Detailed information NTG1018031

Bioinformatically predicted

Overview

• Name: ruvB
• Type: Machinery gene
• Locus tag: DQL28_RS00335
• Genome accession: NZ_LS483334
• Coordinates: 53901..54899 (+)
• Length: 332 a.a.
• NCBI ID: WP_011054100.1
• Uniprot ID: P0DF51
• Organism: Streptococcus pyogenes strain NCTC12050
• Function: homologous recombination (predicted from homology) Homologous recombination

Genomic Context

Location: 48901..59899

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
DQL28_RS00320 (NCTC12050_00064)	-	49904..51322 (+)	1419	WP_111676036.1	hypothetical protein	-
DQL28_RS00325 (NCTC12050_00065)	purB	51342..52634 (+)	1293	WP_030126529.1	adenylosuccinate lyase	-
DQL28_RS00330 (NCTC12050_00066)	comR	52764..53675 (+)	912	WP_002986681.1	transcriptional regulator Rgg4/ComR	Regulator
DQL28_RS09705 (NCTC12050_00067)	comS	53759..53854 (+)	96	WP_198462994.1	quorum-sensing system DWW-type pheromone	Regulator
DQL28_RS00335 (NCTC12050_00068)	ruvB	53901..54899 (+)	999	WP_011054100.1	Holliday junction branch migration DNA helicase RuvB	Machinery gene
DQL28_RS00340 (NCTC12050_00069)	-	55037..55474 (+)	438	WP_002994668.1	low molecular weight protein-tyrosine-phosphatase	-
DQL28_RS00345 (NCTC12050_00070)	-	55497..55898 (+)	402	WP_012560369.1	MORN repeat-containing protein	-
DQL28_RS00350 (NCTC12050_00071)	-	55895..57670 (+)	1776	WP_002987732.1	acyltransferase family protein	-

Sequence

Protein

• Length: 332 a.a.
• Molecular weight: 37566.10 Da
• Isoelectric Point: 5.1632

>NTDB_id=1018031 DQL28_RS00335 WP_011054100.1 53901..54899(+) (ruvB) [Streptococcus pyogenes strain NCTC12050]
MARILDNDVMGNEEFSDRTLRPQYLHEYIGQDKVKEQFAIFIEAAKRRDESLDHVLLFGPPGLGKTTMAFVIANELGVNL
KQTSGPAVEKAGDLVAILNELEPGDILFIDEIHRMPMSVEEVLYSAMEDFYIDIMIGAGDTSRSIHLDLPPFTLIGATTR
AGMLSNPLRARFGITGHMEYYQEKDLTEIVERTATIFEIKIDHEAARKLACRSRGTPRIANRLLKRVRDYAQIIGDGIIT
AQITDRALTMLDVDREGLDYIDQKILRTMIEMYQGGPVGLGTLSVNIAEERNTVEEMYEPYLIQKGFLMRTRTGRVATQK
AYRHLGYPYQNT

Nucleotide

• Length: 999 bp

>NTDB_id=1018031 DQL28_RS00335 WP_011054100.1 53901..54899(+) (ruvB) [Streptococcus pyogenes strain NCTC12050]
ATGGCTAGAATTTTAGACAATGATGTAATGGGAAATGAGGAGTTTTCGGATCGGACTCTTCGCCCTCAATATCTACACGA
ATATATTGGACAAGATAAAGTTAAGGAACAATTCGCTATTTTTATAGAAGCAGCTAAAAGACGTGACGAATCTTTAGACC
ATGTTTTATTATTTGGACCTCCAGGACTGGGAAAAACAACAATGGCATTTGTGATTGCCAATGAATTGGGAGTTAATTTA
AAGCAAACTTCAGGTCCTGCCGTTGAGAAAGCTGGAGATTTGGTAGCTATTTTAAATGAATTAGAACCAGGGGATATTTT
GTTTATTGATGAAATTCACCGCATGCCAATGTCTGTTGAGGAAGTACTATATAGTGCTATGGAAGACTTCTATATTGATA
TTATGATTGGCGCTGGAGATACTAGTCGTAGTATTCATTTGGATCTGCCTCCATTTACATTGATTGGTGCTACTACACGT
GCAGGCATGTTATCTAATCCCCTCAGAGCTCGTTTTGGTATCACTGGTCACATGGAATACTATCAAGAAAAGGATTTGAC
AGAGATTGTGGAGCGTACAGCTACTATTTTTGAAATAAAAATAGACCATGAAGCTGCACGGAAATTAGCTTGTCGCAGTA
GAGGAACGCCCCGCATTGCTAATCGATTACTAAAACGAGTGCGTGACTATGCTCAAATTATTGGTGATGGCATCATAACG
GCTCAAATAACTGATAGAGCTTTGACTATGTTAGATGTTGACCGCGAAGGTCTCGATTACATTGATCAGAAAATTTTACG
AACGATGATTGAGATGTATCAGGGAGGACCAGTTGGTTTGGGGACATTATCTGTGAATATTGCCGAGGAGCGTAATACAG
TTGAAGAGATGTATGAACCCTACTTAATTCAAAAAGGCTTTCTCATGCGAACACGAACAGGTCGTGTAGCGACGCAAAAG
GCTTATCGTCATCTTGGCTATCCCTATCAAAACACTTAA

Domains

Predicted by InterProScan.

• RuvB C-terminal winged helix domain (WHD_RuvB) (256-325)

• RuvB AAA lid domain (AAA_lid_4) (181-254)

• Holliday junction DNA helicase RuvB P-loop domain (RuvB_N) (20-178)

3D structure

• AlphaFold DB: P0DF51

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
ruvB	Streptococcus pneumoniae R6	84.848	99.398	0.843
ruvB	Streptococcus pneumoniae D39	84.848	99.398	0.843
ruvB	Streptococcus pneumoniae TIGR4	84.545	99.398	0.84
ruvB	Bacillus subtilis subsp. subtilis str. 168	58.824	97.289	0.572
ruvB	Helicobacter pylori 26695	48.936	99.096	0.485
ruvB	Synechocystis sp. PCC 6803	50.16	94.277	0.473