Detailed information NTG1018248

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: AB0962_RS15335
• Genome accession: NZ_CP160130
• Coordinates: 3140010..3141128 (+)
• Length: 372 a.a.
• NCBI ID: WP_000347036.1
• Uniprot ID: A0A009PAD6
• Organism: Acinetobacter baumannii strain AB37-AUFP
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3135010..3146128

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
AB0962_RS15325 (AB0962_15315)	-	3138126..3138818 (-)	693	WP_032050220.1	YggS family pyridoxal phosphate-dependent enzyme	-
AB0962_RS15330 (AB0962_15320)	pilT	3138945..3139982 (+)	1038	WP_000355489.1	type IV pilus twitching motility protein PilT	Machinery gene
AB0962_RS15335 (AB0962_15325)	pilU	3140010..3141128 (+)	1119	WP_000347036.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
AB0962_RS15340 (AB0962_15330)	fur	3141198..3141635 (-)	438	WP_001122847.1	ferric iron uptake transcriptional regulator	-
AB0962_RS15345 (AB0962_15335)	-	3141747..3142145 (+)	399	WP_001170994.1	outer membrane protein assembly factor BamE	-
AB0962_RS15350 (AB0962_15340)	-	3142200..3142523 (-)	324	WP_000442589.1	RnfH family protein	-
AB0962_RS15355 (AB0962_15345)	-	3142523..3143590 (-)	1068	WP_031962994.1	membrane protein	-
AB0962_RS15360 (AB0962_15350)	-	3143743..3144198 (-)	456	WP_000782976.1	bacteriohemerythrin	-
AB0962_RS15365 (AB0962_15355)	-	3144451..3145299 (-)	849	WP_000336554.1	class II glutamine amidotransferase	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41905.11 Da
• Isoelectric Point: 6.6944

>NTDB_id=1018248 AB0962_RS15335 WP_000347036.1 3140010..3141128(+) (pilU) [Acinetobacter baumannii strain AB37-AUFP]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRKEFAETRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLADEGPDQLSDGKQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=1018248 AB0962_RS15335 WP_000347036.1 3140010..3141128(+) (pilU) [Acinetobacter baumannii strain AB37-AUFP]
ATGGATTTTAATGACTTGCTCAACCTGATGGTTGAAAAAAAATCATCGGATCTTTTTATTACAGATGGCGTTGCGCCTTC
TATGAAGATTAACGGGCAAATTGTTCCAATTTCAAAAAATAGTCTTTCTGGGGAAGTGATTGGTCAACTGTTACATTCCA
TCATGAGTGAAAAACAACGCAAAGAATTTGCAGAAACTCGTGAATGTAACTTTGCCATTATGAACCGTGAAAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAGCGCGACATGCCAGGCATGGTACTACGTCGTATTGAAACCAAGATTCCTTCAAT
TGATGACTTGCAGTTACCGCCTGTACTTAAAGATTTATCGATGACCAAACGCGGCATTATTATTTTCGTAGGCGCGACAG
GTACAGGTAAATCCACTTCATTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAGGGCCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCACGAACATGCAGGTTGCATCATTACTCAGCGTGAAGTCGGGATCGATACCGACTCATTTGA
AATTGCCTTGAAAAATACACTACGACAAGCGCCGGATGTGATCTTAATTGGTGAGATCCGTTCTCGTGAAGTCATGGACT
ATGCGATTGGCTTTGCTGAAACAGGTCACCTTGTATTAGCTACCATGCACGCCAACAACGCCAACCAAGCACTCGACCGT
ATTATTCACTTTTTTGAGAGTGACCGTCATAGTCAGCTTTACATGGACTTGTCACTTAACTTAAAGGCAATGATTGCGCA
GCAGCTCATTCCAACACCAGATGGTAATTCACGCCGTGCAGCAATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAAGATTTGATGAAACGCTCACGTGAACTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATATAAAGCTGGTCAAATTACCTACAAAGATGCGCTTAAACATGCTGATTCACCGAACGATTTGCG
TTTAACCATTAAACTTGCAGATGAAGGTCCCGATCAATTATCAGACGGGAAACAGCATTTAACTTTTGATCGTCAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (31-270)

3D structure

• AlphaFold DB: A0A009PAD6

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.926	100	0.772
pilU	Pseudomonas stutzeri DSM 10701	69.293	98.925	0.685
pilU	Vibrio cholerae strain A1552	53.243	99.462	0.53
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.197	93.011	0.392