Detailed information NTG1019554

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: AB0R89_RS12365
• Genome accession: NZ_CP160218
• Coordinates: 2615610..2616224 (-)
• Length: 204 a.a.
• NCBI ID: WP_043021637.1
• Uniprot ID: -
• Organism: Bacillus velezensis strain AP45
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2610610..2621224

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
AB0R89_RS12335 (AB0R89_12335)	rpsT	2610913..2611179 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
AB0R89_RS12340 (AB0R89_12340)	holA	2611196..2612237 (-)	1042	Protein_2383	DNA polymerase III subunit delta	-
AB0R89_RS12345 (AB0R89_12345)	-	2612277..2612428 (-)	152	Protein_2384	hypothetical protein	-
AB0R89_RS12350 (AB0R89_12350)	-	2612469..2612603 (+)	135	WP_003152870.1	YqzM family protein	-
AB0R89_RS12355 (AB0R89_12355)	comEC	2612622..2614973 (-)	2352	WP_043021636.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
AB0R89_RS12360 (AB0R89_12360)	-	2614974..2615543 (-)	570	WP_003152868.1	ComE operon protein 2	-
AB0R89_RS12365 (AB0R89_12365)	comEA	2615610..2616224 (-)	615	WP_043021637.1	helix-hairpin-helix domain-containing protein	Machinery gene
AB0R89_RS12370 (AB0R89_12370)	comER	2616283..2617104 (+)	822	WP_043021638.1	late competence protein ComER	-
AB0R89_RS12375 (AB0R89_12375)	-	2617173..2617910 (-)	738	WP_015417856.1	class I SAM-dependent methyltransferase	-
AB0R89_RS12380 (AB0R89_12380)	rsfS	2617907..2618263 (-)	357	WP_007408260.1	ribosome silencing factor	-
AB0R89_RS12385 (AB0R89_12385)	yqeK	2618281..2618841 (-)	561	WP_014418422.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
AB0R89_RS12390 (AB0R89_12390)	-	2618831..2619400 (-)	570	WP_043021639.1	nicotinate-nucleotide adenylyltransferase	-
AB0R89_RS12395 (AB0R89_12395)	yhbY	2619411..2619701 (-)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-
AB0R89_RS12400 (AB0R89_12400)	aroE	2619695..2620537 (-)	843	WP_043021640.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22165.31 Da
• Isoelectric Point: 6.4888

>NTDB_id=1019554 AB0R89_RS12365 WP_043021637.1 2615610..2616224(-) (comEA) [Bacillus velezensis strain AP45]
MTKAMMTERLRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTAADKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTAAGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=1019554 AB0R89_RS12365 WP_043021637.1 2615610..2616224(-) (comEA) [Bacillus velezensis strain AP45]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCGGTTTT
TATCTGCATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCTGTGCTCGGCCAAACAGCCTCAGCAT
CCGTCGCGGACAAAAAAGACACCGCAGCCGATAAGATTATGATTGATGTGAAAGGCGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGGGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAG
CAGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCGATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAGAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (141-202)

• SLBB domain (SLBB) (68-122)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.854	94.118	0.554
comEA	Latilactobacillus sakei subsp. sakei 23K	32.759	100	0.373
comEA/celA/cilE	Streptococcus mitis NCTC 12261	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae Rx1	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae D39	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae R6	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	35.545	100	0.368
comEA/celA/cilE	Streptococcus mitis SK321	35.071	100	0.363