Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   QUE24_RS09740 Genome accession   NZ_AP027741
Coordinates   1893108..1894145 (+) Length   345 a.a.
NCBI ID   WP_286303641.1    Uniprot ID   -
Organism   Methylophaga marina strain NBRC 102421     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1888108..1899145
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QUE24_RS09710 (GCM10025856_19410) pyrE 1888430..1889074 (-) 645 WP_286306104.1 orotate phosphoribosyltransferase -
  QUE24_RS09715 (GCM10025856_19420) metW 1889070..1889672 (-) 603 WP_286303636.1 methionine biosynthesis protein MetW -
  QUE24_RS09720 (GCM10025856_19430) - 1889672..1890820 (-) 1149 WP_286303637.1 homoserine O-acetyltransferase -
  QUE24_RS09725 (GCM10025856_19440) - 1890884..1891453 (-) 570 WP_286303638.1 YggT family protein -
  QUE24_RS09730 (GCM10025856_19450) proC 1891446..1892279 (-) 834 WP_286303639.1 pyrroline-5-carboxylate reductase -
  QUE24_RS09735 (GCM10025856_19460) - 1892328..1893017 (-) 690 WP_286303640.1 YggS family pyridoxal phosphate-dependent enzyme -
  QUE24_RS09740 (GCM10025856_19470) pilT 1893108..1894145 (+) 1038 WP_286303641.1 type IV pilus twitching motility protein PilT Machinery gene
  QUE24_RS09745 (GCM10025856_19480) pilU 1894157..1895299 (+) 1143 WP_286303642.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  QUE24_RS09750 (GCM10025856_19490) - 1895460..1896293 (+) 834 WP_286303644.1 OmpA family protein -
  QUE24_RS09755 (GCM10025856_19500) - 1896881..1898653 (-) 1773 WP_286306105.1 methanol/ethanol family PQQ-dependent dehydrogenase -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38391.03 Da        Isoelectric Point: 6.7279

>NTDB_id=102229 QUE24_RS09740 WP_286303641.1 1893108..1894145(+) (pilT) [Methylophaga marina strain NBRC 102421]
MDITELLAFSAKNNASDLHVSSGEPPMIRVDGDIKRINLPAMEHKDVHAMIYDIMNDKQRKDFEEFLEADFSFEVPGLAR
FRVNAFNQNRGAAAVFRTIPSKVLSLEDLGAPDIFKTIADNHRGIVLVTGPTGSGKSTTLAGMINYRNEKDNEHILTIED
PIEFVHQSKKCLINQREVHRDTHGFSEALRSALREDPDIILVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPAAEKDMVRSMLSESLRAVISQTLMKKVGGGRIAAHEIMIGTPAIRNLIREDKVPQMYSAIQTGGAIGMQTLDQCL
QDLVRRQKVTKQEALPRAVNKDLFR

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=102229 QUE24_RS09740 WP_286303641.1 1893108..1894145(+) (pilT) [Methylophaga marina strain NBRC 102421]
ATGGATATTACTGAATTGCTTGCGTTCAGCGCAAAAAACAATGCCTCGGATTTACATGTTTCATCAGGTGAGCCGCCAAT
GATCCGTGTCGATGGTGATATCAAGCGCATTAACCTGCCAGCGATGGAACATAAAGATGTTCACGCGATGATTTATGACA
TTATGAATGACAAACAGCGCAAGGACTTTGAAGAGTTCCTGGAAGCTGATTTCTCATTTGAGGTGCCGGGCTTGGCACGT
TTTCGTGTCAATGCTTTCAATCAGAACCGCGGTGCGGCGGCTGTTTTTAGAACGATTCCATCCAAAGTATTGAGTCTTGA
AGATCTTGGCGCTCCCGATATTTTTAAAACCATAGCGGATAATCATCGCGGAATTGTATTGGTGACTGGGCCAACCGGTT
CGGGTAAATCCACGACGCTGGCAGGCATGATTAATTATCGAAATGAAAAAGATAATGAACATATTTTAACCATTGAAGAC
CCGATCGAATTCGTACATCAAAGTAAAAAATGTTTGATCAATCAGCGTGAAGTGCATCGTGATACTCATGGTTTTAGTGA
AGCGCTTAGAAGTGCTTTGCGTGAAGATCCAGATATCATTCTGGTAGGTGAGTTACGTGATCTGGAGACAATTAGATTGG
CGTTGACAGCCGCTGAAACCGGGCATTTAGTCTTTGGTACCTTACATACCTCGTCTGCAGCAAAAACCATTGACCGCATC
ATTGACGTGTTCCCTGCTGCGGAAAAAGATATGGTGCGTTCCATGTTATCTGAATCATTGCGCGCGGTTATTTCGCAAAC
ATTAATGAAAAAAGTCGGCGGTGGTCGAATTGCTGCGCATGAAATCATGATTGGCACGCCTGCCATCAGAAATCTAATAC
GCGAAGATAAAGTACCGCAGATGTACTCTGCTATTCAGACGGGCGGCGCTATTGGTATGCAAACGTTAGATCAGTGTTTA
CAAGATTTGGTCAGAAGACAAAAAGTGACAAAACAGGAAGCATTACCACGTGCTGTTAATAAAGACTTATTCCGTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

79.651

99.71

0.794
  pilT Acinetobacter nosocomialis M2

79.07

99.71

0.788
  pilT Acinetobacter baumannii D1279779

79.07

99.71

0.788
  pilT Acinetobacter baumannii strain A118

79.07

99.71

0.788
  pilT Pseudomonas aeruginosa PAK

79.882

97.971

0.783
  pilT Acinetobacter baylyi ADP1

76.833

98.841

0.759
  pilT Legionella pneumophila strain Lp02

74.128

99.71

0.739
  pilT Legionella pneumophila strain ERS1305867

74.128

99.71

0.739
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

71.471

96.522

0.69
  pilT Vibrio cholerae strain A1552

71.471

96.522

0.69
  pilT Neisseria meningitidis 8013

69.096

99.42

0.687
  pilT Neisseria gonorrhoeae MS11

68.805

99.42

0.684
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

48.986

100

0.49
  pilU Acinetobacter baylyi ADP1

41.765

98.551

0.412
  pilU Pseudomonas stutzeri DSM 10701

41.298

98.261

0.406
  pilU Vibrio cholerae strain A1552

40.413

98.261

0.397

Multiple sequence alignment