Detailed information NTG1017199

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: AB0T70_RS14730
• Genome accession: NZ_CP160032
• Coordinates: 3083931..3085049 (+)
• Length: 372 a.a.
• NCBI ID: WP_000347036.1
• Uniprot ID: A0A009PAD6
• Organism: Acinetobacter baumannii strain AB44-AUFP
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3078931..3090049

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
AB0T70_RS14720 (AB0T70_14720)	-	3082047..3082739 (-)	693	WP_001108515.1	YggS family pyridoxal phosphate-dependent enzyme	-
AB0T70_RS14725 (AB0T70_14725)	pilT	3082866..3083903 (+)	1038	WP_000355489.1	type IV pilus twitching motility protein PilT	Machinery gene
AB0T70_RS14730 (AB0T70_14730)	pilU	3083931..3085049 (+)	1119	WP_000347036.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
AB0T70_RS14735 (AB0T70_14735)	fur	3085119..3085556 (-)	438	WP_001122847.1	ferric iron uptake transcriptional regulator	-
AB0T70_RS14740 (AB0T70_14740)	bamE	3085668..3086066 (+)	399	WP_001170994.1	outer membrane protein assembly factor BamE	-
AB0T70_RS14745 (AB0T70_14745)	-	3086121..3086444 (-)	324	WP_002069561.1	RnfH family protein	-
AB0T70_RS14750 (AB0T70_14750)	-	3086444..3087511 (-)	1068	WP_010590411.1	membrane protein	-
AB0T70_RS14755 (AB0T70_14755)	-	3087664..3088119 (-)	456	WP_000782976.1	bacteriohemerythrin	-
AB0T70_RS14760 (AB0T70_14760)	-	3088372..3089220 (-)	849	WP_000336554.1	class II glutamine amidotransferase	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41905.11 Da
• Isoelectric Point: 6.6944

>NTDB_id=1017199 AB0T70_RS14730 WP_000347036.1 3083931..3085049(+) (pilU) [Acinetobacter baumannii strain AB44-AUFP]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRKEFAETRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLADEGPDQLSDGKQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=1017199 AB0T70_RS14730 WP_000347036.1 3083931..3085049(+) (pilU) [Acinetobacter baumannii strain AB44-AUFP]
ATGGATTTTAATGACTTGCTCAACCTGATGGTTGAAAAAAAATCTTCCGATCTCTTTATTACAGATGGCGTTGCGCCCTC
TATGAAGATTAACGGGCAAATTGTTCCAATTTCAAAAAACAGTCTTTCTGGGGAAGTGATTGGTCAACTGTTACATTCCA
TTATGAGCGAAAAACAACGCAAAGAATTTGCAGAAACTCGTGAATGTAACTTTGCCATTATGAACCGTGAAAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAGCGCGACATGCCAGGCATGGTACTACGTCGTATTGAAACCAAGATTCCTTCAAT
TGATGACTTGCAGTTACCGCCTGTACTTAAAGATTTATCGATGACCAAACGCGGCATTATTATTTTCGTAGGCGCGACAG
GCACAGGTAAATCTACTTCATTGGCTTCAATGATCAGCCATCGTAACCATAATTCTAAGGGCCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCACGAACATGCAGGCTGCATCATTACTCAGCGTGAAGTCGGGATCGATACCGACTCATTTGA
AATTGCCTTGAAAAATACACTACGACAAGCGCCGGATGTGATCTTAATTGGTGAGATCCGTTCTCGTGAAGTCATGGACT
ACGCGATTGGCTTTGCTGAAACAGGCCATCTTGTATTAGCTACCATGCACGCTAACAATGCCAACCAAGCGCTCGACCGT
ATTATTCACTTTTTTGAAAGTGATCGTCACAGTCAGCTTTACATGGACTTGTCACTTAACTTAAAGGCAATGATTGCGCA
GCAGCTCATTCCAACACCAGATGGTAATTCACGCCGTGCAGCAATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAAGATTTGATGAAACGTTCACGTGAACTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATATAAAGCTGGTCAAATTACCTACAAAGATGCACTTAAACATGCTGACTCACCGAACGATTTACG
TTTAACCATTAAACTTGCTGATGAAGGTCCTGATCAATTATCAGACGGGAAACAGCATTTAACTTTTGACCGCCAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (31-270)

3D structure

• AlphaFold DB: A0A009PAD6

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.926	100	0.772
pilU	Pseudomonas stutzeri DSM 10701	69.293	98.925	0.685
pilU	Vibrio cholerae strain A1552	53.243	99.462	0.53
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.197	93.011	0.392