Detailed information    

insolico Bioinformatically predicted

Overview

Name   comE   Type   Machinery gene
Locus tag   AB3F25_RS08120 Genome accession   NZ_CP162010
Coordinates   1720130..1721503 (-) Length   457 a.a.
NCBI ID   WP_373604355.1    Uniprot ID   -
Organism   Aggregatibacter sp. HMT-949 strain W10649     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1715130..1726503
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AB3F25_RS08090 (AB3F25_08085) - 1715224..1715319 (-) 96 WP_373603332.1 methionine/alanine import family NSS transporter small subunit -
  AB3F25_RS08095 (AB3F25_08090) - 1715321..1716835 (-) 1515 WP_373603333.1 sodium-dependent transporter -
  AB3F25_RS08100 (AB3F25_08095) asnA 1717078..1718070 (-) 993 WP_373603334.1 aspartate--ammonia ligase -
  AB3F25_RS08105 (AB3F25_08100) asnC 1718227..1718679 (+) 453 WP_373603335.1 transcriptional regulator AsnC -
  AB3F25_RS08110 (AB3F25_08105) nfuA 1718744..1719328 (-) 585 WP_373603336.1 Fe-S biogenesis protein NfuA -
  AB3F25_RS08115 (AB3F25_08110) - 1719429..1720115 (-) 687 WP_373603337.1 amidophosphoribosyltransferase -
  AB3F25_RS08120 (AB3F25_08115) comE 1720130..1721503 (-) 1374 WP_373604355.1 type IV pilus secretin PilQ Machinery gene
  AB3F25_RS08125 (AB3F25_08120) - 1721524..1721919 (-) 396 WP_373603338.1 pilus assembly protein PilP -
  AB3F25_RS08130 (AB3F25_08125) - 1721922..1722437 (-) 516 WP_373603339.1 hypothetical protein -
  AB3F25_RS08135 (AB3F25_08130) - 1722434..1722952 (-) 519 WP_373603340.1 PilN domain-containing protein -
  AB3F25_RS08140 (AB3F25_08135) - 1722939..1723769 (-) 831 WP_373603341.1 competence protein ComA -
  AB3F25_RS08145 (AB3F25_08140) - 1723868..1726492 (+) 2625 WP_373603342.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 457 a.a.        Molecular weight: 50001.29 Da        Isoelectric Point: 5.8363

>NTDB_id=1023497 AB3F25_RS08120 WP_373604355.1 1720130..1721503(-) (comE) [Aggregatibacter sp. HMT-949 strain W10649]
MKKYGLLLLSFCCLKVAAEFKENQVFSIRLKQAPMVASLQELALVQGTNLVVDDELEGSLSLQLDNVDLDRVLRSVARMK
GLSLRVEDDIYYLGKRSEQEAFAAPMNAVPPALVGESVDALKESESNLVSRTIKLHFAKASEVMKSLTTGSGSLLSPAGS
IAFDDRSNLLIIQDEAKSVKNMAKLIAELDKPIEQIAIEARIVTITDESLKELGVRWGIFAPTENAYRVGGSLEAAGFTN
LTENLNVNFAANTTPAGSIALQVAKINGRLLDLELTALERENNVEIIASPRLLTTNKKSASIKQGTEIPYVVTNGKNDTQ
SVEFREAVLGLEVTPHISKDNNILLDLLVSQNSPGNRVSYGQQNNEVVSIDKQEINTQVFAKDGETIVLGGVFHDTISKG
VDKVPVLGDIPLVKRLFSKESERHQKRELVIFVTPHILKQGETLDSLKQKTRGKQGR

Nucleotide


Download         Length: 1374 bp        

>NTDB_id=1023497 AB3F25_RS08120 WP_373604355.1 1720130..1721503(-) (comE) [Aggregatibacter sp. HMT-949 strain W10649]
TTGAAAAAATATGGTCTGCTTTTGTTGAGTTTTTGCTGTTTGAAGGTTGCAGCCGAGTTCAAAGAAAATCAAGTGTTTTC
CATTCGTTTGAAACAGGCCCCGATGGTGGCGAGCTTGCAAGAGCTGGCACTGGTGCAAGGGACGAATTTAGTGGTGGATG
ACGAACTGGAGGGATCGCTTTCTTTGCAATTGGACAATGTGGATCTTGATCGCGTGTTGCGTTCTGTGGCGAGAATGAAA
GGTTTGTCGTTGCGTGTGGAAGATGACATTTATTATTTGGGCAAACGTTCCGAGCAGGAAGCCTTTGCCGCACCGATGAA
TGCGGTGCCACCGGCGTTAGTCGGCGAATCCGTTGACGCGTTGAAAGAATCGGAATCCAATTTGGTCAGCCGTACGATAA
AACTGCATTTCGCCAAAGCATCGGAAGTAATGAAATCCCTTACTACGGGTAGCGGCTCGTTGCTATCGCCGGCGGGTTCG
ATTGCCTTTGATGATCGTAGTAACTTGTTGATTATTCAAGATGAAGCGAAATCCGTCAAAAATATGGCGAAACTGATCGC
AGAGCTTGATAAACCTATTGAACAAATCGCCATTGAGGCGCGTATCGTAACAATTACCGATGAGAGTTTGAAGGAATTAG
GCGTGCGTTGGGGAATCTTTGCACCAACGGAAAACGCTTATCGCGTGGGTGGGAGTTTAGAGGCGGCCGGTTTTACGAAT
TTAACGGAAAATTTAAATGTGAATTTTGCCGCAAATACCACACCGGCCGGTTCAATAGCGCTGCAAGTGGCCAAGATTAA
CGGACGCTTGTTAGACTTGGAACTGACTGCATTGGAGCGTGAAAACAATGTGGAAATTATTGCCAGCCCACGTTTACTAA
CGACTAATAAGAAAAGCGCAAGTATCAAACAAGGGACGGAAATTCCTTATGTGGTCACCAACGGTAAAAACGACACACAA
TCTGTTGAATTCCGTGAAGCGGTACTGGGATTGGAAGTAACGCCGCATATTTCCAAAGATAATAATATTTTATTGGATTT
ATTGGTTAGTCAAAATTCACCAGGCAATCGTGTTTCTTATGGGCAGCAAAATAATGAAGTGGTGTCCATCGACAAGCAAG
AAATTAACACCCAAGTATTTGCTAAAGATGGCGAAACTATCGTATTGGGTGGCGTGTTCCATGACACGATTAGTAAAGGT
GTGGATAAAGTGCCGGTGTTGGGGGATATTCCGCTGGTAAAACGATTGTTCAGTAAAGAAAGCGAACGCCATCAAAAACG
TGAGTTAGTGATTTTTGTGACGCCGCATATTTTGAAACAAGGGGAAACGTTGGATTCCTTAAAACAGAAAACGAGAGGCA
AGCAAGGGCGGTAA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(131-195)

Bacterial type II and III secretion system protein (Secretin)

(277-439)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comE Haemophilus influenzae Rd KW20

72.294

100

0.731
  comE Haemophilus influenzae 86-028NP

71.645

100

0.724
  comE Glaesserella parasuis strain SC1401

53.468

97.812

0.523
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

42.361

94.53

0.4
  pilQ Vibrio cholerae strain A1552

42.361

94.53

0.4
  pilQ Vibrio campbellii strain DS40M4

41.458

96.061

0.398

Multiple sequence alignment