a.
NCBI ID   WP_034024943.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain 1001d0_1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5831954..5843018
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AB3W22_RS27120 (AB3W22_27120) aroK 5832501..5833019 (-) 519 WP_003095833.1 shikimate kinase AroK -
  AB3W22_RS27125 (AB3W22_27125) pilQ 5833031..5835163 (-) 2133 WP_003116137.1 type 4a pilus secretin PilQ Machinery gene
  AB3W22_RS27130 (AB3W22_27130) pilP 5835217..5835741 (-) 525 WP_003116136.1 type 4a pilus biogenesis lipoprotein PilP -
  AB3W22_RS27135 (AB3W22_27135) pilO 5835738..5836361 (-) 624 WP_003116135.1 type 4a pilus biogenesis protein PilO -
  AB3W22_RS27140 (AB3W22_27140) pilN 5836358..5836954 (-) 597 WP_003141783.1 type 4a pilus biogenesis protein PilN -
  AB3W22_RS27145 (AB3W22_27145) pilM 5836954..5838018 (-) 1065 WP_034024943.1 type IV pilus assembly protein PilM Machinery gene
  AB3W22_RS27150 (AB3W22_27150) - 5838203..5840671 (+) 2469 WP_003114576.1 penicillin-binding protein 1A -
  AB3W22_RS27155 (AB3W22_27155) - 5840776..5842044 (-) 1269 WP_003103278.1 malic enzyme -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 37997.51 Da        Isoelectric Point: 4.5446

>NTDB_id=1024921 AB3W22_RS27145 WP_034024943.1 5836954..5838018(-) (pilM) [Pseudomonas aeruginosa strain 1001d0_1]
MLGLIKKKANTLLGIDISSTSVKLLELSRSGGRYKVEAYAVEPLPPNAVVEKNIVELEGVGQALSRVLVKAKTNLKSAVV
AVAGSAVITKTIEMEAGLSEDELENQLKIEADQYIPYPLEEVAIDFEVQGLSARNPERVEVLLAACRKENVEVREAALAL
AGLTAKVVDVEAYALERSYALLSSQLGADTDQLTVAVVDIGATMTTLSVLHNGRTIYTREQLFGGRQLTEEIQRRYGLSV
EEAGLAKKQGGLPDDYDSEVLRPFKDAVVQQVSRSLQFFFAAGQFNDVDYIVLAGGTASIQDLDRLIQQKIGTPTLVANP
FADMALNGKVNAGALASDAPALMIACGLALRSFD

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=1024921 AB3W22_RS27145 WP_034024943.1 5836954..5838018(-) (pilM) [Pseudomonas aeruginosa strain 1001d0_1]
GTGCTAGGGCTCATAAAGAAGAAAGCGAACACCTTGCTGGGGATCGACATCAGCTCGACCTCGGTAAAGCTCCTTGAGTT
GAGCCGCTCCGGAGGCCGCTACAAAGTGGAGGCCTATGCCGTCGAGCCGCTTCCGCCGAATGCAGTCGTGGAAAAGAACA
TCGTCGAGCTGGAAGGGGTCGGCCAGGCGTTGTCACGGGTGCTGGTCAAGGCGAAAACCAACCTGAAGTCGGCCGTGGTC
GCCGTCGCCGGTTCGGCGGTGATCACCAAGACCATCGAGATGGAGGCCGGGCTTTCCGAGGATGAACTGGAAAACCAGCT
GAAGATCGAGGCCGACCAGTACATCCCCTATCCGCTGGAGGAAGTCGCCATCGACTTCGAGGTCCAGGGACTCTCCGCGC
GCAATCCCGAGCGCGTGGAGGTACTCCTGGCCGCCTGTCGGAAAGAGAACGTCGAGGTTCGCGAAGCGGCCCTGGCGCTG
GCCGGCCTGACCGCCAAGGTGGTCGACGTCGAGGCCTACGCGCTGGAGCGCTCCTATGCGCTGCTGAGCAGCCAGCTGGG
TGCCGACACCGACCAGTTGACCGTGGCGGTAGTGGACATCGGCGCGACCATGACCACCCTGAGCGTCCTGCACAACGGAC
GCACCATCTATACCCGCGAACAGCTGTTCGGCGGTCGCCAGCTCACCGAGGAGATCCAGCGGCGTTACGGACTCTCGGTG
GAGGAAGCCGGTCTCGCCAAGAAGCAGGGCGGTCTTCCGGATGACTACGACAGCGAAGTTCTGCGTCCGTTCAAGGACGC
CGTGGTGCAGCAGGTTTCCCGCTCGCTGCAGTTCTTCTTCGCCGCCGGACAGTTCAACGACGTCGACTACATCGTCCTGG
CGGGCGGTACGGCGTCTATCCAGGATCTCGATCGGTTGATCCAGCAGAAGATCGGCACCCCGACCCTGGTCGCCAACCCG
TTCGCCGACATGGCGCTGAACGGCAAGGTGAATGCCGGCGCCCTGGCCAGCGACGCTCCGGCGTTGATGATCGCCTGCGG
CCTGGCGTTGAGGAGTTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

55.899

100

0.562
  comM Acinetobacter nosocomialis M2

55.899

100

0.562
  comM Acinetobacter baylyi ADP1

51.977

100

0.52
  pilM Legionella pneumophila strain ERS1305867

46.328

100

0.463

Multiple sequence alignment